One of the current trends in dietary preferences involves the transition to a low-or reduced-meat diet, which is often desirable for health and environmental reasons. This change in dietary preferences requires an in-depth insight into consumers´ preferences towards a variety of alternative/non-meat proteins. This study aimed to investigate the consumers' preferences and willingness to purchase three alternative dietary protein sources, namely plant-, cultured meat-and insect-based proteins in four countries with dissimilar economic development status (the United Kingdom, Spain, Brazil and the Dominican Republic). It also aimed to determine which factors would most influence the willingness to purchase. From a total sample of 729 valid respondents, psychographic variables were analysed. The alternative protein deemed the respondents' most preferred willing to purchase was the plant-based type since that option tended to be more widely available in the market. Among the analysed economic groups, the countries classified in the higher economic groups tended to show more readiness to replace traditional meats for the three alternatives. Models suggest that the respondents regarded the alternative characteristics and/or the attributes compared to meat as being the most important factors that influence their willingness to purchase rather than environmental, convenience or healthy buying decisions, or a low level of neophobia. If the perception of healthiness, safety and nutritiousness increases one-unit for the cultured meat in Brazil, the probability of willingness to purchase would increase 86.82%. One-unit stronger belief in Spanish that plant-based are healthy, safe and nutritious higher the probability of willingness to purchase 68.74%. One-unit higher perceive the characteristics of healthiness, safety and nutritional content of the insectsbased products would increase 68% the probability of willingness to purchase in the United Kingdom, 72% in Brazil and 58% in the Dominican Republic.

Insects as an alternative protein source has gained traction for its advantageous environmental impact. Despite being part of many traditional food cultures, insects remain a novelty in Western cultures and a challenging concept for many. Even though plant-based protein alternatives are not facing the same barriers, product unfamiliarity and limited exposure hinder adoption, which could be detrimental to growth within the food sector. This study is aimed at evaluating plant- and insect-based proteins as alternative dietary proteins. A model indicating the drivers of consumer attitudes towards meat-alternative proteins and consumer willingness to try, buy, and pay a premium was tested. Further, 3091 responses were collected using surveys in nine countries: China, USA, France, UK, New Zealand, Netherlands, Brazil, Spain, and the Dominican Republic. Structural Equation Modelling was used to analyze the data. We found that consumer’s behavioral intentions towards both plant-based and insect-based alternatives are inhibited by food neophobia but to an extent, are amplified by the perceived suitability and benefits of the protein, which in turn are driven by nutritional importance, environmental impact, healthiness, and sensory attributes for both alternatives. The expectation of the nutritional value of meat is the strongest (negative) influence on perceived suitability/benefits of plant-based protein and willingness to try, buy, and pay more for plant-based proteins, but it only has a relatively small impact on the suitability/benefits of insect-based protein and no impact on willingness to try, buy, and pay more for insect-based proteins. Overall, we conclude that consumer adoption towards meat alternatives is complex and is strengthened by the perceived suitability/benefits of the protein and general importance of perceived food healthiness and sustainability. Conversely, adoption is hindered by dietary factors and the experiential importance of meat and food neophobia.

Much of medical research relies on animal models to deepen knowledge of the causes of animal and human diseases, as well as to enable the development of innovative therapies. Despite rodents being the most widely used research model worldwide, in recent decades, the use of the zebrafish (Danio rerio) model has exponentially been adopted among the scientific community. This is because such a small tropical freshwater teleost fish has crucial genetic, anatomical and physiological homology with mammals. Therefore, zebrafish constitutes an excellent experimental model for behavioral, genetic and toxicological studies which unravels the mechanism of various human diseases. Furthermore, it serves well to test new therapeutic agents, such as the safety of new vaccines. The aim of this review was to provide a systematic literature review on the most recent studies carried out on the topic. It presents numerous advantages of this type of animal model in tests of efficacy and safety of both animal and human vaccines, thus highlighting gains in time and cost reduction of research and analyzes.

Vegetable oil crop production has increased faster than other food or industrial crops in recent years. Palm oil which is widely grown in tropical areas has become the most internationally produced and traded oil with detrimental effect to the natural environment and the sustainability of producing communities. The general perception of the industry conveys a negative image to consumers. To better understand the extent consumers' perception of palm oil products would inform stakeholders in the sector 25 one-to-one interviews using the means-end laddering technique (Olson and Reynolds, 2001) were carried out to elicit attributes which led to the establishing of value perceptions of products containing palm oil. Pal production practices have elicited negative Environmental Concern Values regarding the availability of resources for future generations. Environmental consciousness seems to be an important attribute marketers should take into account when both developing and promoting products. However, consumers' perception of palm oil as a product ingredient was regarded as minimal. Palm oil was perceived as being a more natural product whose naturalness could be exploited regarding 'physical wellbeing' values leading helping consumers achieve a health life and higher selfstem.

In this study we investigate the consumer's willingness to try innovative food products in the context of the metropolitan area of Porto Alegre in Brazil and Cirencester in England, UK. Innovation in the food industry is an important source of differentiation and a value-adding opportunity for managers to develop new products. Therefore, the adoption or rejection of innovative food products becomes strategic from a market point-of-view. Using the Domain Specific Innovativeness [DSI] scale and the Food Neophobia Scale [FNS], two surveys were carried out in Brazilian and British universities with 279 and 101 respondents, respectively. Consumers were not the most inclined to adopt innovations, but they were not afraid of new foods either, especially in the UK. Managers in the food industry could be missing out on opportunities to innovate more. The results provide strategic and unique information about consumers for the food industry, aiming at supporting the development of innovative food products.

