Meiotic irregularities and pollen viability in<i>Passiflora edmundoi</i>Sacco (Passifloraceae)

Souza, Margarete Magalhães; Pereira, Telma Nair Santana; Viana, Alexandre Pío; Pereira, Messias Gonzaga; Bernacci, Luís Carlos; Sudré, Cláudia Pombo; Silva, Leandro da Cruz

doi:10.1080/00087114.2003.1010589320

Cited by 40 publications

(32 citation statements)

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“…But, though only bivalents are expected, different configurations of chromosome pairing were observed in Passiflora species in this work, as observed for other species of this genus. In P. edmundoi configurations as 7II+IV and 5II+2IV were observed (Souza et al 2003a). High degree of chromosomal pairing and formation of chiasmata indicate homology of chromosomes or of chromosomal regions (Nikolova & Niemirowicz-Szczytt 1995).…”

Section: Discussionmentioning

confidence: 99%

Meiotic behavior in wild and domesticated species of Passiflora

Souza¹,

Pereira²

2011

Rev. bras. Bot.

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-(Meiotic behavior in wild and domesticated species of Passiflora). The meiotic behavior of fourteen Passiflora taxa was analyzed. The species were grouped according to the n value (6, 9 and 12) for statistical studies. Some species presented tetravalent associations or univalent chromosomes in diakinesis, bivalent formation prevailing. The qui-square test revealed significant differences in the chiasma frequency among species for n = 9 and n = 6 groups. There was predominance of interstitial chiasmata in almost all studied species. The n = 12 group was the only one whose meiotic behavior was considered similar due to the quantity of chiasmata per cell, tendency of interstitial chiasma localization. Some species presented meiotic irregularities, such as laggard and precocious chromosomes in meiosis I. In telophase II the percentages of meiotic irregularities was low. Irregularities in the spindle orientation were presented in higher percentages in the end of meiosis II, and were also responsible for post-meiotic abnormal products. The irregularities observed during meiosis can have influence on the percentage of sterile pollen grains and success of interspecific crossings in Passiflora species.Key words -chiasmata, meiotic index, microsporogenesis, passion flower, post-meiotic products RESUMO -(Comportamento meiótico em espécies silvestres e domesticadas de Passiflora). O comportamento meiótico de 14 táxons foi analisado. As espécies foram agrupadas de acordo com o valor de n (6, 9 e 12) para estudos estatísticos. Algumas espécies apresentaram associações tetravalentes ou cromossomos univalentes em diacinese, prevalecendo formação de bivalentes. O teste de qui-quadrado revelou diferenças significativas na freqüência de quiasmas entre espécies para grupos com n = 9 e n = 6. Houve predominância de quiasmas intersticiais em quase todas as espécies estudadas. O grupo com n = 12 foi o único cujo comportamento meiótico foi considerado similar devido à quantidade de quiasmas por célula, tendência à localização intersticial de quiasmas. Algumas espécies apresentaram irregularidades meióticas, tais como cromossomos retardatários ou precoces em meiose I. Na telófase II os percentuais de irregularidades meióticas foram baixos. Irregularidades na orientação das fibras dos fusos ocorreram em alto percentual no final da meiose II, sendo também responsáveis por produtos pós-meióticos anormais. As irregularidades observadas durante a meiose podem ter influência sobre o percentual de grãos de pólen estéreis e o sucesso de cruzamentos interespecíficos em espécies de Passiflora.Palavras-chave -índice meiótico, microsporogênese, produtos pós-meióticos, quiasmas

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Section: Discussionmentioning

confidence: 99%

Meiotic behavior in wild and domesticated species of Passiflora

Souza¹,

Pereira²

2011

Rev. bras. Bot.

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“…Five plants were analyzed (repetitions) per accession, and at least 500 PG were counted per repetition/accession. The PG were classified into two types (Souza et al 2003): type 1 (T1), empty (absence of cytoplasm), and type 2 (T2), contracted cytoplasm. The lengths of 50 viable and unviable PG per accession were measured using a micrometric ocular.…”

Section: Pollen Viabilitymentioning

confidence: 99%

Meiotic irregularities in Capsicum L. species

Souza

Pereira

Sudré

et al. 2012

Crop Breed. Appl. Biotechnol.

