The variant genotype thiopurine S-methyltransferase has been associated with the occurrence of leucopenia. The involvement of polymorphisms in inosine triphosphate diphosphatase and xanthine dehydrogenase genes in the development of digestive intolerance and pancreatitis will require further verification.

No abstract

Azathioprine-induced acute pancreatitis should not be considered as an absolute contraindication for the use of MP. Further investigation is required to create a better understanding of the mechanism underlying the adverse events and to allow more possibilities for personalised therapy.

No abstract

The purpose of the study was to monitor the antioxidative effect of morine in alloxan-induced diabetes mellitus in laboratory rat. The animals were divided by random selection into two groups (n = 7). The treated group was given morine in oral doses of 10 mg/kg -1 in 0.5% solution of Methocel E5 once a day; the control diabetic group was given only the solution of Methocel E5. Once a week, selected laboratory indices were determined in all animals (glucose, urea and cholesterol levels in serum, total glucose and protein losses through urine); diuresis as well as antioxidative enzymes (superoxiddismutase, glutathione peroxidase); total antioxidative capacity and malondialdehyde level in the blood. On the 20 th day the animals were exsanguinated and kidney tissue and pancreas samples were taken for histopathological analysis.We found a significant increase (p ≤ 0.05) of the glutathione peroxidase catalytic activity in the treated group compared to control diabetic group. There was also a highly significant increase (p ≤ 0.01) of total antioxidative capacity in the treated group compared to control diabetic group. A significant decrease (p ≤ 0.05) of malondialdehyde level was identified in the treated group compared to the control diabetic group. The superoxiddismutase catalytic activity involved nonsignificant changes. A significant decrease (p ≤ 0.05) of cholesterol level in serum was identified in the treated group compared to control diabetic group. Other examined laboratory parameters did not exhibit significant changes.Biochemical indices followed in this study indicated a protective antioxidative effect of morine. However, the results of histopathological examination did not correlate with them.

No abstract

The aim of this study was in vivo testing of the action of three newly synthesized potential ultrashort acting beta-blockers on the heart rate in the laboratory rat. The tested substance 44Bu was administered to animals with induced tachycardia, in the form of an intravenous bolus in general anaesthesia.Doses at concentrations of 1.5 mg⋅kg -1 , 2.5 mg⋅kg -1 , and 3.5 mg⋅kg -1 of body mass were tested and the efficacy was compared with placebo. For the heart rate monitoring a computer electrocardiograph was used. Significant (p < 0.05) heart rate decrease was recorded for all three tested doses, minimally up to the 14 th minute following the intravenous administration.Bradycardic effect of the compound 44Bu was compared with the action of esmolol under the same experimental conditions. The effects of the compound 44Bu and esmolol were not different in the onset, but in the depth of the heart rate decrease, above all at higher concentrations. It was experimentally verified, that the compound 44Bu has the properties of an ultrashort acting beta adrenergic receptor blocker.Pharmacology, ester-functional group, bradycardic effect, heart rate, rats

Various hemostatics are used for renal surgical procedures. We investigated the hemostatic efficacy of cellulose derivatives on the model of partial nephrectomy in rats focusing on the local reaction of renal parenchyma. A total of 50 Wistar rats were divided into five groups of 10 animals each. Partial nephrectomy of the caudal pole without hilar vascular control was performed. Oxidized cellulose (OC), sodium salt of oxycellulose (OCN), carboxymethyl cellulose (CMC), dialdehyde cellulose (DAC), and gelatin-based hemostatic (C) were applied to the bleeding wounds. The time to hemostasis was monitored. Half of the animals were euthanized after 3 days, the second half 30 days from the experiment start date. The left kidney was excised and subjected to histopathological examination. The biochemical data was subjected to statistical analysis. The time to hemostasis in all groups was significantly less than in the C group (in OC p = 0.0057, OCN p = 0.0039, CMC and DAC p = 0.0001). In the C group, massive hemorrhages and necrosis did occur. In the OC and OCN groups, there were regenerative changes, a receding inflammatory reaction and hemorrhage. DAC caused an immune reaction and massive interstitial hemorrhages with biochemical signs of liver damage. Parenchyma in CMC revealed a reduction of necrosis and interstitial hemorrhages with regenerative processes. The most effective hemostatics were CMC and OC, achieving the best results both in the time to hemostasis, and for histopathological evaluation.