Significant differences were found in the levels of urokinase (uPA), tissue plasminogen activator (tPA), and PAI-1 inhibitor between glioblastomas and breast cancer metastases and the histologically unaltered (relatively intact) tissue around meningioma lesions (p≤0.05 in all cases). The levels of uPA-AG and uPA-act in meningioma were higher than those in the relatively intact tissue, while the levels of both tPA forms were reduced. The levels of uPA-AG and uPA-act in both malignant tumors and their perifocal areas were elevated compared to those in the relatively intact tissue. The levels of both tPA forms were reduced in all other tissues, except for glioblastoma. The level of PAI-1 inhibitor in malignant tissues was higher (being predominant in tumors) compared to that in the intact tissue surrounding meningioma, as well as relative to that in meningioma. The study proves that uPA and its inhibitor PAI-1 are directly involved in the metabolism of malignant gliomas and brain metastases of breast cancer. The role of tPA is to protect meningiomas; tPA activation in malignant brain tumors is suppressed.
84 Background: Esophageal squamous cell carcinoma (ESCC) is 2 to 8 times more frequent in men than in women. Tumor progression is directly connected with plasmin participation in destruction of the extracellular matrix and the basement membrane surrounding tumor. The purpose of the study was a comparative analysis of plasminogen (PG), free plasmin (P) and plasmin bound in complex with α2-antiplasmin (PAP) in ESCC tissues of men (M) and women (W). Methods: ESCC tumor tissue (T) and its perifocal zone (PZ) were studied by the ELISA (19 men and 8 menopausal women aged 58-72, st II, G2, T2-3N0-1M0-1). The results were compared to the histologically unchanged tissue from the resection line (RL). PG, P and PAP were measured in 10% homogenates prepared in sterile saline, with recalculation per 1 g wet tissue). Data were processed using Statistica 10. Results: PG content in RL was 1.5 times higher in M than in W. PG in T of M was decreased by 2 times, in W by 3.3 times, in PZ of M – by 2.9 times, in W – by 2.3 times. PG in T and PZ of M was 2.5 and 1.2 times higher than in W. P content in RL of all patients was significantly lower than in other samples, but 1.4 times higher in M than in W. P in T and PZ of M was 3 and 2.5 times higher than in RL, in T and PZ of W – 3.5 and 2.8 times higher than in RL. Significant differences were observed between P levels in T (but not in PZ): 1.3 times higher in M than in W. PAP content was relatively constant in all tissues, with a slight prevalence in T and PZ of W. P/PAP ratio was elevated in all tissues compared to RL, but in T and PZ of M it was 1.4 and 1.2 times higher than in W. P/PAP in RL of M was 1.4 times higher than in W. Conclusions: The results show a higher aggressiveness of P in PZ and T of M, while P formation in T of W was less active. We reported on a higher content of PG activators in T and PZ of M, and taking into account the morbidity statistics and literature data we suppose the effect of androgens on the conditions of PG activation into P and manifestation of its biological features. P in PZ of both M and W can contribute in the expansion of a “tumor field”. P binding in PAP complex was similar in all studied tissues.
258 Background: Our purpose was to study the protease/protease inhibitor (P/PI) balance in the blood plasma of patients with cancer of the head of the pancreas before and after pancreatoduodenal resection (PDR) with postoperative complications. Methods: The study was performed using clinical observation, biochemical examinations and statistical analysis in Microsoft Office Excel 2010. The blood plasma of 92 patients with pancreatic head cancer (53 men and 39 women aged 45-76 years, Т2-4N0M0) was studied before the surgery (b/s) and on days 1, 7, 14 and 17 after PDR. The patients were divided into two groups: g1 – 69 patients without postoperative (p/o) complications and g2 – 23 patients with p/o complications: generalization – 7, thrombosis - 8, acute postoperative pancreatitis - 2, gastrostasis - 2, anastomotic leakage - 4 patients. Kinetics of trypsin-like proteases (TLP) and α-1-proteinase inhibitor (α1PI) was studied by spectrophotometry. The data were compared with the blood plasma of 39 healthy donors (N). Results: TLP activity b/s exceeded N in g1 and g2 by 4.1 and 10.6 times; TLP in g2 was 2.6 times higher than in g1. The α1PI activity b/s was higher than N by 1.2 times (p < 0.05) in g1 and lower than N by 2.0 times in g2; α1PI in g2 was 2.4 times lower than in g1. After PDR, activity of TLP increased in all patients on day 1 but decreased on days 7-14 in g1 remaining 2.7 times higher than N by the discharge. The TLP activity in g2 by the discharge was similar to levels b/s and exceeded g1 by 4.4 and N by 12.1 times. The α1PI activity after PDR increased in all patients on days 1-17, but in g1 by the discharge it was similar to N and in g2 it was 1.5 times lower than N. The TLP/α1PI ratio was higher in g2 than in g1 at all times. Conclusions: A high TLP activity and a low α1PI activity, compared to N, were maintained in the blood plasma of all patients with p/o complications, despite their types. The P/PI balance in g2 was shifted to the left being 5.5-9.0 times higher than in g1 at all times which allowed the prognosis of postoperative complications before the surgery, perioperatively or on day 1 after the surgery.
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