Evaluation of the antioxidant activity of extracts and flavonoids obtained from Bunium alpinum Waldst. & Kit. (Apiaceae) and Tamarix gallica L. (Tamaricaceae)

Abstract

The aim of the present work was to evaluate the antioxidant activity of extracts and four flavonoids that had been isolated from the aerial parts of Bunium alpinum Waldst. et Kit. (Apiaceae) and Tamarix gallica L. (Tamaricaceae). In this work, the four flavonoids were first extracted via various solvents, then purified through column chromatography (CC) and thin layer chromatography (TLC). The four compounds were subsequently identified by spectroscopic methods, including: UV, mass spectrum 1H NMR and 13C NMR. The EtOAc extract of Bunium alpinum Waldst. et Kit yielded quercetin-3-O-β-glucoside (3’,4’,5,7-Tetrahydroxyflavone-3-β-D-glucopyranoside) (1), while the EtOAc and n-BuOH extracts of Tamarix gallica L. afforded 3,5,3’-trihydroxy-7,4’-dimethoxyflavone (2), 3,5,7-trihydroxy-4’-methoxyflavone (3) and 5-hydroxy-3,7,4’-trimethoxyflavone (4). The antioxidant activity of the extracts and the flavonoids were then evaluated through DPPH free radical-scavenging assay. Of all studied extracts, the n-Butanol extract of Bunium alpinum (EC50 = 1.84 μg/ml) showed the best antioxidant activity against (DPPH). In contrast, the isolates demonstrated varying degrees of antioxidant activity: compound (1) was the more active (EC50 = 0.28 μg/ml), followed by compound (3) and (2) (EC50 = 0.309μg/ml, EC50 = 0.406 μg/ml, respectively), compound (4) showed the lowest activity. All the isolated flavonoids exhibited antioxidant activity, but this was lower than the control (Trolox). In conclusion, due to the presence of flavonoids in their ariel parts, the studied plants could be natural sources of several important antioxidant agents

Halophytic herbs of the Mediterranean basin: An alternative approach to health

“…Stem bark contains coniferyl alcohol 4-sulphate and other phenolic and flavonoid sulphates, such as kaempferol 4′methyl ether 3-sulphate, and tamarixetin 3-sulphate were also identified (Tomas- Barberán et al, 1990). Other reported flavonoids include 3,5,3′-trihydroxy-7,4′-dimethoxyflavone, 3,5,7-trihydroxy-4′-methoxyflavone and 5-hydroxy-3,7,4′-trimethoxyflavon, O-Methylated and glucuronosylated flavonoids, as well as rhamnetin, tamarixetin, rhamnazin, KGlcA, KGlcA-Me, QGlcA, and QGlcA-Me (Hmidene et al, 2017;Lefahal et al, 2017). Also, pronounced levels of carnosol, capsaicin, 6shogaol, 6-gingerol and their corresponding derivatives, and even vitamins, terpenoids (carotenoids and essential oils) have been identified (Boulaaba et al, 2013;Ksouri et al, 2009), which makes this plant a potential source of bioactive compounds for multiple applications.…”

Phenolic Compounds From Indonesian White Turmeric (Curcuma Zedoaria) Rhizomes

“…Compound 2 had been isolated previously from Alpinia galanga (Zingiberaceae), Tecomaria capensis var. aurea (Bignoniaceae), and Tamarix gallica (Tamaricaceae) [36][37][38]. Furthermore, compound 3 had been reported previously from Matricaria chamomilla, Zanthoxylum zanthoxyloides, and Alpinia calcarata (Zingiberaceae) [39][40][41].…”

A Systematic Review on Phytochemistry, Ethnobotany and Biological Activities of the Genus Bunium L.

“…[71] Several classes of natural compounds have been reported mainly comprising fatty acids, terpenoids, saponins, polyacetylenes, coumarins, anthraquinones, tannins, flavonoids, organic acids, saccharides and proteins (Table 3). For B. alpinum, [68] B. fontanesii [18]…”