Abstract:Endogenous bacterial strains possessing a high bisphenol A (BPA)-tolerance/degradation activity were isolated from different outlets of petrochemical wastewater in Iran using the enrichment cultivation approach.
“…Overall, these results showed, that the most suitable BPA concentration for its effective degradation is up to 10 mg L − 1 in rich medium at pH of 7 or 8. This is in agreement with previous studies showing that medium composition may play an important role in complete BPA removal (Badiefar et al 2015 ). Fig.…”
Section: Resultssupporting
confidence: 94%
“…Final concentration of BPA and ethanol in medium were 100 mg L − 1 and 1% (v/v), respectively. Basal Salt Medium (BSM) (Badiefar et al 2015 ) was used for isolation and purification of bacterial species and the degradation study. For bacteria isolation, BSM was supplemented with nystatin (4 g L − 1 ) and actidione (4 g L − 1 ) to inhibit the growth of fungi.…”
Bisphenol A (BPA) is an endocrine disrupting chemical. Its extensive use has led to the wide occurrence of BPA in various environmental ecosystems, at levels that may cause negative effects to the ecosystem and public health. Although there are many bacteria able to BPA utilization, only a few of them have a strong capacity for its biodegradation. Therefore, it is important to search for new bacteria strains, investigate their BPA biodegradation ability and potential effect of pH and other organic compounds on the process. These tasks have become the object of the present study. The results of our research show that for the newly isolated strains Acinetobacter sp. K1MN and Pseudomonas sp. BG12 after 15 days, with an initial BPA concentration of 100 mg L− 1, the highest BPA removal was achieved at pH 8, while sodium glutamate as a biostimulant best accelerated BPA degradation. Kinetic data for BPA biodegradation by both strains best fitted the Monod model. The specific degradation rate and the half saturation constant were estimated respectively as 8.75 mg L− 1 day− 1 and 111.27 mg L− 1 for Acinetobacter sp. K1MN, and 8.6 mg L− 1 day− 1 and 135.79 mg L− 1 for Pseudomonas sp. BG12. The half-maximal effective concentration (EC50) of BPA for Acinetobacter sp. K1MN was 120 mg L− 1 and for Pseudomonas sp. BG12 it was 123 mg L− 1. The toxicity bioassay (Microtox test) showed that elimination of BPA by both strains is accompanied by reduction of its toxic effect. The ability of tested strains to degrade BPA combined with their high resistance to this xenobiotic indicates that Acinetobacter sp. K1MN and Pseudomonas sp. BG12 are potential tools for BPA removal during wastewater treatment plant.
“…Overall, these results showed, that the most suitable BPA concentration for its effective degradation is up to 10 mg L − 1 in rich medium at pH of 7 or 8. This is in agreement with previous studies showing that medium composition may play an important role in complete BPA removal (Badiefar et al 2015 ). Fig.…”
Section: Resultssupporting
confidence: 94%
“…Final concentration of BPA and ethanol in medium were 100 mg L − 1 and 1% (v/v), respectively. Basal Salt Medium (BSM) (Badiefar et al 2015 ) was used for isolation and purification of bacterial species and the degradation study. For bacteria isolation, BSM was supplemented with nystatin (4 g L − 1 ) and actidione (4 g L − 1 ) to inhibit the growth of fungi.…”
Bisphenol A (BPA) is an endocrine disrupting chemical. Its extensive use has led to the wide occurrence of BPA in various environmental ecosystems, at levels that may cause negative effects to the ecosystem and public health. Although there are many bacteria able to BPA utilization, only a few of them have a strong capacity for its biodegradation. Therefore, it is important to search for new bacteria strains, investigate their BPA biodegradation ability and potential effect of pH and other organic compounds on the process. These tasks have become the object of the present study. The results of our research show that for the newly isolated strains Acinetobacter sp. K1MN and Pseudomonas sp. BG12 after 15 days, with an initial BPA concentration of 100 mg L− 1, the highest BPA removal was achieved at pH 8, while sodium glutamate as a biostimulant best accelerated BPA degradation. Kinetic data for BPA biodegradation by both strains best fitted the Monod model. The specific degradation rate and the half saturation constant were estimated respectively as 8.75 mg L− 1 day− 1 and 111.27 mg L− 1 for Acinetobacter sp. K1MN, and 8.6 mg L− 1 day− 1 and 135.79 mg L− 1 for Pseudomonas sp. BG12. The half-maximal effective concentration (EC50) of BPA for Acinetobacter sp. K1MN was 120 mg L− 1 and for Pseudomonas sp. BG12 it was 123 mg L− 1. The toxicity bioassay (Microtox test) showed that elimination of BPA by both strains is accompanied by reduction of its toxic effect. The ability of tested strains to degrade BPA combined with their high resistance to this xenobiotic indicates that Acinetobacter sp. K1MN and Pseudomonas sp. BG12 are potential tools for BPA removal during wastewater treatment plant.
