Monoclonal antibodies detect evident antigenic variations in NDV HN and F protein. However, the A/PMV-1 viruses can be identified by HI test using a preparation made of the combination of two different monoclonals. A primary evaluation of the pathogenicity of the isolated viruses can be made by HI test using monoclonal antibodies but needs always confirmation using conventional pathogenicity tests.
Individual variations in serological response to avian influenza virus infection were demonstrated after experimental infection of specific-pathogen-free chickens with H6N2 influenza virus. Homologous antibodies were detected from the 6th to the 157th day after infection using hemagglutination-inhibition or enzyme-linked immunosorbent assay and from the 11th to the 157th day by agar gel precipitation test.
Chickens were immunised using a vaccinia recombinant virus (vaccinia-Italien-F), expressing the F protein of Newcastle disease virus (NDV). Immunisation was successful using either TK" cells infected with the vaccinia-Italien-F virus, the recombinant virus grown in TK7 cells and inoculated intra-cerebrally in one-day-old chickens or the recombinant virus given by wing-web to adult chickens after adaptation by alternate passage in chick embryo fibroblasts and chickens. The use of recombinant viruses expressing the F protein of NDV as vaccines would allow joint application of vaccination and eradication programmes for NDV. Therefore, recombinant viruses obtained in chickens virus vectors are needed.
21 A/PMV-1 viruses were isolated from pigeons and characterized using polyclonal and monoclonal antisera in hemagglutination inhibition, seroneutralization and immunoprecipitation studies. Polyclonal and monoclonal antibodies directed against the HN and F proteins of Italien virus reacted with all pigeon isolates showing a close relationship between chicken velogenic and pigeon viruses. Differences in the M.W. of F0, P and M proteins were however observed between pigeon and chicken Italien virus. Marked differences in virulence were recorded among pigeon isolates; these were reflected by great variation in the IVPI of the different strains.
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