Aklimatisasi merupakan tahap yang kritis bagi plantlet. Pada lingkungan yang baru plantlet dapat mengalami cekaman lingkungan. Kondisi lingkungan seperti media yang tidak mendukung pada tahap aklimatisasi dapat menyebabkan kematian plantlet. Penelitian ini bertujuan: 1) Mendapatkan kombinasi media yang paling baik bagi keberhasilan aklimatisasi plantlet anggrek Phalaenopsis; 2)Mengetahui apakah terdapat interaksi antara media bagian bawah dengan atas pot; 3) Mendapatkan kombinasi media yang paling baik bagi pertumbuhan bibit anggrek anggrek Phalaenopsis pada tahap pembesaran, dan 4) Melihat apakah media sabut kelapa dapat digunakan untuk aklimatisasi dan pembesaran anggrek Phalaenopsis. Penelitian dilaksanakan di rumah jaring Politeknik Negeri Lampung dari bulan Maret hingga Agustus 2018. Plantlet diaklimatisasikan 4 minggu. Percobaan faktorial dilaksanakan dalam rancangan acak kelompok. Faktor A : macam media bagian bawah (bata/arang). Faktor B : macam media bagian atas (moss sphagnum/pakis/sabut kelapa). Enam kombinasi perlakuan dicobakan (masing-masing dengan 10 ulangan), yaitu:  P1 (bata+moss); P2 (bata+pakis); P3 (bata+sabut kelapa); P4 (arang+moss); P5 (arang+pakis); dan P6 (arang+sabut kelapa). Peubah yang diamati: 1) persen keberhasilan plantlet menjadi bibit, 2) tinggi bibit, 3) panjang daun, 4) lebar daun, dan 5) jumlah daun.  Data yang diperoleh dilakukan analisis ragam dan dilanjutkan dengan uji BNJ 5%. Hasil penelitian menunjukkan: 1) Media moss atau pakis dikombinasikan dengan bata maupun arang memberikan persen keberhasilan aklimatisasi plantlet anggrek Phalaenopsis yang paling baik (100%); 2) Tidak terdapat interaksi antara media bagian atas dengan bagian bawah; 3) Kombinasi media yang paling baik bagi pertumbuhan bibit anggrek Phalaenopsis pada tahap pembesaran adalah moss baik dikombinasikan dengan bata ataupun arang, kemudian diikuti oleh pakis (dikombinasikan dengan bata ataupun arang); dan 4) Media sabut kelapa dapat digunakan untuk aklimatisasi dan pembesaran bibit anggrek Phalaenopsis bila dilakukan penyiraman yang lebih intensif.
Banana is one of the most important global agriculture commodities. Asia accounts for the production of bananas amounted to 56.4% of total banana in the world and Indonesia is one of the banana-producing countries in Asia. The demand for bananas increased need is anticipated with good cultivation techniques to meet domestic and international market demand. Plant regeneration in vitro tissue culture techniques or can be used to produce propagules true-to-type in large quantities in a relatively short period and free from disease. This research aims to study the influence of various concentrations of BA (0, 0.5, 1.0, 1.5, 2.0, 2.5, 3.0, 3.5, and 4.0 mg/l) against the growth and reproduction of Cavendish banana propagules in vitro. Time appears buds, propagules length and the number of propagules per explant, and the number of primary roots of plants per explant observed after eight weeks of culture. The experiment results show that after explant grown for eight weeks on the media's treatment shows the different response. The increased concentration of BA can increase the number of shoots. The increased concentration of BA from 0.5 mg/l be 1-3 mg/l increased the number of propagules, but the number of propagules will decrease in the concentration of BA more 3.5 or 4 mg/l. In addition to BA concentration 0.5, 1 and 1.5 mg/l MS media into producing shoot buds appear faster time high, highest propagules and root amount more on banana propagules Cavendish, but the number of shoots is fewer. The best of BA concentrations to induce propagules plant bananas Cavendish is 3.0 mg/l.
