Highly sensitive detection of proteins using voltammetric assay in the presence of silver nanostructures

Devi, Loganathan Bhavani; Berchmans, Sheela; Mandal, Asit Baran

doi:10.1016/j.jelechem.2011.11.013

Cited by 10 publications

(3 citation statements)

References 48 publications

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“…These cells then enter the bloodstream. Generally, imaging tests such as x-rays are unable to detect leukemia because it does not form into a mass (tumor) [2]. Biomarkers can be used as an early detection method in the form of substances that can be measured.…”

Section: Introductionmentioning

confidence: 99%

Low Albumin Determination as a Biomarker Cancer with Cyclic Voltammetry in Agfilm/ITO and AgNOfilm/ITO Electrodes

Zulfa,

Farahdina,

Aziz

et al. 2023

Trends Sci

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Ag and AgNO film were fabricated through sputter deposition on indium tin oxide (ITO) conductive glass. Electrochemical detection using cyclic voltammetry (CV) was carried out for low albumin levels in blood as a parameter of cancer patients. This study aims to determinate low albumin level as a biomarker cancer. Albumin adsorption on the Agfilm/ITO and AgNOfilm/ITO was also investigated to determine the level of sensitivity of both electrodes. Analysis of the CV measurements indicated that the Agfilm/ITO electrode was more sensitive compared to the AgNOfilm/ITO electrode, with a sensitivity value of 4.564 µA M-1cm-2 for the Agfilm/ITO electrode and 2.123 µA M-1cm-2 for the AgNOfilm/ITO electrode. The testing of albumin levels in blood within a range of low concentration levels between 10-1 and 10-8 g/dL indicated a detection limit of 10-9 g/mL. The selectivity of Ag electrodes was found to be very good for other interfering molecules such as urine, glucose, and lysozyme. The results of the modeling of electric field and magnetic field distribution showed that the Agfilm/ITO electrode possessed larger values than the AgNOfilm/ITO electrode. From this analysis, it can be concluded that the electrode modified with micro-sized Ag achieved more effective results than the electrode modified with AgNO. This sensor can determine low albumin level as a blood cancer biomarker. This sensor holds great promise for use in real samples in the future. HIGHLIGHTS Early detection with albumin biomarkers before metastasis cancer The testing of albumin levels with Cyclic Voltammetry and Electric Impedance Spectroscopy in the cancer within a wide range of low concentration levels (10-1 - 10-8) indicated a detection limit of 10-9 g/mL. The total mass of adsorbed BSA in electrode surface is more higher (281 mgm-2) than previous study. The Agfilm/ITO electrode exhibited high sensitivity and long-term stability for future applications. GRAPHICAL ABSTRACT

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Section: Introductionmentioning

confidence: 99%

Low Albumin Determination as a Biomarker Cancer with Cyclic Voltammetry in Agfilm/ITO and AgNOfilm/ITO Electrodes

Zulfa,

Farahdina,

Aziz

et al. 2023

Trends Sci

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“…[26][27][28] The introduction of some biomolecules as environmental friendly reducing and capping agents for the synthesis of noble metal nanoparticles has been attempted in the past. 29,30 For example, ''green'' synthesis of AuNPs employing b-Dglucose and starch as reducing and capping agent had been carried out. 29 Thus, the potential of cyclodextrins (CDs) as renewable reducing/capping agents towards the synthesis of nanoparticles had been demonstrated.…”

Section: Introductionmentioning

confidence: 99%

Self-assembly of Ag nanoparticles using hydroxypropyl cyclodextrin: synthesis, characterisation and application for the catalytic reduction of p-nitrophenol

Devi

Mandal

2013

RSC Adv.

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“…Bovine serum albumin (BSA) is an interesting protein in analytical biology 1 due to its good bio-receptor as a human antigen recognition system, 2 and identical structure to human serum albumin (HSA). 3 Moreover, the content of BSA in blood and urine is an indicator of the presence of certain diseases, thus BSA detection is urgent in the screening stage to avoid a worse risk.…”

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confidence: 99%

A Spin-Valve GMR Based Sensor with Magnetite@silver Core-Shell Nanoparticles as a Tag for Bovine Serum Albumin Detection

Wibowo

Juharni

Sabarman

et al. 2021

ECS J. Solid State Sci. Technol.

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The availability of rapid-portable instruments to monitor the bovine serum albumin (BSA) concentration is vital in the early detection of disease. In this research, an original biosensing method based on a spin-valve giant magnetoresistance as a transducer and magnetite@silver core-shell magnetic nanoparticles (MNPs) as a tag has been proposed for detecting BSA. The synthesized magnetite@silver MNPs tag size is ≈15 nm with ≈52 emu g −1 of saturation magnetization. This proposed biosensor performs rapid detection of BSA through the shifting of the switching magnetic field and the increasing of the output voltage. The effectivity of the sensor in monitoring the BSA concentration was shown by a strong linear correlation with ≈0.06 mV/(mg/ml) of the sensitivity and ≈0.44 mg ml −1 of the detection limit. Moreover, the measurement result can be acquired rapidly up to 1 min with low external magnetic field assistance. Therefore, this biosensing technique can be promoted as a real-time portable biosensor.

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Highly sensitive detection of proteins using voltammetric assay in the presence of silver nanostructures

Cited by 10 publications

References 48 publications

Low Albumin Determination as a Biomarker Cancer with Cyclic Voltammetry in Agfilm/ITO and AgNOfilm/ITO Electrodes

Low Albumin Determination as a Biomarker Cancer with Cyclic Voltammetry in Agfilm/ITO and AgNOfilm/ITO Electrodes

Self-assembly of Ag nanoparticles using hydroxypropyl cyclodextrin: synthesis, characterisation and application for the catalytic reduction of p-nitrophenol

A Spin-Valve GMR Based Sensor with Magnetite@silver Core-Shell Nanoparticles as a Tag for Bovine Serum Albumin Detection

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