Performance of Bacillus subtilis D3d xylanase separated through optimized aqueous two-phase system in bio-bleaching of sugar beet pulp

Moteshafi, Hadis; Jabbari, Leila; Hashemi, Maryam

doi:10.1016/j.psep.2022.01.030

Cited by 5 publications

(3 citation statements)

References 39 publications

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“…The location of the critical point of the ternary systems was estimated by extrapolation of the TL compositions applying Eqn (7):…”

Section: Phase Diagramsmentioning

confidence: 99%

“…In addition, ATPSs provide rapid separation, little denaturation, mild operating conditions, rapid mass transfer (low interfacial tension), selective separation (affinity partition) and easy scale-up. 4,5 In this way, different hydrophilic molecules were extracted, concentrated or purified, such as proteins and enzymes, 6,7 alkaloids, 8,9 anthocyanins, 10,11 biomarkers 12,13 and antibiotics, 14,15 among others. However, when applied to ATPSs, hydrophobic molecules migrate to the phase rich in the constituent with a higher hydrophobic characteristic or precipitate at the interface due to their incompatibility with the high water content in both phases.…”

Section: Introductionmentioning

confidence: 99%

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Water‐free two‐phase systems: phase equilibria and partitioning of vitamins from tomatoes (Solanum lycopersicum)

Lima,

Buarque,

Pinto

et al. 2023

J of Chemical Tech & Biotech

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BACKGROUNDThe present work provides diagrams for water‐free two‐phase systems based on polypropylene glycol 2000 (PPG 2000) + [C2mim][OAc] + alcohols (primary: methanol, ethanol, 1‐propanol, and 1‐butanol; secondary: 2‐propanol and 2‐butanol; and tertiary: 2‐methyl‐2‐propanol.) at 298.15 ± 1.00 K and 0.10 ± 0.01 MPa. As a proof of concept, the systems were applied to study the selective partitioning of vitamins, namely β‐carotene, and α‐tocopherol.RESULTSThe hydrophilic/hydrophobic balance is the driving force for phase separation. The more hydrophilic alcohols are responsible for the wider biphasic area, and branching increases hydrophilicity and the biphasic area compared to linear alcohols. Additionally, it was verified that the mathematical determination of the tie‐lines satisfactorily describes the positioning in the phase diagram when compared with the analytical determination. In the proposed systems, pure biomolecules partitioned selectively, α‐tocopherol migrated to the PPG 2000 phase (top phase) while β‐carotene to [C2mim][OAc] phase (bottom phase). For the study with real biomolecules, solid‐liquid extraction was performed using 1‐butanol (more selective alcohol) in a solid‐liquid ratio of 1:10 at 200 rpm for 60 min. The partitioning trend followed the behavior of systems using pure biomolecules. The highest selectivity (5.3) was observed for the system composed of PPG 2000 + [C2mim][OAc] + 1‐butanol or 2‐butanol. The partition system did not impair the antioxidant potential expressed in DPPH inhibition.CONCLUSIONSWater‐free two‐phase systems can be formed and applied to partitioning vitamins from tomatoes.This article is protected by copyright. All rights reserved.

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“…The location of the critical point of the ternary systems was estimated by extrapolation of the TL compositions applying Eqn (7):…”

Section: Phase Diagramsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Water‐free two‐phase systems: phase equilibria and partitioning of vitamins from tomatoes (Solanum lycopersicum)

Lima,

Buarque,

Pinto

et al. 2023

J of Chemical Tech & Biotech

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“…Endo-β-1, 4-xylanase can be derived from microorganisms or plants. Studies have found that some endo-β-1, 4-xylanase derived from Bacillus subtilis (Moteshafia, 2022) and Paenibacillus terrae (Kim, 2016) contain inhibitory proteins, which can reduce the degradation effect of the enzyme. In the study of plant-derived endo-β-1,4-xylanase, there were more research on barley, oat, and rye, but fewer research on wheat.…”

Section: Introductionmentioning

confidence: 99%

Effects of Wheat Malt-Derived Endo-Β-1, 4-Xylanase Supplementation on The Quality of Wheat Beer

Xing

Gao²,

Jin

et al. 2023

BIO Web Conf.

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Endo-β-1, 4-xylanase (EC 3.2.1.8) is a key enzyme in the degradation of Arabinoxylan (AX). AX and its degradation products are the key substances that affect the viscosity, turbidity, filtration speed, alcohol, foam performance, and health care function of wheat beer. A wheat malt-derived endo-β-1, 4-xylanase was isolated and purified by our group in the previous stage. The aim of this study was to investigate the effects of the wheat malt-derived endo-β-1, 4-xylanase addition on the quality and composition of AX in hopped wort and wheat beer. The results showed that the content and the average degree of polymerization (avDP) of water-soluble arabinoxylan (WEAX) in hopped wort and wheat beer decreased with the increase of the addition level of the wheat malt-derived endo-β-1,4-xylanase. When the addition of wheat malt-derived endo-β-1,4-xylanase was 2.5 times of the activity of endo-β-1,4-xylanase in raw malt, the degradation rate of WEAX in hopped wort was the maximum (33.71%), and the avDP of WEAX was the minimum (29.74) at the addition level of the endo-β-1,4-xylanase was 2 times. The viscosity and turbidity of hopped wort and wheat beer decreased continuously with the increase of the addition level of the endo-β-1,4-xylanase, while the foam stability of wheat beer increased continuously. Wheat malt-derived endo-β-1,4-xylanase could decrease the content of 1320-6200 Da polysaccharide in wheat beer, but increase the content of<1320 Da polysaccharide.

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An integrated approach for pulp biobleaching: application of cocktail of enzymes

Angural

Jassal

Warmoota

et al. 2023

Environ Sci Pollut Res

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Performance of Bacillus subtilis D3d xylanase separated through optimized aqueous two-phase system in bio-bleaching of sugar beet pulp

Cited by 5 publications

References 39 publications

Water‐free two‐phase systems: phase equilibria and partitioning of vitamins from tomatoes (Solanum lycopersicum)

Water‐free two‐phase systems: phase equilibria and partitioning of vitamins from tomatoes (Solanum lycopersicum)

Effects of Wheat Malt-Derived Endo-Β-1, 4-Xylanase Supplementation on The Quality of Wheat Beer

An integrated approach for pulp biobleaching: application of cocktail of enzymes

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