The level of fertility of paddy fields in Banjarrejo was decreasing, this is indicated by low rice productivity, fertilization response was leveling off, topsoil was shallow, and rice easy lodged. Fertility degradation of paddy fields occurs continuously. Factors that encourage the ongoing process of soil fertility degradation is a concept application of High External Input Agriculture (HEIA) on the cultivation of crops, especially rice. Rice straw can be used as an alternative to increase soil fertility and maintain the health of paddy field. Straw is the main product of rice cultivation in the form of organic fertilizer because its potential reaches 1.5 x grain yield. However, the potential that is available cheaply and easily and Lack of information and knowledge about straw causes many farmers not to utilize straw to the fullest. Straw is still regarded as an obstacle in tillage, where the nest mice, disease sources, and sources of dirt that must be cleaned. Farmers have not used it to be composted because many still do not have the skills to make it. State Polytechnic of Lampung has the role and responsibility to transfer technology that is beneficial to society especially in the case of making straw compost through the program of Community Service. Technical Training Composting of Rice Straw was conducted in Banjarrejo Village, Batanghari District, East Lampung Regency in April-September 2013. The use of straw compost in paddy fields can increase rice production while maintaining sustainable soil health.
Salah satu upaya dan inovasi dalam penyediaan benih kentang unggul adalah melalui teknik in vitro. Penambahan hormon dan bahan alami lain pada teknik in vitro kentang seperti NAA dan air kelapa dapat menjadi salah satu alternatif dalam perbaikan mutu benih yang dihasilkan. Penelitian ini bertujuan untuk: mengetahui pengaruh perlakuan NAA terhadap multiplikasi tunas kentang atlantik secara in vitro, mengetahui pengaruh perlakuan air kelapa terhadap multiplikasi tunas kentang atlantik secara in vitro, dan mengetahui pengaruh interaksi perlakuan NAA dan air kelapa terhadap multiplikasi tunas kentang atlantik secara in vitro. Penelitian ini dilaksanakan di Laboratorium Kultur Jaringan Politeknik Negeri Lampung pada bulan Oktober 2014 - bulan Januari 2015. Rancangan yang digunakan dalam penelitian ini adalah Rancangan Acak Kelompok (RAK) dengan 2 faktor 3 ulangan. Faktor pertama adalah konsentrasi NAA yang terdiri dari 2 taraf yaitu 0 ml-l dan 1 ml-l. Faktor kedua adalah konsentrasi air kelapa yang terdiri dari 4 taraf yaitu 0 ml-l, 100 ml-l, 150 ml-l dan 200 ml-l. Dari hasil analisis data secara diperoleh bahwa perlakuan NAA dan air kelapa berpengaruh nyata terhadap jumlah hari pembentukan tunas (hari), jumlah hari pembentukan akar (hari) dan panjang tunas per eksplan (cm). Interaksi keduanya berpengaruh nyata terhadap parameter jumlah hari pembentukan akar (hari) dan jumlah tunas cabang primer per eksplan.
Efforts to increase the quality of Dendrobium orchids can be made by increasing the genetic quality through crosses while increasing the quantity can be done by propagation through in vitro culture. Information about the compatibility of an orchid species is very important to maximize the acquisition of seeds as propagation material. The seeds from the cross were propagated by in vitro culture technique. The purpose of this study was to obtain a compatible crossing method between crossing and selfing D.Polinela Lampung orchids with selected parents and to determine the effect of giving a combination of media with coconut water concentration in inducing germination and growth of orchid seeds. This study consisted of two experiments, namely (1) the compatibility test of D.Polinela Lampung orchids with three types of Indonesian orchids (D.discolour, D.leporinum, D.lasientera), and (2) the effect of basic media (MS, VW, Growmore, Gandasil) and water concentration CW (0, 50, 150, and 250 ml/1) on seedling growth of D.Polinela Lampung x D.lasientera orchids. In conclusion, among the sixteen crosses of Dendrobium orchids, ten compatibility crosses were found (pollination that produces fruit). The addition of 15 and 25% CW in the growmore medium (91.37% and 98.03%), MS (90.97% and 96.00 %), and Gandasil (90.67% and 92.67%) gave the highest percentage results. Seed germination and protocorm formation. The protocorm that forms the first leaves produced on Growmore media added with 50-150 ml/1 coconut water gave the most results compared to other treatments. The results of the research are expected as basic information in orchid propagation, especially the study of compatibility and germination of orchid seeds.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.