“…3,5,9,12–15 Therefore, providing an efficient eco-friendly solution for its removal from exposed natural environments is so necessary. According to the results of several studies 3,5,6,8,9,11,16–24 as well as our previous research, 25 bioremediation using BPA-degrading bacteria has been proved to be an effective way to remove this compound. Up to now, many BPA-degrading bacteria have been isolated from soil, sediment, water and petrochemical wastewater and BPA-biodegradation pathways have been recognized in most of them.…”
Section: Introductionmentioning
confidence: 89%
“…Up to now, many BPA-degrading bacteria have been isolated from soil, sediment, water and petrochemical wastewater and BPA-biodegradation pathways have been recognized in most of them. [3][4][5][6][7][8][9]11,[16][17][18][19][20][21][22][23][24][25][26][27][28][29] Lobos et al 19 and Spivack et al 30 were the rst to describe two major and minor pathways for BPAbiodegradation by Sphingomonas sp. strain MV1.…”
The efficient biodegradation of bisphenol A (BPA) and phenol in salty petrochemical wastewater using a novel indigenous halotolerant, Pseudomonas sp. The bacterium has potential to be used for petrochemical and similar wastewaters treatment.
“…Bacterial species belonging to Stenotrophomonas were previously found to be capable of using either nonylphenol or octylphenol as a sole carbon source. 44 For species belonging to the well-known human-pathogenic and plant association Enterobacter, degradation of EDCs has been reported particularly for bisphenol A 45 , polychlorinated biphenyls 46 , endosulfan 47 , dibutyl phthalate 48 and nonylphenol 49 .…”
Section: Degradation Of Nonylphenol By Bacterial Speciesmentioning
Endocrine disrupting chemicals (EDCs) are synthetic chemicals that alter the function of endocrine systems in animals including humans. EDCs are considered priority pollutants and worldwide research is ongoing to develop bioremediation strategies to remove EDCs from the environment. An understanding of indigenous microorganisms is important to design efficient bioremediation strategies. However, much of the information available on EDCs has been generated from developed regions. Recent studies have revealed the presence of different EDCs in South African natural resources, but, to date, studies analysing the capabilities of microorganisms to utilise/degrade EDCs have not been reported from South Africa. Here, we report for the first time on the isolation and enrichment of six bacterial strains from six different soil samples collected from the Mpumalanga Province, which are capable of utilising EDC nonylphenol as a carbon source. Furthermore, we performed a preliminary characterisation of isolates concerning their phylogenetic identification and capabilities to degrade nonylphenol. Phylogenetic analysis using 16S rRNA gene sequencing revealed that four isolates belonged to Pseudomonas and the remaining two belonged to Enterobacteria and Stenotrophomonas. All six bacterial species showed degradation of nonylphenol in broth cultures, as HPLC analysis revealed 41–46% degradation of nonylphenol 12 h after addition. The results of this study represent the beginning of identification of microorganisms capable of degrading nonylphenol, and pave the way for further exploration of EDC-degrading microorganisms from South Africa.
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