Monitoring of Antioxidative Effect of Morine in Alloxan-induced Diabetes mellitus in the Laboratory Rat

Bartošíková, Lenka; Nečas, Jiří; Suchý, Václav; Kubínová, Renata; Vesela, Daniela; Beneš, L; Bartošík, Tomáš; Illek, J.; Šalplachta, J.; Klusáková, Jarmila; Bartošová, Ladislava; Strnadová, Veronika; Fráňa, Petr; Fráňová, Jana

doi:10.2754/avb200372020191

Cited by 22 publications

(14 citation statements)

References 23 publications

(17 reference statements)

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“…20) Thus alloxan induced diabetes mellitus served as a pathological biomodel for testing a substance with supposed antioxidant activity in vivo. 21) One of the targets of the reactive oxygen species is DNA of pancreatic islets. Its fragmentation takes place in β cells exposed to alloxan.…”

Section: Resultsmentioning

confidence: 99%

Hypoglycemic and Antilipidperoxidative Effects of a Polyherbal Formulation, Diakyur, in Experimental Animal Models

Joshi¹,

Ekambaram²,

Venkataraman

2007

JOURNAL OF HEALTH SCIENCE

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The present study investigates the effect of Diakyur, a polyherbal formulation composed of powder of Cassia javanica and dried, standardised aqueous extracts of Cassia auriculata, Salacia reticulata, Gymnema sylvestre, Mucuna pruriens, Syzygium jambolanum, Terminalia arjuna on blood glucose level of normal and diabetic animals as well as lipid peroxide level in normal and 28 day drug treated diabetic rats. The raw materials and the formulation were standardised by high performance thin layer chromatography (HPTLC) and high pressure liquid chromatography (HPLC) method of analysis. The hypoglycemic activity and glucose tolerance test were studied in normal and alloxan (150 mg/kg, i.p) induced diabetic rats and rabbits after administration of Diakyur at the dose of 1600 mg/kg, p.o. Blood glucose level was determined by O-toluidine method. Lipid peroxide levels of plasma, erythrocyte membrane, liver and kidney tissues were studied in alloxan induced diabetic rats after 28 days drug treatment. At the dose of 1600 mg/kg, p.o Diakyur showed a hypoglycemic effect at varying degree of significance (p < 0.05-0.001) in normal as well as alloxan induced diabetic rats and rabbits in comparison with respective control groups. Diakyur treatment in the glucose tolerance test showed the maximum effect at 180th min of glucose administration in both normal and alloxan diabetic animals. The drug treated alloxan diabetic rats showed significant ( p < 0.001) reduction in plasma, erythrocyte membrane, liver and kidney lipid per- * To whom correspondence should be addressed: R&D, Amrutanjan Limited, #103 Luz Church Road, Mylapore, Chennai 600 004, T. N., India. Tel.: +91-44-24994465; Fax: +91-44-24994585; E-mail: v4csj@rediffmail.com oxide levels after 28 days treatment when compared to untreated alloxan diabetic rats. The results indicate the significant hypoglycemic activity of Diakyur in both rats and rabbits, whereas an antilipidperoxidative activity in diabetic rats.

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Section: Resultsmentioning

confidence: 99%

Hypoglycemic and Antilipidperoxidative Effects of a Polyherbal Formulation, Diakyur, in Experimental Animal Models

Joshi¹,

Ekambaram²,

Venkataraman

2007

JOURNAL OF HEALTH SCIENCE

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“…A relationship between oxidative stress and diabetes mellitus has been well demonstrated in many experimental animal models Bartosikova et al, 2003).…”

Section: Discussionmentioning

confidence: 98%

Antihyperglycemic Activity ofPongamia pinnataStem Bark in Diabetic Mice

Badole

Subhash

Bodhankar

2008

Pharmaceutical Biology

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Pongamia pinnata (L.) Pierre (Fabaceae), popularly known as "Karanj" or "Karanja" in Hindi, and Indian beech in English, is a medium-sized glabrous tree. In the present study we report the antihyperglycemic activity of stem bark of alcohol extract of Pongamia pinnata (PPSBAE). Based on acute oral toxicity data, PPSBAE showed no mortality in normal mice up to 5,000 mg/kg. PPSBAE was administered as three doses (i.e., 100, 200, and 400 mg/kg) to diabetic mice, and the serum glucose level and body weight were measured. The onset of serum glucose reduction was observed at 2 h (130.32 mg/dl), peak at 4 h (151.79 mg/dl) and sustained at 6 h, but waned at 24 h. In the subacute study, maximum reduction (305.72 mg/dl) in serum glucose was observed at a dose of 400 mg/kg on day 28. An oral glucose tolerance test (OGTT) was carried out after administration PPSBAE (200 mg/kg) in non-diabetic mice previously loaded with 2.5 g/kg, per oral (p.o.) of glucose. The PPS-BAE (200 mg/kg) showed increased glucose threshold in non-diabetic mice. These results suggest that the PPSBAE possesses antihyperglycemic activity.

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“…The alloxan-induced diabetic mice had a three to four fold increase in blood relative to the normal control mice. Administration of diabetogenic drug, alloxan monohydrate, caused a selective massive destruction of the β cells of islets of Langerhans resulting in hyperglycemia [14,15]. The lowering effect of blood sugar levels by Ocimum lamiifolium in the same manner regardless of the dosage might suggest that the extract may have been absorbed in the cell system through active transport where a particular concentration saturation of the extract occurred resulting to the rest of extract being excreted [16].…”

Section: Discussionmentioning

confidence: 99%