Background: Capnophyllum peregrinum (L.) Lange (Apiaceae) is the unique taxon of capnophyllum genus in Algerian flora. It has never been investigated in regards to its total phenolic and flavonoid contents and antioxidant and photoprotective activities. Methods: C. peregrinum aerial parts extracted with absolute methanol. The total flavonoid and phenolic contents of the extract were evaluated to determine their correlation with the antioxidant and photoprotective activities of the extract. Results: The methanolic extract demonstrated a significant amount of phenolics and flavonoids (74.06 ± 1.23 mg GAE/g, 44.09 ± 2.13 mg QE/g, respectively) and exhibited good antioxidant activity in different systems, especially in 1,1-Diphenyl-2-picrylhydrazyl (DPPH), reducing power and total antioxidant capacity assays. Furthermore the extract showed high photoprotective activity with the sun protection factor (SPF) value = 35.21 ± 0.18. Conclusions: The results of the present study show, that the methanolic extract could be used as a natural sunscreen in pharmaceutics or cosmetic formulations and as a valuable source of natural antioxidants.
Water-distilled essential oil of the aerial parts of Daucus reboudii Coss. was analysed by GC-MS and GC-FID. Twenty-four compounds were identified, corresponding to 87.2% of the oil, mainly represented by monoterpenes. The major constituents were α α α α α-pinene (39.7%) and sabinene (21.2% Gas ChromatographyGC analysis were performed with a Varian 3400 gas chromatograph equipped with a FID and with a fused silica capillary column from Macherey Nagel (25 m × 0.25 mm i.d., 0.25 µm film thickness) coated with an apolar phase Optima 5 (5% phenyl, 95% dimethylpolysiloxane). The chromatogram was realized by holding the oven temperature at 60°C, then the temperature was programmed from 60°C to 280°C at a rate of 3°C/min. Injector and detector were maintained at a temperature of 280°C. Nitrogen was used as the carrier gas. Quantization and retention times were carried out using Star 4.51 software. Gas Chromatography-Mass SpectrometryThe sample was analysed by GC-MS. The analysis were performed on an Agilent 6890 gas chromatograph equipped with a
The aim of the present work was to evaluate the antioxidant activity of extracts and four flavonoids that had been isolated from the aerial parts of Bunium alpinum Waldst. et Kit. (Apiaceae) and Tamarix gallica L. (Tamaricaceae). In this work, the four flavonoids were first extracted via various solvents, then purified through column chromatography (CC) and thin layer chromatography (TLC). The four compounds were subsequently identified by spectroscopic methods, including: UV, mass spectrum 1H NMR and 13C NMR. The EtOAc extract of Bunium alpinum Waldst. et Kit yielded quercetin-3-O-β-glucoside (3’,4’,5,7-Tetrahydroxyflavone-3-β-D-glucopyranoside) (1), while the EtOAc and n-BuOH extracts of Tamarix gallica L. afforded 3,5,3’-trihydroxy-7,4’-dimethoxyflavone (2), 3,5,7-trihydroxy-4’-methoxyflavone (3) and 5-hydroxy-3,7,4’-trimethoxyflavone (4). The antioxidant activity of the extracts and the flavonoids were then evaluated through DPPH free radical-scavenging assay. Of all studied extracts, the n-Butanol extract of Bunium alpinum (EC50 = 1.84 μg/ml) showed the best antioxidant activity against (DPPH). In contrast, the isolates demonstrated varying degrees of antioxidant activity: compound (1) was the more active (EC50 = 0.28 μg/ml), followed by compound (3) and (2) (EC50 = 0.309μg/ml, EC50 = 0.406 μg/ml, respectively), compound (4) showed the lowest activity. All the isolated flavonoids exhibited antioxidant activity, but this was lower than the control (Trolox). In conclusion, due to the presence of flavonoids in their ariel parts, the studied plants could be natural sources of several important antioxidant agents
The essential oils of the aerial parts of Kundmannia sicula (L.) DC collected from two Algerian localities, El Kala (near the coast) and Béjaia (from a meadow about 10 km from the coast), were analysed by GC and GC-MS. Although both samples showed similar overall chemical compositions, the major components of the Béjaia sample were identified as being spathulenol (14.8%), caryophyllene oxide (12.2%), salvial-4(14)en-1-one (10.1%), 1,5-epoxysalvial-4(14)ene (5.2%) and germacrene D (3.2%), while in the El Kala sample the main compounds were found to be salvial-4(14)en-1-one (16.4%), 1,5-epoxysalvial-4(14)ene (6.5%), chrysanthenyl acetate (5.2%) and α-amorphene (2.9%).
Retama sphaerocarpa Boissier is a species of the Fabaceae family [1]. This plant is used to cure rabies in the medicinal folk traditions of the east of Algeria. In the present paper we report the result obtained on the qualitative and quantitative analysis of secondary metabolites extracted from the medicinal plant R. sphaerocarpa, which has been the subject of several chemical and pharmacological investigations [2][3][4][5][6]. The aerial parts of this plant were collected during flowering in the Souk Naamane Region east of Algeria in May, June 2002.The air-dried powdered aerial parts (950 g) of R. sphaerocarpa were extracted with 70% MeOH. The MeOH extract was evaporated to dryness. The residue was dissolved in boiling water and extracted with ethyl acetate and n-BuOH successively. The solvents was evaporated and the residue of the ethyl acetate and n-BuOH extracts was dissolved in small volumes of MeOH. Two-dimensional paper chromatography using 15% AcOH and BAW (n-BuOH-AcOH-H 2 O, 4:1:5 upper phase) as solvents shows that the acetate and n-BuOH extract contains almost the same compounds representing flavonoids. The n-BuOH extract was applied to a column of polyamide MN SC6 and eluted with a gradient of toluene-MeOH with increasing polarity. Three flavonoids (1-3) contained in several fractions were isolated by preparative PC on Whatman 3MM paper using 15% AcOH, then by preparative TLC on polyamid DC6 eluted with H 2 O-MeOH-methylethylketone-acetylacetone, 13:3:3:1.The isoflavonoid glycoside (genistein 7-O-β-glucoside) was isolated from the intermediate fraction by crystallization. This compound has already been isolated from this plant [2]. Purification of each compound for spectral analysis was carried out using MeOH over Sephadex LH-20. These compounds were identified using UV and 1 H NMR spectra, chemical transformations, and comparison with authentic samples [7][8][9]. Flavonoids 1-2 are isolated for the first time from this genus. Compound 3 has been reported previously from another species of Retama [10] and from R. sphaerocarpa for the first time.
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