Antemortem diagnosis of rabies from skin by TaqMan real time PCR

Bansal, K.; Singh, Charan; Sandhu, B.S.; Sood, Naresh Kumar; Dandale, M.

doi:10.5958/0976-0555.2014.00038.7

Cited by 5 publications

(3 citation statements)

References 9 publications

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“…Saliva and nuchal skin biopsy are proved to be diagnostic in animals. (9)(10)(11)(12) Nuchal skin biopsy is reported to be more widely accepted sample for ante-mortem rabies diagnosis in humans and animals. (3,(13)(14)(15)(16)) However sampling is difficult and risky in live rabies suspected animals and hence there are only limited studies evaluating its utility as an ante-mortem tool in animals (3,6,17) and only very few studies regarding its effectiveness as a post mortem sample.…”

Section: Discussionmentioning

confidence: 99%

Skin Specimen as an Alternative to Brain Tissue for Post Mortem Rabies Diagnosis in Animals to Scale up Animal Rabies Surveillance

Aparna¹,

Swapna²,

Ajithkumar³

et al. 2022

EAS J Vet Med Sci

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Background: Detection of the virus or some of its specific components using WHO and OIE recommended standard laboratory tests is the only way to get a reliable diagnosis of rabies. Brain tissue is the preferred specimen for post-mortem diagnosis of rabies in both humans and animals. Higher biosecurity requirements, skill and transportation facilities required for collection and transport of brain or whole carcass to the laboratory is one of the reasons for the poor rabies surveillance in animals. Point of care testing with simple, reliable and easy to operate devices would be an ideal approach for providing rapid results. Methods: The study evaluated diagnostic performance of two reference tests, DFAT and RTPCR on skin specimen, to assess its suitability as an alternative of brain tissue for post mortem rabies diagnosis in animals. Brain tissue and skin sample belonging to different species of animals (n=90) collected at necropsy were compared using Fluorescent Antibody Test and RT PCR, internationally approved methods for rabies diagnosis. Results: Validation of RT-PCR on skin and DFAT on skin in comparison with DFAT on brain as gold standard gave a sensitivity of 98% (95% CI:94.1-100) and 80% (95%CI:71.8-88.2) respectively. Specificity was 100% in both tests. Conclusion: The findings highlight the potential of skin specimen for improving rabies surveillance in animals especially in resource poor countries.

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Section: Discussionmentioning

confidence: 99%

Skin Specimen as an Alternative to Brain Tissue for Post Mortem Rabies Diagnosis in Animals to Scale up Animal Rabies Surveillance

Aparna¹,

Swapna²,

Ajithkumar³

et al. 2022

EAS J Vet Med Sci

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“…These cells have broad clinical application prospects in tissue engineering, such as bone, cartilage, muscle, tendon, ligament, nerve, liver, endothelium and myocardium (Wang et al 2017). MSCs have been isolated from the human umbilical cord and their cell content and proliferative ability are better than those of bone marrow mesenchymal stem cells (BMSCs) (Bansal et al 2014;Rahman et al 2015). They have lower immunogenicity than bone marrow MSCs and have the advantages of convenient materials and no ethical controversy.…”

Section: Dir Marking Of Uc-mscsmentioning

confidence: 99%

Tree Shrew Umbilical Cord Mesenchymal Stem Cells Labeled with the Dark Red Fluorescent Dye DIR and Small Animal Live Imager Observation

Ruan¹,

Yao²,

Wang³

et al. 2021

IJAR

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Background: Umbilical cord mesenchymal stem cell transplantation can treat metabolic syndrome, but the tracing of cells in the body after transplantation has always been a problem. Tree shrew umbilical cord mesenchymal stem cells were labeled with the dark red fluorescent dye DIR and a metabolic syndrome model in tree shrew was generated. The migration, distribution, colonization and survival of the cells were observed. Methods: Tree shrew umbilical cord mesenchymal stem cells were labeled with the dark red fluorescent dye DIR. Three days after the tree shrew model was generated, the pancreas, kidney and liver were placed in a small animal live imager to observe the distribution of the labeled cells. Result: The labeled cells showed deep red fluorescence in the live imager. After treatment with the transplanted cells, dark red fluorescent signals were observed in the liver, kidney and pancreas of the tree shrews but not in the untreated group and no dark red fluorescent signal was observed in the cell distribution.

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“…Rabbits have significant economic importance, both in livestock and pet industry (DG Health and Food Safety, 2017). Moreover, this species is also used frequently as an animal model for studying infectious (Bansal et al, 2014;Peng et al, 2015) and non-infectious diseases (Anjum et al, 2019;Fan et al, 2014), due to several advantages such as easier housing and handling over larger species of animals. Blood parameters represent a great diagnostic tool for animal health status and disease evaluation (Barkakati et al, 2015;Etim et al, 2013;Kumar et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Reference Values for Selected Blood Parameters in Rabbits: Effects of Age and Physiological Status

Argente

Abad-Salazar

Bermejo-González³

et al. 2019

IJAR

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Rabbit is widely used as an experimental animal model in infectious and non-infectious diseases. The haematologic data can be helpful in evaluating the health status of animals over time. The aim of this study was to determine the levels of red blood cells (RBC), white blood cells (WBC) and differential leukocyte counts in 5 nulliparous and 5 multiparous females, i.e. in young and older animals, at mating and at delivery. The values of RBC did not change with age, but WBC and lymphocytes decreased with age, a -33% and a -60% less in multiparous females than nulliparous ones. Monocytes count was double at delivery than at mating. In conclusion, aging on the immune system is manifested as reduction in production of mature lymphocytes and as a result, older females would not respond to immune challenge as robustly as the young ones. Physiological status is only related to production of monocytes.

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Antemortem diagnosis of rabies from skin by TaqMan real time PCR

Cited by 5 publications

References 9 publications

Skin Specimen as an Alternative to Brain Tissue for Post Mortem Rabies Diagnosis in Animals to Scale up Animal Rabies Surveillance

Skin Specimen as an Alternative to Brain Tissue for Post Mortem Rabies Diagnosis in Animals to Scale up Animal Rabies Surveillance

Tree Shrew Umbilical Cord Mesenchymal Stem Cells Labeled with the Dark Red Fluorescent Dye DIR and Small Animal Live Imager Observation

Reference Values for Selected Blood Parameters in Rabbits: Effects of Age and Physiological Status

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