Snakes have an important biological role in the ecological chain, as well as in scientific researches performed with the venoms produced by them, since the enzyme-protein fractions these substances possess have been studied as pharmacological tools for the discovery of new therapies. Snakes of the genus Crotalus have gained significant relevance in the scientific field, since the venom produced by these reptiles has been the target of researchers in a few decades, due to the composition and the effects that these substances can produce. In Brazil, a single species represents the genus, which is Crotalus durissus. This review demonstrates that even with the advancement in scientific research on the composition, role and application of the venom produced by the subspecies of Crotalus durissus snake, it is necessary to further study their fractions and their action potential, which also demonstrates the how rich are these active components in different fields of biomedicine.

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Most cases of Hepatitis C are asymptomatic and symptoms appear when the disease is already hepatic. The present study aimed at the production and purification of the Hepatitis C virus core protein (HCV) in the prokaryotes system and its subcloning in eukaryonite expression vectors. The E. coli bacterium was selected to perform the synthesis of the protein of interest, which was purified by affinity chromatography and detected by western blotting. In addition, the sequence of interest was recombined in plasmids for targeting to plant cell sub-compartments, which were confirmed by PCR. The processes performed were successful and the subsequent expression in the different cell compartments will allow the best performance for the production of immunogens and enable the production of diagnostic kit.

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