No abstract

No abstract

No abstract

No abstract

Nanoencapsulation of active compounds using poly-(d,l-lactide-co-glycolide) (PLGA) is commonly used in the pharmaceutical industry for drug delivery and may have important applications in the food industry. Control of growth of foodborne bacteria with the goals of reducing the number of foodborne illness outbreaks, assuring consumers a safer food supply remains a priority in the food industry. Natural antimicrobials are an excellent way to eliminate pathogens without introducing chemical preservatives that consumers may find undesirable. Cinnamon bark extract (CBE) is an effective pathogen inhibitor isolated from cinnamon spice. PLGA nanoparticles containing CBE were produced using an emulsion-solvent evaporation method and characterized for size, polydispersity, morphology, entrapment efficiency, in vitro release and pathogen inhibition. PLGA with 2 different ratios of lactide to glycolide (65:35 and 50:50) were used to determine how polymer composition affected nanoparticle characteristics and antimicrobial potency. The size of the nanoparticles ranged from 144.77 to 166.65 nm and the entrapment efficiencies of CBE in 65:35 PLGA and 50:50 PLGA were 38.90% and 47.60%, respectively. The in vitro release profile at 35 °C showed an initial burst effect for both types of PLGA followed by a more gradual release of CBE from the polymer matrix. Both types of PLGA nanoparticles loaded with CBE were effective inhibitors of Salmonella enterica serovar Typhimurium and Listeria monocytogenes after 24 and 72 h at concentrations ranging from 224.42 to 549.23 μg/mL. The PLGA encapsulation improved delivery of hydrophobic antimicrobial to the pathogens in aqueous media.

Guabiroba fruit has been highlighted for its high phytochemical content, particularly of phenolic compounds. The stability, bioavailability, and bioactivity of these compounds can be enhanced by nanoencapsulation, to improve functionality. Poly(d,l-lactic-co-glycolic) acid (PLGA) nanoparticles containing phenolic extract of guabiroba (GPE) were synthesized by an adapted emulsion-evaporation method and their physico-chemical and functional properties were studied at two lactic to glycolic acid ratios (50:50 and 65:35). Higher (P<0.05) or equivalent antioxidant capacity compared to free GPE were observed for GPE-loaded nanoparticles. Free extract and PLGA nanoparticles were effective inhibitors of Listeria innocua, with lower (P<0.05) GPE concentrations required for inhibition when nanoencapsulated. Also, reduction of ROS generation in non-cancer cells was achieved with lower GPE concentrations (P<0.05) after encapsulation. These results suggest that PLGA nanoparticles can be used as a delivery system for phenolic compounds at lower levels than originally required for enhanced functional properties.

No abstract

No abstract