Multiplikasi Mata Tunas Pisang ‘Cavendish’ In Vitro Pada Berbagai Konsentrasi Benziladenin

Abstract

Banana is one of the most important global agriculture commodities. Asia accounts for the production of bananas amounted to 56.4% of total banana in the world and Indonesia is one of the banana-producing countries in Asia. The demand for bananas increased need is anticipated with good cultivation techniques to meet domestic and international market demand. Plant regeneration in vitro tissue culture techniques or can be used to produce propagules true-to-type in large quantities in a relatively short period and free from disease. This research aims to study the influence of various concentrations of BA (0, 0.5, 1.0, 1.5, 2.0, 2.5, 3.0, 3.5, and 4.0 mg/l) against the growth and reproduction of Cavendish banana propagules in vitro. Time appears buds, propagules length and the number of propagules per explant, and the number of primary roots of plants per explant observed after eight weeks of culture. The experiment results show that after explant grown for eight weeks on the media's treatment shows the different response. The increased concentration of BA can increase the number of shoots. The increased concentration of BA from 0.5 mg/l be 1-3 mg/l increased the number of propagules, but the number of propagules will decrease in the concentration of BA more 3.5 or 4 mg/l. In addition to BA concentration 0.5, 1 and 1.5 mg/l MS media into producing shoot buds appear faster time high, highest propagules and root amount more on banana propagules Cavendish, but the number of shoots is fewer. The best of BA concentrations to induce propagules plant bananas Cavendish is 3.0 mg/l.

Micropropagation of Banana Plant Cavendish (Musa acuminata L) Using Shoot-tip Culture to Support Food Security

“…Giant Cavendish 5.0 mg/L BAP + 0.5 mg/L NAA (Gebeyehu, 2015), multiple Musa sapientum shoots were induced in vitro from shoot meristems with Murashige and Skoog's medium supplemented with BAP 3.0 mg/L and NAA 0.2 mg/L (Kalimuthu et al, 2007). The best BAP concentration to induce propagules plant bananas Cavendish is 3.0 mg/L (Maulida et al, 2018), whereas using a low BAP combination of 0.2 mg/L + IAA, 0.1 mg/L in Musa paradisiaca L also resulted in a low number of shoots (Dhanalakshmi & Stephan, 2016) and the same thing for Kepok Amorang Banana shoot multiplication using ¼ MS + 1 ppm BAP, the rate of shoot multiplication obtained in this study was low, which ranged from 1-3 (Supriati, 2010). BAP primarily influences the development of explants, namely in the formation of shoots, shoot multiplication, and spurring cell division in plant metabolism to form the necessary organs (Faridah et al, 2017).…”

Meningkatkan Pendapatan Petani melalui Pendampingan Budidaya Pisang Cavendish di Desa Sukadana

“…Pohon pisang cavendish merupakan jenis pisang yang paling komersil dipasar internasional. Pisang cavendish dapat dibudidayakan di kawasan tropis dan subtropis (Maulida et al, 2018). Peluang pembudidayaan pisang cavendish di Indonesia sangat potensial untuk dikembangkan petani lokal perorangan maupun perusahaan (Muktianto & Indriyani, 2022) .…”

Respons Pertumbuhan Tiga Varietas Pisang Lokal Terhadap ZPT Benzil Adenin (Ba) Secara in Vitro

“…Berdasarkan uraian di atas dapat diketahui bahwa jenis pisang dan konsentrasi BA dapat menyebabkan perbedaan respons dari eksplan untuk menghasilkan tunas. Maulida et al (2018) menyebutkan bahwa kebutuhan jenis dan konsentrasi sitokinin untuk merangsang pembentukan tunas bersifat spesiesspesifik, artinya bahwa konsentrasi sitokinin untuk setiap jenis pisang berbeda. Selanjutnya Bella et al (2016) menyatakan bahwa perbedaan jumlah tunas yang dihasilkan setiap jenis pisang dapat dipengaruhi oleh genotipe tanaman atau kemampuan eksplan dalam menyerap unsur hara yang terdapat dalam media kultur.…”

“…Gambar 4. Rata-rata jumlah akar dari tiga jenis pisang pada media kultur jaringan (sumber: data hasil penelitian diolah) Maulida et al (2018) menunjukkan bahwa peningkatan konsentrasi BA pada eksplan pisang Barangan umumnya menghasilkan tunas tanpa akar, sementara jumlah akar ekslpan paling banyak dihasilkan oleh eksplan dengan penambahan BA 0.5 mg/l. Hal ini seperti yang dinyatakan oleh Mangena (2020) bahwa benziladenin dapat menghambat pertumbuhan akar adventif.…”