Pitanga, a fruit of the pitangueira tree (Eugenia uniflora L.), is native to Brazil and has a high antioxidant capacity due to the elevated amount of anthocyanins. The present study aimed to investigate the chemical composition of the purple pitanga fruit and to evaluate its antioxidant effect in the nematode Caenorhabditis elegans. We observed that the ethanolic extract of purple pitanga did not cause any toxic effects but notably increased worm lifespan. The extract improved the survival, reproduction and lifespan of the worms in pre- and post-exposure to stressors HO and juglone, as well as improved the lifespan of the oxidative stress hypersensitive strain mev-1. Notably, PPE extract decreased reactive oxygen species by DCF-DA probe and protein carbonyl content from worms stressed with HO. The extract also affected the expression of superoxide dismutase SOD-3 and heat shock protein HSP-16.2 levels, daf 16 target genes that modulate lifespan and antioxidant metabolism. In addition, we demonstrate that these effects are dependent on DAF-16, as PPE extract did not provide protection in daf-16 mutants. Therefore, these results suggest that PPE significantly protected against oxidative stress modulating daf-16 target genes.

Background: Jaboticaba (Myrciaria trunciflora) belongs to the family Myrtaceae and is a fruit native to the Atlantic Forest of Brazil. The aim of this study was evaluate the activity of the extract of jaboticaba against different stresses in Caenorhabditis elegans. Methods: Initially, the toxicological profile of the extract was analyzed, evaluating survival, reproduction, and longevity. After the tests of resistance to oxidative stress, thermal and against ultraviolet radiation were carried out. In order to discover a signaling pathway involved in this process the DAF-16 pathway was analyzed. Results: It was found that the extract has no toxicity and is still able to prolong longevity in concentrations of 50 and 100 μg of GAE/mL. It was able to protect against damage from the juglone, temperature and UV radiation. This effect is mediated by the activation of the translocation of the DAF-16 factor to the cell nucleus and subsequent activation of defense pathways. Conclusions: Together, it is possible to verify that the extract of jaboticaba is important for the protection of the damages to which were daily exposed, in order to decrease the free radicals and consequently the damages that lead to aging and/or the appearance of diseases.[a] J.

No abstract

No abstract

Este estudo tem a proposta de salientar a importância do farmacêutico no âmbito da farmácia hospitalar veterinária. Os autores basearam-se em uma extensa revisão da legislação brasileira e suas respectivas resoluções, bibliografias de cunho científico além de suas respectivas experiências laborais no Hospital Veterinário Universitário de Santa Maria (HVU). Considerando que a competência do farmacêutico tem como enfoque a saúde humana, falta-lhe maior conhecimento da área veterinária, principalmente a nível hospitalar, ponderando todos os aspectos de cunho animal e o processo de gestão de uma farmácia hospitalar. Com isso, a participação de outros profissionais da área veterinária torna-se essencial para atingir a excelência no serviço prestado. Contudo, o farmacêutico ainda é o especialista mais habilitado para no que se refere à atenção farmacêutica, manutenção, manipulação, dispensação e uso correto do medicamento assim como gestor de uma farmácia hospitalar veterinária. Assim, este profissional é fundamental para garantir a saúde e bem-estar animal, trabalhando junto com o corpo técnico neste ambiente para proporcionar um serviço de qualidade.