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-Cytogenetic and pollen viability (PV) studies were performed in pepper accessions, Capsicum chinense and Capsicum baccatum. Irregularities such as laggard and univalent chromosomes, bridges, problems in the spindle fibers and cytomixis were observed, especially in C. baccatum which was the most unstable genotype. In the post-meiotic products, irregularities were observed, on average, at 20 % of the microspores in C. baccatum and 17 % in C. chinense. PV in C. baccatum was below 70 %, while in C. chinense, it was above 80 %. Meiotic irregularities in Capsicum, mainly in C. baccatum, considering the low PV estimated, were significant but not impeditive for fertilization.

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“…Pollenkitt protects and minimizes the dehydration of pollen grains and consequently limits the loss of viability in this species; additionally, this compound is involved in promoting the adhesion of grains to the stigma, inducing the volatilization of compounds and attracting pollinators due to its color (Souza, Pereira, Viana, Silva, & Sudre , 2004).…”

Section: Resultsmentioning

confidence: 99%

<b>Morphology and viability of castor bean genotypes pollen grains

et al. 2016

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ABSTRACT. The objective of this work was to characterize the morphology and viability of the pollen of 15 genotypes of castor bean (Ricinus communis L.) and to generate information that can assist in the selection of highly promising male parents for future use in genetic improvement programs aimed at producing seeds for oil extraction. Acetolysis and scanning electron microscopy was used to characterize the morphology of the pollen. The viability of the pollen grains was estimated by in vitro germination and colorimetric analysis (acetocarmine 2% and 2, 3, 5-triphenyltetrazolium chloride 1%). For the in vitro germination, pollen grains were grown in 10 types of solidified culture medium consisting of different concentrations of sucrose, boric acid, calcium nitrate, magnesium sulfate and potassium nitrate. The pollen grains had the following characteristics: medium size, isopolar and subspheroidal shape, radial symmetry, circular ambit, 3-colporate, elongated endoapertures, tectate exine and granulated sexine. The acetocarmine dye overestimated pollen viability. The media M5 and M8 were the most efficient at promoting the germination of pollen grains. The studied genotypes had high levels of viability and can therefore be used as male parents in genetic improvement programs.Keywords: Ricinus communis L., in vitro germination, colorimetric analysis, pollen tube.Morfologia e viabilidade de grãos de pólen de genótipos de mamoneira RESUMO. O objetivo deste trabalho foi caracterizar a morfologia e a viabilidade polínica em quinze genótipos de mamoneira (Ricinus communis L.), a fim de gerar informações que possam auxiliar a seleção de genitores masculinos altamente promissores para posterior utilização em programas de melhoramento, tendo em vista a produção de sementes para a extração de óleos. As descrições morfopolínicas foram feitas a partir da acetólise e microscopia eletrônica de varredura. A viabilidade dos grãos de pólen foi estimada por meio da germinação in vitro e análise colorimétrica (carmim acético 2% e 2, 3, 5-cloreto de trifeniltetrazólio 1%). Para a germinação in vitro, grãos de pólen foram inoculados em dez tipos de meio de cultura, constituídos de diferentes concentrações de sacarose, ácido bórico, nitrato de cálcio, sulfato de magnésio e nitrato de potássio e solidificado. Os genótipos apresentam grão de pólen de tamanho médio, isopolares, subesferoidal, simetria radial, âmbito circular, 3-colporados, endoaberturas lalongadas, exina tectada e sexina granulada. O corante carmim acético superestima a viabilidade de pólen. Os meios M5 e M8 foram os mais eficientes na germinação dos grãos de pólen. Os genótipos estudados podem ser utilizados como parental masculino em programas de melhoramento, já que apresentam altos índices de viabilidade. Palavras-chave:Ricinus communis L., germinação in vitro, análise colorimétrica, tubo polínico.

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Meiotic irregularities and pollen viability inPassiflora edmundoiSacco (Passifloraceae)

Cited by 40 publications

References 32 publications

Meiotic behavior in wild and domesticated species of Passiflora

Meiotic behavior in wild and domesticated species of Passiflora

Meiotic irregularities in Capsicum L. species

<b>Morphology and viability of castor bean genotypes pollen grains

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