A number of methods for in vitro culture of periodontal ligament fibroblasts were investigated. One successful method is described. Such cells can be maintained readily in subculture. Indications of growth of these cells on sections of enzymatically debrided teeth are reported.

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Rhesus monkey periodontal ligament-derived fibroblasts were cultured on glass, Vitallium, poly(methyl methacrylate) and enzymatically debrided teeth. Scanning electron micrographs of these preparations and of the periodontal ligament surrounding normal and replanted teeth were compared. The fibroblasts cultured in vitro could organize on implant material and enzymatically debrided teeth to produce a network with fibers resembling those that are seen in the normal periodontal ligament.