Abstract. Suhartati L, Khaerunnisa L, Gunawan A, Rukmiasih, Darwati S, Sumantri C, Rizqan. 2020. Short Communication: Identification of the exon 1 myostatin gene polymorphism and its association with slaughtered weight in Indonesian Kampung and Broiler Chicken. Biodiversitas 21: 3893-3897. Myostatin is widely known as a negative regulator of skeletal muscle development by controlling hypertrophy and hyperplasia. Silent mutation in exon 1 (c.234G>A) was then thought to be associated with chicken growth performances. This study was designed to analyze polymorphisms of c.234G>A mutation of the myostatin gene and its effect on slaughtered weight in Indonesian Kampung and meat-type chicken. The SatI restriction enzyme was used for genotype determination through the PCR-RFLP technique. The effect of genotype on the slaughtered weight was analyzed using the General Linear Model (GLM) procedure. Genotyping was performed on 138 chicken from Kampung (n=67) and meat type (cobb Broiler) (n=71) chicken. The amplification product contained 226 bp nucleotides. This myostatin gene was polymorphic in both chicken population, produced 2 alleles (G and A), and 3 genotypes (GG, GA, and AA). The A allele had a higher frequency than the G allele in all populations. In this study, genotype of AA and GA had higher live weight, carcass weight, breast weight, thigh weight, drumsticks weight, wings weight, breast muscle weight, thighs muscle weight, drumsticks muscle weight than GG genotype of 12 weeks-old Kampung chicken. The results suggested that the c.234G>A mutation in exon 1 could be potentially recommended as a genetic marker for chicken slaughtered weight traits.
This study was conducted to determine the performance of male Kamang ducks reared with pools and without pools with three different levels of ration energy. This study used a Randomised Complete Block Design (RCBD) with a divided plot design with the main plot consisting of pools, and without pools, subplots consisted of ration energy levels of E1 (2700 Kcal/kg), E2 (2900 Kcal/kg) and E3 (3100 Kcal/kg) with three groups. The study raised 90 male Kamang ducks. The treatment began at two weeks of age and ended at the 10 th week Each cage consisted of 5 Kamang ducks per treatment making a total of 90 ducks. The variables observed were ration consumption, body weight gain, feed conversion, and growth rate. The results showed that there was is no interaction between the duck rearing method with varying ration energy levels on ration consumption, body weight gain, ration conversion, and growth rate (p>0.05). Also, the level of ration energy did not affect ration consumption, body weight gain, ration conversion, and growth rate (P>0.05). However, the maintenance of ducks with and without pools was significantly different (p<0.05) with the average body weight gain and feed conversion. The study concludes that rearing ducks using ponds showed the best results at 2700 Kcal/kg (E1) energy level with an average ration consumption of 5907.7 g/duck, weight gain of 1232. 51 g/duck, and feed conversion ratio of 4.82.
The purpose of this study was to increase the nutritional content of brown seaweed type Turbinaria murayana by using fermentation methods using different local microorganisms (MOL) as ingredients for poultry feed. The material used is brown seaweed type Turbinaria murayana taken from Sungai Nipah Beach, Pesisir Selatan Regency, West Sumatra Province and MOL as an inoculum. The study used a completely randomized design (CRD) with six treatments and four replications. The treatment was fermentation using different MOLs, namely No MOL (Control), Bamboo Shoot MOL, Rice MOL, Fruit MOL, Vegetable MOL and Banana Weevil MOL. The results showed that fermentation of brown seaweed type Turbinaria murayana using different MOL had a very significant effect (P≤0.01) on the levels of dry matter, crude fibre, crude protein and crude fat. The best improvement in the nutritional quality of fermented Turbinaria murayana seaweed was found in the fermentation treatment using fruit MOL with the nutritional content of 95.17% dry matter, 5.27% crude fiber, 21.43% crude protein, and 2.41% crude fat.
Penelitian ini bertujuan untuk mengetahui pengaruh pengolahan rumput laut Turbinaria murayana (Phaeophyceae) dengan teknologi fermentasi menggunakan Mikroorganisme Lokal (MOL) berbeda sebagai bahan pakan unggas. Materi yang digunakan yaitu rumput laut jenis Turbinaria murayana yang diambil dari Pantai Sungai Nipah Kabupaten Pesisir Selatan dan MOL sebagai inokulum. Penelitian menggunakan Rancangan Acak Lengkap (RAL) dengan 6 perlakuan, masing-masing perlakuan diulang 4 kali. Perlakuan berupa fermentasi menggunakan MOL berbeda yaitu tanpa MOL (kontrol), MOL rebung, MOL nasi, MOL buah, MOL sayur, dan MOL bonggol pisang. Data dianalisis dengan menggunakan analisis sidik ragam, Jika terdapat perbedaan antar perlakuan, diuji dengan Duncan Multiple Range Test (DMRT). Hasil penelitian menunjukkan bahwa fermentasi Turbinaria murayana menggunakan MOL berbeda berpengaruh sangat nyata (P?0,01) terhadap bahan kering, serat kasar, protein kasar, dan berpengaruh tidak nyata (P?0,05) terhadap lemak kasar. Pengolahan Turbinaria murayana dengan Teknologi fermentasi menggunakan MOL berbeda yang terbaik terdapat pada perlakuan menggunakan MOL buah dengan kandungan nutrien 93,76% bahan kering, 9,46% serat kasar, 22,56% protein kasar, dan 1,53% lemak kasar.
Probiotics are live microorganisms that can improve the health of humans or livestock by balancing the microflora in the digestive tract when consumed in sufficient quantities. Purple sweet potato has high effectiveness against LAB growth, namely 10,771 (log10 cfu/g) with 85.34% resistance be used as a medium for LAB growth. This study aims to determine the effect of probiotics Lactococcus plantarum and Pediococcus pentasaceus using a purple sweet potato carrier on laying hen's performance and egg quality. This study used 210 layers of medium-type laying hens, Strain Isa Brown, 38 weeks old. The method used was an experiment with a completely randomized design (CRD) with seven treatments and three replications. The observed variables were ration consumption, ration conversion, egg mass production, hen day production (HDP), egg weight, shell thickness, and egg cholesterol. The results showed that supplementation of probiotics Lactococcus plantarum and Pediococcus pentasaceus using a purple sweet potato carrier at a dose of 2 grams could affect daily egg production, egg mass and reduce the value of ration conversion, shell thickness and egg cholesterol, but doses of 1 gram and 2 grams did not affect ration consumption and egg weight. It was concluded that the administration of the best LAB probiotics Lactococcus plantarum and Pediococcus pentasaceus with a dose of 2 grams.
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