Involvement of the DAF‐16/FOXO Pathway in the Antioxidant Activity of the Jaboticaba (Myrciaria trunciflora) Extract against Various Stressors Using Caenorhabditis elegans

Abstract

Background: Jaboticaba (Myrciaria trunciflora) belongs to the family Myrtaceae and is a fruit native to the Atlantic Forest of Brazil. The aim of this study was evaluate the activity of the extract of jaboticaba against different stresses in Caenorhabditis elegans. Methods: Initially, the toxicological profile of the extract was analyzed, evaluating survival, reproduction, and longevity. After the tests of resistance to oxidative stress, thermal and against ultraviolet radiation were carried out. In order to discover a signaling pathway involved in this process the DAF-16 pathway was analyzed. Results: It was found that the extract has no toxicity and is still able to prolong longevity in concentrations of 50 and 100 μg of GAE/mL. It was able to protect against damage from the juglone, temperature and UV radiation. This effect is mediated by the activation of the translocation of the DAF-16 factor to the cell nucleus and subsequent activation of defense pathways. Conclusions: Together, it is possible to verify that the extract of jaboticaba is important for the protection of the damages to which were daily exposed, in order to decrease the free radicals and consequently the damages that lead to aging and/or the appearance of diseases.[a] J.

Nicotine-mediated therapy for Parkinson’s disease in transgenic Caenorhabditis elegans model

“…The naphthoquinone juglone is one of the most abundant naphthoquinones, belongs to the quinone class, and produces hydrogen peroxide and intracellular superoxide. As a result, biological macromolecules are damaged, and the aging process and diseases are accelerated ( Benites et al, 2018 ; Ramos Boldori et al, 2023 ). Our worms were treated with Juglone, an intracellular ROS generator, to validate nicotine’s effect against oxidative stress.…”