The Role of Coenzymes on Mercury (Hg2+) Bioremediation by Isolates Pseudomonas aeruginosa KHY2 and Klebsiella pneumonia KHY3

Abstract

Mercury pollution is dangerous to health. Previous research was found two potential Gram-negative bacteria for mercury bioremediation, from gold mining in Central Kalimantan, Indonesia. These isolates were identified as Pseudomonas aeruginosa KHY2 and Klebsiella pneumonia KHY3. Mechanisms of mercury bioremediation had not known yet by these isolates. This study purposed to test the role of coenzymes on mercury bioremediation by these isolate and to determine the coenzymes best level of mercury bioremediation. Experimental design was Completely Randomized Design in a laboratory. Treatment factors were coenzymes obtained from vitamins B1, B6, B12, with 6 levels of treatments, included 1 control. All treatments were done in Luria Broth media that contain 12 ppm of mercury. Mercury was measured by AAS Shimadzu AA-6200. The results showed that coenzymes effect was very significant to improve mercury bioremediation by P. aeruginosa KHY2 and K. pneumonia KHY3. Supplementation of vitamin B12 in culture media, more enhance of mercury bioremediation compared with vitamin B1 and B6. These result above, indicated the mechanism of mercury bioremediation in both isolates, were the enzymatic process.

Removal efficacy of toxic metals in leachate through micro-organisms isolated from the natural environment

“…Bacteria, such as Pseudomonas sp., Bacillus sp., Acinetobacter sp., Stenotrophomonas sp., Rhodococcus sp., and Burkholderia sp., have been isolated from actively stored soil and leachate in the literature (Imron et al 2019). It is also reported that P. aeruginosa isolated from leachate of solid waste landfills can remove about 29, 30, 35, and 28% of Pb, Cr, Ni, and Cd, respectively, and P. aeruginosa KHY2 and Klebsiella pneumonia KHY3 isolated from gold mine wastewater can reduce 1000 mg L -1 Hg by up to 60% (Neneng and Gunawan 2018).…”

Resistance of bacteria isolated from leachate to heavy metals and the removal of Hg by Pseudomonas aeruginosa strain FZ-2 at different salinity levels in a batch biosorption system

“…P. aeruginosa isolated from solid waste disposal dumpsite leachate can remove approximately 29, 30, 35, and 28% of Pb, Cr, Ni, and Cd, respectively [19]. Neneng and Gunawan [20] reported that P. aeruginosa KHY2 and Klebsiella pneumonia KHY3 isolated from gold mine wastewater exhibited the capability to remove 1000 mg L − 1 of Hg up to 60%. De et al [21] claimed that P. aeruginosa strain CH07 isolated from marine sediments could remove up to 95% of Hg at 8 mg L − 1 of Hg and 8 g L − 1 of NaCl.…”

Действие Двухвалентных Катионов Металлов На Функционирование Надф+-Зависимой Малатдегидрогеназы Из Листьев Кукурузы

“…Одни катионы являются для энзимов необходимыми коферментами[12][13][14], без которых невозможно протекание реакции. Другие, в том числе тяжелые металлы, загрязнение которыми вызывает определенные опасения, ингибируют эти процессы[15, 16].Было исследовано влияние хлоридов ионов Mg 2+ , Мn 2+ , Ва 2+ , Са 2+ на активность очищенного препарата. Показано, что магний в небольших концентрациях (до 3 мМ) активирует НАДФ + -зависимый МДГ (рис.2.).…”