− Seven flavonoid compounds, kaempferol (1), quercetin (2), quercetin (6) and quercetin-3-O-rutinoside (7), were isolated from the methanolic extract of leaves of Kalanchoe prolifera. Compounds 1-7 were isolated for first time from this plant. These compounds were evaluated their cytotoxic activity against P-388 murine leukimia cells in vitro. Among those compounds kaempferol (1) and quercetin (2) showed strongest cytotoxic activity with IC 50 values of 4.45 ± 0.05 and 6.28 ± 0.02 μg/mL, respectively.

Flavonoids compounds such as kaempferol (1), kaempferol-3-O--D-glucopyranoside or astragalin (2) and kaempferol-3-O--L-rhamnoside or afzelin (3) have been isolated from the fresh leaves of Kalanchoe tomentosa (Crassulaceae). The chemical structure of isolated compounds 1-3 were identified by spectroscopic evidences and comparison with those compound previously reported. Compounds 1-3 showed cytotoxic activity against P-388 murine leukimia cells with IC 50 values of 51.8, 100 and 3.32 μg/mL, respectively.

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Kalanchoe plant, known as "sosor bebek" in Indonesia is a perennial herb and has succulent leaves. The plant is known in folklore and traditional medicine in Indonesia for the treatment of fever, abscesses, bruises, contused wounds, coughs and skin diseases. During the course of our continuing search for novel cytotoxic compounds, the methanolic extract of Kalanchoe tomentosa plants showed cytotoxic activity against P-388 murine leukemia cells. The methanolic extract of the fresh leaves of K. tomentosa was concentrated and extracted successively with n-hexane, ethyl acetate and n-butanol. The ethyl acetate extract exhibited strongest cytotoxic activity againts P-388 murine leukemia cells. By using the cytotoxic activity to follow the separation, the ethyl acetate fraction was separated by combination of column chromatography on silica gel and preparative TLC on silica gel GF 254 to afford a kaempferol-3-O-glycosides (1) and kaempferol-3-O-rhamnoside (2). Compound 1 and 2 showed cytotoxic activity against P-388 murine leukemia cells with IC 50 > 100μg/mL and IC 50 3.32 μg / mL.

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