RESUMOEstudou-se o efeito da suplementação com misturas múltiplas sobre o consumo, digestibilidade e desempenho de novilhos Nelore, em pastagens de Brachiaria brizantha Cv. Marandu, submetidos a quatro tratamentos. No tratamento um (T1), cada animal recebeu 800g/dia de suplemento contendo uréia como fonte de nitrogênio não protéico (NNP); no tratamento dois (T2), recebeu 800g/dia de mistura na qual a uréia foi substituída por amiréia como fonte de NNP; no tratamento três (T3), recebeu 1500g/dia de uma mistura com amiréia; e no tratamento quatro (T4= controle), recebeu sal mineral. Seis animais por tratamento foram utilizados para se medir o consumo pela técnica de indicador externo (óxido crômico), e 10 animais foram usados na avaliação de ganho de peso. Foram utilizados dois animais canulados no esôfago para coleta de amostra de extrusa. Os animais suplementados obtiveram ganhos de peso superiores (P<0,05) aos do tratamento-controle, 435, 419, 467 e 271 g/dia para os tratamentos T1, T2, T3 e T4, respectivamente. Os coeficientes de digestibilidade da matéria seca (MS) foram de 56,7, 49,8, 48,9 e 45,5% para os tratamentos T1, T2, T3 e T4, respectivamente, sendo correlacionados positivamente com a digestibilidade in vitro da MS (P<0,05). A suplementação com misturas múltiplas promoveu aumento no consumo (P<0,05).Palavras-chave: bovino, amiréia, mistura múltipla, suplementação 56.7, 49.8, 48.9 and 45.5%, respectively, for T1, T2, T3 and T4. A ABSTRACT
This study evaluated the use of cooled semen in a fixed-time artificial insemination (FTAI) program compared with frozen-thawed semen to improve pregnancy rates in beef cattle. Ejaculates of three bulls were collected and divided into two treatments: (1) frozen-thawed semen and (2) cooled semen. Egg-yolk extender without glycerol was used for the cooled semen treatment. Straws (25×10 spermatozoa) were submitted to cooling for preservation at 5°C for 24h, after which FTAI was performed. Nelore cows (n=838) submitted to FTAI were randomly inseminated using frozen-thawed semen or cooled semen. There was a 20% increase in the pregnancy per AI (P AI) using cooled semen compared with frozen-thawed semen (59.9±4.7 vs 49.4±5.0%; P<0.005). There was no difference in P AI among the bulls (P=0.40). The frozen-thawed semen had fewer functional spermatozoa than did the cooled semen when evaluated by sperm motility (61.7 vs 81.0%), slow thermoresistance test (41.7 vs 66.7%) and hypoosmotic swelling test (38.3 vs 53.7%; P<0.05). The percentage of sperm abnormalities did not differ between the freeze-thawing and cooling processes (18.6 vs 22.1%; P>0.05). Because there was less damage to spermatozoa and improvement in P AI, the use of cooled semen instead of frozen-thawed semen is an interesting approach to increase reproductive efficiency in cattle submitted to a FTAI protocol.
RESUMO -Avaliaram-se os efeitos dos níveis de nitrogênio de suplementos proteicos sobre as concentrações de nitrogênio amoniacal (N-NH 3 ) e ácidos graxos voláteis (AGV) e o pH em bovinos de corte em pastagem de capim-marandu (Brachiaria brizantha, cv. Marandu). Foram realizadas estimativas da síntese microbiana, do aporte de proteína não-degradável no rúmen (PNDR) e proteína endógena e das suas contribuições no pool de proteína metabolizável (PM). Quatro bovinos Nelore com 395 ± 9 kg, fistulados no rúmen, foram utilizados nas medidas dos parâmetros ruminais e nas avaliações da degradabilidade, da cinética ruminal e das estimativas de síntese microbiana em um delineamento quadrado latino 4 × 4.Suplementos com 30, 40 ou 50% de proteína bruta (PB) foram fornecidos na quantidade de 400 g/animal.dia para comparação a um grupo controle, sem suplementação proteica. Os animais foram mantidos em pastagens de Brachiaria brizantha, cv. Marandu, distribuídos em quatro piquetes com área de 1,0 ha cada, com oferta do suplemento e retirada das sobras, realizada diariamente. As concentrações de N-NH3 nos animais que receberam o suplemento com 50% PB foram superiores às observadas naqueles sob suplementação com 40% PB e no grupo controle, mas foram semelhantes às observadas no grupo sob suplementação com 30% PB. As concentrações de AGV no grupo sob suplementação com 30% PB foram superiores às observadas no grupo controle e semelhantes às obtidas com suplementação com 40 e 50% PB.O pH não diferiu entre os grupos. A estimativa de oferta de proteína microbiana e de PNDR foi maior para os animais sob suplementação com proteína em relação à observada no grupo controle. Brachiaria brizantha cv Marandu, distributed in four one-hectare paddocks where the supplements were offered and the orts removed daily. The N-NH 3 levels in the animals that received 50% CP were higher than those observed in animals receiving 40% CP and C, but were similar to the levels observed in the animals supplemented with 30% CP. The VFA concentrations in the group supplemented with 30% PB were higher than the control treatment (C) and similar to those obtained with 40 and 50% CP supplementation. The pH did not differ among the groups. The microbial synthesis and RUP were greater for the animals that received protein supplementation compared to the control treatment.
Bovine livestock is a major anthropogenic greenhouse gas source via enteric methane. Brazilian bovine livestock is also responsible for emissions from land-use changes. In contrast, enteric emissions from extensive cattle systems in wetlands might have been overestimated. We provide scientific evidences that the human footprint of bovine products delivered by the Pantanal can be much lower. To assess this, a historical cloud-free imagery of the Landsat-5, spanning 26 years, were processed for mapping spatiotemporal landscapes in a Pantanal farm under cattle intensification studies. Eight landscape categories were identified according to spatiotemporal dynamics of interannual floods. The spatiotemporal map allowed in the field the adoption of stratified random samplings of chamber gas fluxes. The combination of stratified sampled landscapes with Monte Carlo simulations of measured methane emissions in wet and dry soils permitted to integrate landscapes emissions at annual basis with biased uncertainties. Assuming enteric emissions obtained for the Pantanal region, our results suggest that the landscapes methane emissions are 10-to 23-fold superior than the enteric emissions of traditional bovine systems. While enteric emissions seem negligible with respect to net farmland emissions, cattle livestock provide important environmental services like carbon recycling through non-competing herbivory. Moreover, cattle might be making use of a biomass that would undergo decomposition during the flooding phase. Our analysis thus indicate that enteric emissions from traditional bovine systems in flooding farmlands could be considered neutral. By contrast, intensification to improve the stocking rate should be accounted as net anthropogenic emissions. A case study of intensification allowed an increase of 48% in the stocking rate, which is associated with net anthropogenic emissions from 534 bovine animals or about 27 to 63 Mg of enteric CH 4 per year. In short, the
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