The present research aimed to examine the chemical and microbiological characteristics of refrigerated raw milk collected from buffalo-only dairy herds from different farms and seasons. Samples were collected from twelve farms producing buffalo milk, located in the Mideast region of São Paulo state, in Brazil, once a month throughout the dairy calendar year from 2011 to 2014. The analysis of fat, protein, lactose, dry extract, and total solids contents were carried out using Infrared Absorption. Residues for antimicrobial analysis were carried out using Delvo Test Kit technique, and both somatic cell count and standard plate count analysis were carried out using Flow Cytometry. The analysis of the chemical components as well as for the microbiological analysis of SPC proved to be statistically significant throughout the period. No antibiotic residues were found in the totality of the sample, hence indicating that the farmers complied with the current sanitary legislation in that country, as well as SCC; however, higher values were found for the SPC. Significant results for all parameters analyzed were found among the farms, indicating that price incentives paid for the quality of raw milk could be an alternative to dairy processing companies, favoring yield and quality of the final product. Keywords: Brazil; chemical parameters; dairy; microbiological parameters; monitoring. ResumoO presente estudo objetivou examinar as características químicas e microbiológicas do leite cru refrigerado coletado em diferentes fazendas e estações. Amostras foram coletadas em doze fazendas 2 Cienc. anim. bras., Goiânia, v.18, 1-12, e-41815, 2017Quality of refrigerated raw milk from buffalo cows… produtoras de leite de búfala localizadas na região centro-leste do estado de São Paulo, Brasil, uma vez ao mês entre os anos de 2011 a 2014. Análises de gordura, proteína, lactose, extrato seco e sólidos totais foram realizadas utilizando-se técnica de Infra-vermelho. Análises de resíduos de antimicrobianos foram realizadas através do teste rápido Kit Delvo Test. Análises de contagem de células somáticas e contagem padrão em placas foram conduzidas através de Citometria de Fluxo. Resultados das análises dos componentes químicos, assim como da análise microbiológica de CPP, demonstraram diferenças estatísticas significativas através das estações para estes parâmetros. Em todas as amostras analisadas nenhum resíduo de antimicrobiano foi detectado, indicando que os produtores estão respeitando a legislação sanitária brasileira vigente, bem como para SCC; no entanto, valores acima do permitido foram encontrados para CPP. Diferenças significativas para todos os parâmetros analisados foram encontradas entre as fazendas investigadas, indicando que o incentivo de preço pago pela qualidade do leite cru poderia ser uma alternativa às empresas de processamento de lácteos, favorecendo o rendimento e a qualidade do produto final. Palavras-chave: Brasil; laticínio; monitoramento; parâmetros químicos; parâmetros microbiológicos.

The objective of this study was to evaluate the potential of in vivo bioimpedance analysis (BIA) as a method to estimate body composition in lambs. Thirty-one Texel x Ile de France crossbreed ram lambs were slaughtered at pre-determined intervals of average weights of 20, 26, 32, and 38 kg. Before the slaughter of the animals, their body weight (BW) and body length (BL) were measured. The values for resistance (Rs) and reactance (Xc) were collected using a single-frequency BIA equipment (Model RJL Quantum II Bioelectrical Body Composition Analyzer). The BIA main variables such as body bioelectrical volume (V), phase angle (PA), resistive density (RsD), and reactive density (XcD) were then 2 calculated. The soft tissue mass of the right-half cold carcass was analyzed in order to determine its chemical composition. Multiple regression analyses were performed using the lamb body composition as dependent variables and the measurements related to bioimpedance as independent variables. The best regression models were evaluated by cross-validation. The predictive model of moisture mass, which was developed by using XcD and V, accounted for 84% of its variation. Resulting models of percentage moisture (R 2 = 0.79), percentage lean mass (R 2 = 0.79), percentage fat (R 2 = 0.79), and fat mass (R 2 = 0.87) were obtained using RsD and V. Furthermore, the values of RsD regarding V, and PA in the prediction models accounted for 91% and 89% of variation in protein mass and lean mass, respectively. Bioimpedance analysis proved to be an efficient method to estimate the body composition of lambs slaughtered at different body mass stages.

Toxicity studies in mammals continue to be the most appropriate model for predicting risk in humans, but they tend to be expensive and time-consuming. In the aftermath of the genetic sequencing of zebrafish (Danio rerio), that species showed to be highly genetically homologous to humans. The use of the zebrafish model to assess food toxicity is already a reality as it is capable of biological processes difficult to reproduce in vitro. Studies of complex mechanisms of absorption, distribution, metabolism and excretion as well as cellular and tissue interactions are of great information value resulting in time, space and cost savings, when compared to studies with rodents. This review addresses the relevance of zebrafish model in food safety research, both in the use of ingredients and innocuous food additives as well as for establishing levels of safe food contaminant residues present in the environment. Toxicological screening using the zebrafish model integrate the evaluation of teratogenicity, cardiotoxicity, hepatotoxicity, genotoxicity, neurotoxicity, endocrinetoxicity, reproductive and behavioral aspects. These are important endpoints for food safety assessment, which take substantially less time than in mammalian tests. Furthermore, it serves well as a screening test follow-up for validating favorable results in murine models, hence accelerating the risk assessment process of products submitted for approval and registration, prioritizing safe compounds and reducing unnecessary costs in subsequent mammalian studies. In conclusion, zebrafish model can be a useful tool for food safety tests, however, additional studies are needed to further validate this model for registration of new food ingredients and additives.