Penelitian ini dilatarbelakangi oleh adanya guru yang masih melakukan proses pembelajaran secara tekstual dengan menggunakan LKS yang masih memiliki keterbatasan. Tujuan penelitian ini adalah untuk mengetahui peningkatan keterampilan proses sains siswa menggunakan perangkat pembelajaran LKS Blended learning berbasis web materi pencemaran lingkungan. Jenis penelitian ini adalah penelitian Research and Development (R&D). Prosedur penelitian mengadaptasi model pengembangan ADDIE. Penelitian dilaksanakan pada semester genap tahun ajaran 2020/2021 di SMPN 3 Menthobi Raya Kabupaten Lamandau pada siswa kelas VII. Peningkatan keterampilan proses sains siswa siswa diukur dengan tes objektif dan dianalisis dengan N-gain. Berdasarkan hasil penelitian dapat disimpulkan bahwa N-gain keterampilan proses sains siswa mencapai rata-rata 0,72 uji skala kecil dan 0,84 uji skala besar. LKS blended learning berbasis web pada materi pencemaran lingkungan dapat meningkatkan keterampilan proses sains siswa.
Jumlah populasi rajungan di wilayah perairan Laut Jawa, Kabupaten Sukamara mulai mengalami penurunan. Terjadinya penangkapan rajungan secara terus-menerus tanpa memperhatikan ekosistem menjadi penyebab utama terhadap penurunan populasi rajungan. Penelitian ini dilakukan untuk mengetaui bioekologi, kelimpahan dan pola distribusi rajungan di perairan laut Jawa, Kabupaten Sukamara. Penelitian ini dilakukan pada bulan Mei sampai Oktober 2019. Pengambilan sampel penelitian dilakukan pada 4 stasiun penelitian menggunakan pukat. Data yang dikumpulkan adalah jumlah individu, lebar rajungan, rasio jenis kelamin, kelimpahan, dan pola distribusi rajungan. Hasil penelitian menunjukkan kelas ukuran lebar rajungan berdasarkan hasil penelitian didominasi oleh rajungan dewasa (> 12 cm). Rasio kelamin rajungan didominasi oleh rajungan jantan. Rajungan betina yang sedang bertelur, terutama pada bulan September-Oktober menunjukkan jumlah peningkatan. Kelimpahan rajungan tertinggi pada stasiun 1 dan terendah pada stasiun 4. Kelimpahan tertinggi di bulan Mei, Juli dan September pada stasiun 1, 2, 3, dan tertinggi di bulan Juni dan Agustus pada stasiun 4.
Mercury pollution is dangerous to health. Previous research was found two potential Gram-negative bacteria for mercury bioremediation, from gold mining in Central Kalimantan, Indonesia. These isolates were identified as Pseudomonas aeruginosa KHY2 and Klebsiella pneumonia KHY3. Mechanisms of mercury bioremediation had not known yet by these isolates. This study purposed to test the role of coenzymes on mercury bioremediation by these isolate and to determine the coenzymes best level of mercury bioremediation. Experimental design was Completely Randomized Design in a laboratory. Treatment factors were coenzymes obtained from vitamins B1, B6, B12, with 6 levels of treatments, included 1 control. All treatments were done in Luria Broth media that contain 12 ppm of mercury. Mercury was measured by AAS Shimadzu AA-6200. The results showed that coenzymes effect was very significant to improve mercury bioremediation by P. aeruginosa KHY2 and K. pneumonia KHY3. Supplementation of vitamin B12 in culture media, more enhance of mercury bioremediation compared with vitamin B1 and B6. These result above, indicated the mechanism of mercury bioremediation in both isolates, were the enzymatic process.
This research was carried out in the oil palm plantation of PT. MuliaSawitAgro Lestari (PT. MSAL) Gunung Mas Regency Central Kalimantan Province for 3 months starting in January 2019 until March 2019. The purpose of this study was to identify the potential of microorganisms as biofertilizers from palm oil mill effluent (PKS). This research is an exploratory study conducted in the Laboratory of the Faculty of Health Sciences of the Muhammadiyah University of Palangka Raya. This study used 3 (three) media used to identify the potential of microorganisms for biofertilizer from palm oil liquid waste by calculating the number of bacterial colonies namely Carboxy Methyl Cellulose (CMC), pykovskaya media and Yeast Extract Mannitol Agar (Yema) media. The results of the analysis also showed that PKS liquid waste contained potential microorganisms found were phosphate solvent bacteria, cellulite fungi and nitrogen-fixing bacteria with an average bacterial colony of 1,700,000 cells/mL. Potential microorganisms from the proportion obtained showed the ability of bacteria found in PKS liquid waste to degrade the oil. The first and second samples of bacterial forms identified were in the form of bacilli and were gram-positive bacteria, while the samples of the three bacteria were in the form of coccus and the bacteria were gram-negative bacteria.
Gold miners use mercury for the gold extraction process, and annually releases no less than 1,000 tons of hazardous materials into the environment. The mercury content in the Kahayan watershed reaches 2,996 - 4,687 g/l and this value exceeds the standard limit for mercury content in water. Mercury is highly toxic so that it causes negative impacts on the environment and public health. Bioremediation is one of solution to reduce mercury waste pollution. Mercury bioremediation using microorganisms and adsorbents requires appropriate process steps to be effective in eliminating mercury. This study aims to determine the effect of the bioremediation steps on the effectiveness of mercury (Hg) elimination in liquid media and to determine the most effective bioremediation steps for mercury (Hg) elimination in liquid media. This study used an experimental method with a non-factorial Completely Randomized Design consisting of treatments, namely control (K), treatment 1 (T1), treatment 2 (T2), treatment 3 (T3), treatment 4 (T4), with 5 (five) times repetition. Analysis the results of the bioremediation stage used AAS (Atomic Absorption Spectrophotometry). Data were analyzed using One-way Anova test, LSD test and effectiveness test. The results showed that the bioremediation stage had a significant effect on the effectiveness of mercury (Hg) elimination in liquid media, this was proven by the results of statistical tests carried out using the One-way Anova test and further tests where the different reduction data obtained from each treatment. The initial level mercury 10 ppm, after being given treatment decreased T1 to 1.25 ppm, T2 to 1.33 ppm, T3 to 1.06 ppm, and T4 to 1.24 ppm. The most effective bioremediation step for the elimination of mercury (Hg) in liquid media is the T3 stage starting from Mercury Waste→Microalgae→Bacteria→Red Clay→Activated Charcoal, with a mercury reduction effectiveness value of 89.4%.
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