The voltammetric oxidation of all deoxyribonucleic acid (DNA) monophosphate nucleotides is investigated for the first time over a wide pH range by differential pulse voltammetry with a glassy carbon electrode. Experimental conditions such as the electrode size, supporting electrolyte composition, and pH were optimized to obtain the best peak potential separation and higher currents. This enabled the simultaneous voltammetric determination of all four DNA bases in equimolar mixtures and detection limits in the nanomolar range at physiological pH. It was also possible to detect for the first time the oxidation of each of the purine and pyrimidine nucleotides free in solution or as monomers in single-stranded DNA.
-The present study was carried out in Northeastern Region of Brazil, in order to investigate the use of stingless bee Melipona subnitida Ducke in the pollination of greenhouse sweet pepper (Capsicum annuum L.). Treatments of hand cross-pollination, hand self-pollination, pollination by bees and restricted pollination were performed. Results showed that despite sweet pepper flowers are considered autogamous, this crop benefits from pollination by M. subnitida, producing fruits significantly heavier and wider, containing a greater number of seeds and of better quality (lower percentage of malformed fruits) than self-pollinated sweet pepper. Thus, M. subnitida can be considered an efficient pollinator of greenhouse sweet pepper.Index terms: meliponin, caged environment, sweet pepper flower.
Eficiência de polinização da abelha sem ferrão Melipona subnitida na cultura do pimentão em casa de vegetaçãoResumo -O objetivo deste trabalho foi investigar o uso da abelha sem ferrão Melipona subnitida Ducke, na polinização de flores de pimentão (Capsicum annuum L.), sob cultivo protegido, na Região Nordeste do Brasil. Foram comparados tratamentos de polinização cruzada manual, polinização por abelhas, autopolinização manual e polinização restrita. Os resultados mostraram que apesar de as flores de pimentão serem consideradas autógamas, essa cultura beneficia-se da polinização realizada por M. subnitida, produzindo frutos significativamente mais pesados e mais largos, com um número maior de sementes e de melhor qualidade (baixo porcentual de frutos deformados), quando comparada com a cultura autopolinizada. Portanto, M. subnitida pode ser considerada uma polinizadora eficiente de pimentão em casa de vegetação.Termos para indexação: meliponineos, cultivo protegido, flor de pimentão.
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