With increasing application of silver nanoparticles (AgNPs) in biological sensing, detection, enzyme immunoassays, antimicrobial activity as well as SERS, it has been realised that the toxicity limits their application in biomedicine. However, the interaction of proteins with nanoparticle (NPs) surface possessing different chemical functionalities reduces the potential impact of NPs rendering them biocompatible. Here, we have functionalized the AgNPs with β-hydroxy propyl cyclodextrin (β-HPCD-AgNPs) and studied the conformational changes and thermal stability of haemoglobin (Hb) upon interaction with β-HPCD-AgNPs. Interaction of Hb with borohydride capped AgNPs (BH 4 -AgNPs) was also evaluated. Hb remained in native form upon binding with β-HPCD-AgNPs which is unveiled by UV-vis and fluorescence spectroscopic as well as dynamic light scattering (DLS) studies.However, BH 4 -AgNPs induced unfolding of Hb and reduced the α-helical content following interaction with Hb. Further, the accessibility of tryptophan fluorescence was found to be 33% for β-HPCD-AgNPs quencher, which implies that β-HPCD-AgNPs retains the native conformation of Hb Dichroic study revealed that β-HPCD-AgNPs did not affect thermal stability of Hb, whereas BH 4 -AgNPs decreased the thermal stability of Hb by 5 °C.Haemolysis assay demonstrated the biocompatibility of β-HPCD-AgNPs towards RBC.Functionalization of NPs with controlled surface property thus dictated overall biological reactivity of Hb-AgNPs. Therefore, we trust that the obtained results will help in designing surface functionalized AgNPs for biomedical applications. . Mahato, M.; Pal, P.; Tah, B.; Ghosh, M.; Talapatra, G. B. Study of silver nanoparticle-haemoglobin interaction and composite formation.
Spherical nanoparticles with core-frame architecture are a viable route to combine multiple functionalities on a nanoscopic scale. Amongst these nanoparticles, metal polymeric hybrid nanostructures exhibit significantly enhanced stability. Synergistic catalytic responses arise from quasi perfect morphology and their unique interactions between the metal and reactant substrate. Core-frame silver supported silica nanoparticles (Ag@SiO 2 NPs) with different frame thicknesses were tailored in a controlled manner through an oversimplified environmentally friendly route using simple chemical additives instead of dendrimers as linkers for prior modification of AgNPs. Here the optical and thermal properties of Ag@SiO 2 NPs were studied by high resolution transmission electron microscopy (HRTEM), dynamic light scattering (DLS), Fourier transform infrared spectroscopy (FTIR), and thermogravimetric analysis (TGA).The resulting stabilised nature of Ag@SiO 2 NPs, their functionalization and environmental behaviour were analysed in detail through absorbance measurements. The control over the particle geometry provided an opportunity to utilise this hybrid NP as a temper for faster hydrogenation of p-nitrophenol with minimal reductant concentration (3 mM NaBH 4 ). The effect of the volume ratio of the hybrid catalyst with respect to thermal behaviour and their hydrogenation reaction time, average reaction rate and hybrid reusability were thoroughly investigated. The reported high performance towards faster hydrogenation was completed within 300 s at 25 C and 16 s at 60 C. The synergetic behaviour of core-frame morphology provides faster electron transfer for hydrogenation and enhanced thermal stability against poisonous environments.
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