Antigenic and biological characterization of avian paramyxovirus type 1 isolates from pigeons

Abstract

21 A/PMV-1 viruses were isolated from pigeons and characterized using polyclonal and monoclonal antisera in hemagglutination inhibition, seroneutralization and immunoprecipitation studies. Polyclonal and monoclonal antibodies directed against the HN and F proteins of Italien virus reacted with all pigeon isolates showing a close relationship between chicken velogenic and pigeon viruses. Differences in the M.W. of F0, P and M proteins were however observed between pigeon and chicken Italien virus. Marked differences in virulence were recorded among pigeon isolates; these were reflected by great variation in the IVPI of the different strains.

Evolution of pigeon Newcastle disease virus strains

“…(Collins et al, 1996) were included. Strain 1489 was isolated in Belgium in 1984 (Meulemans et al, 1986).…”

“…The NDV isolates obtained from diseased pigeons, termed "pigeon PMV-1" (PPMV-1), have been characterized by pathogenicity tests, sequencing of the F protein and monoclonal antibody binding studies (Meulemans et al, 1986;Alexander et al, 1993;Collins et al, 1993Collins et al, , 1994Collins et al, , 1996. Common features of these isolates were that they were antigenically indistinguishable from each other, but different from NDVs (avian PMV-1) isolated in poultry, that they had a mean intracerebral pathogenicity index (ICPI) of 1.44, and that most of them possessed the motif 112 GRQKRF 117 at the F2/F1 cleavage site of the F protein.…”

Protective effects of HN and F glycoprotein‐specific monoclonal antibodies on experimental newcastle disease

“…Four of the five anti F monoclonal antibodies neutralised the virus to high titres whereas the 5th (monoclonal 2C1) had low neutralising activity. Meulemans et al (1986a). b Le Long etal.…”

“…The cells were grown and maintained in Dulbecco modified Eagle medium (DMEM). The techniques used for performing the neutralisation assays have already been described (Le Longetal., 1986;Meulemans et al, 1986a).…”

“…Rabbits were injected several times with octylglucoside solubilised glycoproteins from NDV Italien (Meulemans et al, 1986a). Six-week-old chickens in isolators were injected with a commercial inactivated vaccine containing the velogenic strain 'Poletti' (Newcavac Intervet) and bled 4 weeks later.…”