In Vitro Assessment of Total Phenolic and Flavonoid Contents, Antioxidant and Photoprotective Activities of Crude Methanolic Extract of Aerial Parts of Capnophyllum peregrinum (L.) Lange (Apiaceae) Growing in Algeria

Abstract

Background: Capnophyllum peregrinum (L.) Lange (Apiaceae) is the unique taxon of capnophyllum genus in Algerian flora. It has never been investigated in regards to its total phenolic and flavonoid contents and antioxidant and photoprotective activities. Methods: C. peregrinum aerial parts extracted with absolute methanol. The total flavonoid and phenolic contents of the extract were evaluated to determine their correlation with the antioxidant and photoprotective activities of the extract. Results: The methanolic extract demonstrated a significant amount of phenolics and flavonoids (74.06 ± 1.23 mg GAE/g, 44.09 ± 2.13 mg QE/g, respectively) and exhibited good antioxidant activity in different systems, especially in 1,1-Diphenyl-2-picrylhydrazyl (DPPH), reducing power and total antioxidant capacity assays. Furthermore the extract showed high photoprotective activity with the sun protection factor (SPF) value = 35.21 ± 0.18. Conclusions: The results of the present study show, that the methanolic extract could be used as a natural sunscreen in pharmaceutics or cosmetic formulations and as a valuable source of natural antioxidants.

Effect of extraction solvent on qualitative and quantitative analysis of major phyto-constituents and in-vitro antioxidant activity evaluation of Cadaba rotundifolia Forssk leaf extracts

“…Of those alternatives, the natural sources originated antioxidants have been found as the champion one. Plants are in the front line as well-known homes of various natural antioxidants; which currently have been gained serious attentions (Khatoon et al, 2013;Lefahal et al, 2018;Truong et al, 2019). These medicinal plant based natural antioxidants have played tremendous roles on fighting free radicals via the chemistry of providing an electron and/or hydrogen and making the restless oxidative species stable (Rebaya et al, 2015).…”

Can natural products improve skin photoprotection?

“…Aqueous extract 1 µL/mL 8 (Rosa et al 2008) Green propolis and red propolis Ethanolic and glycolic extract in a gel 0.2 µL/mL 2.2-5.8 (Nascimento et al 2009) Pothomorphe umbellate root Crude ethanol-water extract 0.2 µg/mL 21 (Da Silva et al 2005) Sonchus oleraceus Aqueous extract 1 µL/mL 6 (Rosa et al 2008) Brassica oleracea Aqueous extract 1 µL/mL 5 (Rosa et al 2008) Porophyllum ruderale Aqueous extract 1 µL/mL 5 (Rosa et al 2008) Plectranthus barbatus Aqueous extract 1 µL/mL 2 (Rosa et al 2008) Cyperus rotundus Aqueous extract 1 µL/mL 1 (Rosa et al 2008) Rutin -0.1% 1.1 (Velasco et al 2008) Passiflora incarnata Dry extract 1.68% 1.2 (Velasco et al 2008) Plantago lanceolata Hydroglycolic extract 2.78% 1.1 (Velasco et al 2008) Dracocephalum moldavica Ethyl acetate extract 2 mg/mL 24.79 (Khazaeli & Mehrabani 2008) Viola tricolor Ethyl acetate extract 2 mg/mL 25.69 (Khazaeli & Mehrabani 2008) Moringa oleifera Aqueous extract 4.0% 2.01 (Baldisserotto et al 2018) Capnophyllum peregrinum Crude methanolic extract 2 mg/mL 35.21 (Lefahal et al 2018) demonstrated by several previous studies (Wagner 2011). The SPF value is dependent on the molecule(s) present in the extracts that exhibit photoprotection ability, the method of extraction, the concentration of the substances, and the solvent or system in which it is incorporated.…”

Screening of in vitro sun protection factor of some medicinal plant extracts by ultraviolet spectroscopy method

“…Whereas the in vivo test is time consuming and includes various degrees of variability. The SPF numbers of extracts were calculated by applying Mansur mathematical equation in the UV-B region, which is considered to be the region of highest incidence during the day and people are exposed for a longer time [34]. Whereas plant literature shows that natural substances extracted from plants have potential sunscreen activity as it gets absorbed in the UV region due to the presence of antioxidants [35].…”