This protocol describes a rapid DNA and RNA extraction method for plant tissues.Hexadecyltrimethylammonium bromide (CTAB), sodium chloride (NaCl), tris base, and ethylenediaminetetraacetic acid (EDTA) are the main components of the extraction buffer. In contrast to all previously reported protocols, this extraction method does not require any stock solutions. This isolation buffer is potential of extracting both DNA and RNA simultaneously. Depending on the purpose of the project, the corresponding steps can be slightly altered to obtain either DNA or RNA.The big advantage of this method is to use general laboratory chemicals to make a powerful extraction buffer, resulting in high quality and quantity nucleic acid. Also, CTAB in this buffer is capable of isolating nucleic acid from recalcitrant plants enriched in secondary metabolites.Importantly, this method is recommended for the projects at which isolating nucleic acid in a short time is of crucial importance. This method probably is usable for all plant tissues and takes about an hour.
This protocol describes a rapid DNA and RNA extraction method for plant tissues. Hexadecyltrimethylammonium bromide (CTAB), sodium chloride (NaCl), tris base, and ethylenediaminetetraacetic acid (EDTA) are the main components of the extraction buffer. In contrast to all previously reported protocols, this extraction method does not require any stock solutions. This isolation buffer is potential of extracting both DNA and RNA simultaneously. Depending on the purpose of the project, the corresponding steps can be slightly altered to obtain either DNA or RNA. The big advantage of this method is to use general laboratory chemicals to make a powerful extraction buffer, resulting in high quality and quantity nucleic acid. Also, CTAB in this buffer is capable of isolating nucleic acid from recalcitrant plants enriched in secondary metabolites. Importantly, this method is recommended for the projects at which isolating nucleic acid in a short time is of crucial importance. This method probably is usable for all plant tissues and takes about an hour.
SummaryThe evaluation of sugar beet genotypes under different climate conditions is a principal goal of breeding programs. In most studies, environment has a high influence on the qualitative and quantitative traits of sugar beet. Therefore, data collected from different environments may contribute to more accurate genotype selection. In this study, the effect of different environments on sugar beet genotypes’ performance was evaluated using a meta-analysis method based on Hedges’ technique. Data were collected from 149 trials conducted in twelve regions in Iran over 15 years (2003–18). For all trials, the value of the traits was weighted, and subsequently the effect size, reaction ratio and confidence interval were estimated. Among the studied environments, Khoy had a positive effect on root yield, sugar content, sugar yield and white sugar yield. As could be expected, the effect of environment on final yield formation was high, so that the Shiraz environment had a negative effect on root yield and sugar yield. Overall, the ranking of environments based on the meta-analysis results was quite different from that obtained by comparison of mean results.
Nowadays, studies about yew tree as a main source of taxol (anticancer drugs) is being expanded rapidly. In the last few years, taxol is being isolated from the bark of Taxus brevifolia as the species of yew tree, containing very low amount (approximately 0.01%) .Taxol is diterpenoid amide and can be produced by different approaches, suspended cell culture is a method through which taxol can be produced. In this study, 2,4-dichlorophenoxy acetic acid(2,4-D) was nano niosomated in order to deliver the plant hormone to the cells under cultivation. The result demonstrated that the amount of taxol was significantly increased when the cell culture was exposed to pegylated nano niosomal 2 , 4dichlorophenoxy acetic acid(2,4-D) .
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