Endogenous bacterial strains possessing a high bisphenol A (BPA)-tolerance/degradation activity were isolated from different outlets of petrochemical wastewater in Iran using the enrichment cultivation approach.

A splice form of prochymosin lacking exon‐6 was subcloned and expressed. Expression was optimized by changing various parameters. The maximum amount of protein expressed by this method was 44.61% of total cellular protein, compared to the 45.4% predicted by the Qualitek‐4 software. The expressed protein was then purified using ion exchange chromatography. Activation of the recombinant prochymosin was carried out by changing different parameters. Optimal conditions for activation involved reduction of pH to 5 and 4 h of incubation with the acidic solution, followed by neutralization and 2 h of incubation under neutralized conditions. The activity of the subsequent enzyme sample was 26 units/mL.

Nowadays, hospital-acquired infections caused by multidrug resistant bacterial strains are of great concern. Thus, the development of alternative natural antimicrobial compounds is more necessary than ever. Walnut (Juglans regia L,) leaves are an easily available source of natural compounds with antioxidant and antimicrobial properties. Due to these particular features, we evaluated the antimicrobial effect of the walnut. leaf methanolic extract at three different concentrations (30, 50 and 100 mg/L) against the two clinical strains Pseudomonas aeruginosa and Escherichia coli using the disc diffusion method. The results showed that both clinical strains were sensitive to all the extract concentrations tested. However, the diameter of the inhibition zone corresponding to 100 mg/L of extract concentration for P. aeruginosa was smaller than that for E. coli. These results demonstrate the potential of walnut leaves as an economical alternative source of antimicrobial agents.

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The efficient biodegradation of bisphenol A (BPA) and phenol in salty petrochemical wastewater using a novel indigenous halotolerant, Pseudomonas sp. The bacterium has potential to be used for petrochemical and similar wastewaters treatment.