Antibacterial efficiency of vermiculite/chlorhexidine nanocomposites and results of the in vivo test of harmlessness of vermiculite

Holešová, Sylva; Štembírek, Jan; Bartošová, Ladislava; Pražanová, Gabriela; Valášková, Marta; Samlíková, Magda; Pazdziora, Erich

doi:10.1016/j.msec.2014.05.054

Cited by 22 publications

(12 citation statements)

References 31 publications

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“…The development of suitable antibacterial materials that can be used for medical purposes is a current medical research topic. Substances that act as carriers of antibacterial or antifungal agents for topical treatment that avoid undesirable treatment of the entire body are of particular interest [12]. For practical applications, sustained antibacterial activity with regular drug release is desirable.…”

Section: Introductionmentioning

confidence: 99%

Effects of Ultrasound on Zinc Oxide/Vermiculite/Chlorhexidine Nanocomposite Preparation and Their Antibacterial Activity

et al. 2019

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Microbial infection and biofilm formation are both problems associated with medical implants and devices. In recent years, hybrid organic-inorganic nanocomposites based on clay minerals have attracted significant attention due to their application potential in the field of antimicrobial materials. Organic drug/metal oxide hybrids exhibit improved antimicrobial activity, and intercalating the above materials into the interlayer of clay endows a long-term and controlled-release behavior. Since antimicrobial activity is strongly related to the structure of the material, ultrasonic treatment appears to be a suitable method for the synthesis of these materials as it can well control particle size distribution and morphology. This study aims to prepare novel, structurally stable, and highly antimicrobial nanocomposites based on zinc oxide/vermiculite/chlorhexidine. The influence of ultrasonic treatment at different time intervals and under different intercalation conditions (ultrasonic action in a breaker or in a Roset’s vessel) on the structure, morphology, and particle size of prepared hybrid nanocomposite materials was evaluated by the following methods: scanning electron microscopy, X-ray diffraction, energy dispersive X-ray fluorescence spectroscopy, carbon phase analysis, Fourier transforms infrared spectroscopy, specific surface area measurement, particle size analysis, and Zeta potential analysis. Particle size analyses confirmed that the ultrasonic method contributes to the reduction of particle size, and to their homogenization/arrangement. Further, X-ray diffraction analysis confirmed that ultrasound intercalation in a beaker helps to more efficiently intercalate chlorhexidine dihydrochloride (CH) into the vermiculite interlayer space, while a Roset’s vessel contributed to the attachment of the CH molecules to the vermiculite surface. The antibacterial activity of hybrid nanocomposite materials was investigated on Gram negative (Escherichia coli, Pseudomonas aeruginosa) and Gram positive (Staphylococcus aureus, Enterococcus faecalis) bacterial strains by finding the minimum inhibitory concentration. All hybrid nanocomposite materials prepared by ultrasound methods showed high antimicrobial activity after 30 min, with a long-lasting effect and without being affected by the concentration of the antibacterial components zinc oxide (ZnO) and CH. The benefits of the samples prepared by ultrasonic methods are the rapid onset of an antimicrobial effect and its long-term duration.

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Section: Introductionmentioning

confidence: 99%

Effects of Ultrasound on Zinc Oxide/Vermiculite/Chlorhexidine Nanocomposite Preparation and Their Antibacterial Activity

et al. 2019

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“…This paper follows the previously published results aimed at the evaluation of toxicological and antibacterial properties of vermiculite nanocomposites [ 56 ]. The presented study describes the unique approach of using the solvent casting or impregnation methods to prepare MOFs from carmellose (a well-established mucoadhesive polymer) with the incorporation of a nanotechnologically modified clay mineral (vermiculite) and intercalated antiseptic drugs (chlorhexidine diacetate and digluconate).…”

Section: Introductionmentioning

confidence: 78%

“…Oral mucosa was simulated using a cellophane membrane glued to the surface of the slab and covered with a 5% mucin dispersion (w/w) in phosphate buffer (pH 6.8; 10 μ L/cm 2 ). The phosphate buffer (pH 6.8) [ 56 ] was maintained at 37°C and used as testing medium. The slab with attached circular MOF samples (15 mm diameter) was allowed to move up and down (samples were completely immersed in the buffer solution at the lowest point and were out of the solution at the highest point.)…”

Section: Methodsmentioning

confidence: 99%

Carmellose Mucoadhesive Oral Films Containing Vermiculite/Chlorhexidine Nanocomposites as Innovative Biomaterials for Treatment of Oral Infections

Gajdziok

Holešová

Štembírek

et al. 2015

BioMed Research International

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Infectious stomatitis represents the most common oral cavity ailments. Current therapy is insufficiently effective because of the short residence time of topical liquid or semisolid medical formulations. An innovative application form based on bioadhesive polymers featuring prolonged residence time on the oral mucosa may be a solution to this challenge. This formulation consists of a mucoadhesive oral film with incorporated nanocomposite biomaterial that is able to release the drug directly at the target area. This study describes the unique approach of preparing mucoadhesive oral films from carmellose with incorporating a nanotechnologically modified clay mineral intercalated with chlorhexidine. The multivariate data analysis was employed to evaluate the influence of the formulation and process variables on the properties of the medical preparation. This evaluation was complemented by testing the antimicrobial and antimycotic activity of prepared films with the aim of finding the most suitable composition for clinical application. Generally, the best results were obtained with sample containing 20 mg of chlorhexidine diacetate carried by vermiculite, with carmellose in the form of nonwoven textile in its structure. In addition to its promising physicomechanical, chemical, and mucoadhesive properties, the formulation inhibited the growth of Staphylococcus and Candida; the effect was prolonged for tens of hours.

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“…4). The mass loss at 30-150°C, corresponding to the removal of surface adsorbed water, dramatically decreases with the increase of surfactant loading, which suggests an increase of hydrophobicity of EVrm surface after surfactant modification (Holesova et al, 2014). The mass loss at 150-210°C, corresponding to the removal of the interlayer water associated with the interlayer cations (e.g., Mg 2+ and Ca…”

Section: Thermal Stability Of Hevrmmentioning

confidence: 97%

Structure and thermal stability of organo-vermiculite

Wei

2016

Applied Clay Science

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Antibacterial efficiency of vermiculite/chlorhexidine nanocomposites and results of the in vivo test of harmlessness of vermiculite

Cited by 22 publications

References 31 publications

Effects of Ultrasound on Zinc Oxide/Vermiculite/Chlorhexidine Nanocomposite Preparation and Their Antibacterial Activity

Effects of Ultrasound on Zinc Oxide/Vermiculite/Chlorhexidine Nanocomposite Preparation and Their Antibacterial Activity

Carmellose Mucoadhesive Oral Films Containing Vermiculite/Chlorhexidine Nanocomposites as Innovative Biomaterials for Treatment of Oral Infections

Structure and thermal stability of organo-vermiculite

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