Meiotic and pollen viability studies were conducted in Passiflora edmundoi Sacco (n = x = 9). For meiotic studies anthers were squashed in a drop of 1% acetic carmine. For analysis of pollen viability, pollen grains of flowers at anthesis were stained with two stains, lugol and Alexander stain. At diakinesis frequency of bivalents was high (86% of pollen mother cell observed) and it was observed other pairing patterns (7 II + 1 IV; 5 II + 2 IV; and 18 I). The average number of chiasmata per cell was 9.3, in concordance with high frequency of bivalents. Meiotic phases showed the presence of spindle irregularities, asynchrony, and other abnormalities as laggard chromosomes and anaphase bridges. Different post-meiotic products were observed and the frequency of triads was higher than monads, dyads, and polyads; however the meiotic index was 97.2% and the pollen viability was 97.9% suggesting meiotic stability. Sterile pollen was sorted in two categories: empty (60.3%) and shrunken (contracted cytoplasm) grains. The high meiotic index, pollen viability, frequency of chiasmata and bivalent pairing indicate that the species is meiotic stable and can be used in selection programs and intra and inter-specific crosses beyond the meiotic irregularities observed.
Summary It was described karyotypes of 6 species (2nϭ18) collected in Rio de Janeiro, P. alata, P. edmundoi, P. malacophylla, P. mucronata, P. galbana, P. quadrangularis. To do that young root tips were either obtained from seedling or adult plants. The arm ratio for each chromosome (r), total haploid chromatin length (THC), and asymmetry index (TF%) were calculated. Some differences as THC, TF%, position of the centromere, and number, type and position of satellites were observed among the species studied. The species showed metacentric and submetacentric chromosomes, except P. galbana that showed only metacentric chromosomes. P. malacophylla revealed the smallest THC (14.67 µm) while P. quadrangularis revealed the largest (33.58 µm). The karyotypes varied in relation to other specimens studied and this result, allied to chromosome segregation studies, suggests the existence of intraspecific karyotypic variation in Passiflora species.
A Lesão por pressão (LPP) é o resultado da perda de integridade da pele e tecidos subjacentes que ocorre quando há aumento da pressão sob a pele, também está relacionada ao com a fricção e cisalhamento. Desse modo, as ações de prevenção dos profissionais de enfermagem visam impedir o surgimento e progressão deste agravo. Sendo assim, este estudo trata-se de uma revisão bibliográfica do tipo revisão integrativa da literatura, que buscou artigos nas bases de dados Biblioteca Científica Eletrônica Online (SCIELO), Literatura Latino Americana e do Caribe em Ciências da Saúde (LILACS) e Bases de Dados de Enfermagem (BDENF) e elaborou uma síntese acerca dos resultados encontrados. Como as principais contribuições de enfermagem para promoção da prevenção da LPP, está a aplicação da escala de Braden, mudança de decúbito, diminuição da umidade da pele, aplicação de coberturas de fibra e hidrocoloide, educação em saúde para os pacientes e atualização profissional paar os enfermeiros.
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