The article presents the materials of the study on bull cross of the Bredy meat type simmentals, which include the genotyping of animals for the CAST and CAPN1 genes with the identification of frequencies of genotype and alleles, as well as testing the production of these animals expressed in the evaluation of aged within 8 days of meat for the indicators tenderness, juiciness, organoleptic assessment of taste. The results of the studies did not confirm, with certainty, the influence of the CAST gene polymorphism on the beef tenderness indicators, as well as no associations of this gene with the studied juiciness and meat taste indicators, while the carriers of the homozygous CC genotype CAPN1 exceeded other genotypes of the sample in tenderness, juiciness and taste of beef. And indicators of bull meat with a combination of TT* genes on CAST and CC* on CAPN1 had superiority over the average indicators of the total sample (P <0.001), as well as over the group of animals with the most preferred CC genotype for the CAPN1 gene by 1.22 points or 15.4% (P <0.01) by tenderness, by 1.44 points or 18.2% (P <0.05) by juiciness, by 1.56 points or 19.2% (P <0.001) by taste. Studies have confirmed the effect of the CAPN1 gene CC homozygotes polymorphic state itself, as well as the CAST and CAPN1 genes on beef tenderness in the part where the combination of TT genotypes in the CAST gene and CC in the CAPN1 gene gives a positive effect on beef tenderness, probably associated with activation the activity of μ-calpain in connection with the weakening of the effect of calpastatin as an inhibitor on μ-calpain, as a result of which the enzymatic effect on myofibrillary proteins acquires a different intensity, and possibly a different manifestation. What was the reason for the increase in sensory sensitivity to taste of prepared samples, tenderness and juiciness of meat.
A comparative study of the biological characteristics of the heifers Bredy meat type of two populations located in Russia and Kazakhstan was carried out to determine the state of genes associated with the meat quality in the genotypeCAPN1, GDF5, TG5, BGL. In genotyping, we used the real-time PCR method using oligonucleotide primers (Gen Bank), for each primer individually in the corresponding sequences. Studies for improve breeding by innovative methods revealed the frequencies of genotypes and alleles for marker genes CAPN1, TG5, BGL and GDF5, which are related to meat quality indicators, a certain displacement in the genotypes of the compared populations of the Bredy meat type of Kazakhstan and Russia according to the CAPN1 gene (χ2 = 8.52) and the TG5 gene (χ2 = 4.73). The genotyping result of animals from LLC “Breeding plant Bredy” were analyzed from the point of view of attributing carriers of different genotypes to the offspring of bulls-producers Fakir, Kust and Chizhik, in order to determine the producer giving offspring with predictably high nutritional and biotechnological properties of beef. A comparison of the carriers of the identified genotypes of the studied genes by the characteristics used in the evaluation of young animals by their own productivity did not give significant results, however, showed trends by which scientific research can be directed. An assessment of the bulls-producers of the successors of the different line of meat simmentals on the quality of heifers – daughters showed that the largest comprehensive index was set for the bull-producer Bush 39046 – 102.7, which was recognized as an improver. The assignment of all, without exception, heifers of different groups, evaluated bulls-producers, to the highest class elite-record, indicates the value of all the studied genotypes.
Федеральный научный центр биологических систем и агротехнологий Российской академии наук» (г. Оренбург) 2 Оренбургский государственный аграрный университет» (г. Оренбург) 3 Институт животноводства Таджикской академии сельскохозяйственных наук (Республика Таджикистан) Аннотация. Проведено сравнительное изучение биологических характеристик молодняка брединского мясного типа двух популяций, находящихся в России и Казахстане, на предмет определения в генотипе состояния аллельных форм некоторых маркерных генов и элементного статуса по шерсти в зависимости от места разведения. При генотипировании был применён метод ПЦР в реальном времени с использованием олигонуклеотидных праймеров (GenBank), по каждому праймеру в отдельности в соответствующих последовательностях. Элементный состав определялся в лабораторных условиях на образцах шерсти животных с использованием квадрупольного массспектрометра методом масс-спектрометрии с индуктивно связанной плазмой (МС-ИСП). Исследованиями выявлены частоты генотипов и аллелей по маркерным генам CAPN1, TG5, BGL, имеющим отношение к качественным показателям мяса, при этом обнаружено некоторое смещение генотипов сравниваемых популяции по гену CAPN1 (ᵪ 2 =8,52) и по генуTG5 (ᵪ 2 =4,73). Обнаружена разница по элементному составу шерсти животных разных популяций, обусловленная депрессивным влиянием на окружающую среду, а также последствиями деятельности токсичных производств в одном из рассматриваемых зон. Отмечается, что показатели по содержанию Со, Cr, Ni, Рb, V превышают эталонные интервалы для зоны Южного Урала, разработанные нашими учёными, от 1,6 раза по Со до 5,7 раза-по V. Согласно данным нормам, содержание химических элементов в шерсти животных ООО «Совхоз Брединский» соответствовало выявленным центилиям (25-75 %). Ключевые слова: крупный рогатый скот, симментальская порода, брединский мясной тип, молодняк, ген, генотип, аллель, частота генотипов и аллелей, шерсть, химический элемент.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.