Ante mortem diagnosis of rabies from body secretion/excretion by TaqMan real time PCR

Dandale, M.; Singh, Chanchal; Ramneek,; Deka, D; Sandhu, B.S.; Bansal, K.; Sood, Naresh Kumar

doi:10.5455/ijavms.155

Cited by 1 publication

(1 citation statement)

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“…Ante-mortem and post-mortem rabies diagnosis by RT-PCR has been demonstrated (David 2012). TaqMan real time-PCR was found to be quite sensitive for the diagnosis of rabies from body secretion/excretion (Dandale et al 2012(Dandale et al , 2013Mani et al 2014). Absence of vaccineassociated rabies cases following oral vaccination was demonstrated with the help of pyrosequencing of the RVG gene (De Benedictis et al 2013).…”

Section: Detection Of Viral Nucleic Acidmentioning

confidence: 99%

Rabies – epidemiology, pathogenesis, public health concerns and advances in diagnosis and control: a comprehensive review

Singh

Cherian

et al. 2017

Veterinary Quarterly

201

182

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Rabies is a zoonotic, fatal and progressive neurological infection caused by rabies virus of the genus Lyssavirus and family Rhabdoviridae. It affects all warm-blooded animals and the disease is prevalent throughout the world and endemic in many countries except in Islands like Australia and Antarctica. Over 60,000 peoples die every year due to rabies, while approximately 15 million people receive rabies post-exposure prophylaxis (PEP) annually. Bite of rabid animals and saliva of infected host are mainly responsible for transmission and wildlife like raccoons, skunks, bats and foxes are main reservoirs for rabies. The incubation period is highly variable from 2 weeks to 6 years (avg. 2-3 months). Though severe neurologic signs and fatal outcome, neuropathological lesions are relatively mild. Rabies virus exploits various mechanisms to evade the host immune responses. Being a major zoonosis, precise and rapid diagnosis is important for early treatment and effective prevention and control measures. Traditional rapid Seller's staining and histopathological methods are still in use for diagnosis of rabies. Direct immunofluoroscent test (dFAT) is gold standard test and most commonly recommended for diagnosis of rabies in fresh brain tissues of dogs by both OIE and WHO. Mouse inoculation test (MIT) and polymerase chain reaction (PCR) are superior and used for routine diagnosis. Vaccination with live attenuated or inactivated viruses, DNA and recombinant vaccines can be done in endemic areas. This review describes in detail about epidemiology, transmission, pathogenesis, advances in diagnosis, vaccination and therapeutic approaches along with appropriate prevention and control strategies.

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