Growth of four Trichoderma strains were tested on lignocellulosic by-products in solid state fermentation (SSF). The strains were also analyzed for their survival rate and growth after lyophilization on these carriers. All applied monocomponent and bicomponent media were substrates for the production and preservation of Trichoderma biomass. However, the maximum number of colony forming units (CFU/g dm) was acquired on bicomponent media based on dried grass and beet pulp or grass with corn cobs, when compared to monocomponent media. Although the process of lyophilization reduced the survival rate by 50%-60%, the actual number of viable cells in obtained biopreparations remained relatively high (0.58 × 108-1.68 × 108 CFU/g dm). The studied strains in the preserved biopreparations were characterized by a high growth rate, as evaluated in microcultures using the Bioscreen C system.

Anthropogenic-induced deterioration of soil health remains a global problem, resulting in a diminished productivity of agroecosystems. In order to improve soil quality, we investigated the impact of phosphorus biofertilizer on the microbiological parameters of soil (type Brunic Arenosol) degraded as a result of inappropriate cultivation and fertilization, characterized by low pH and decrease in K and Mg content.

Saskatoon berry has become as important as a commercial fruit crop. One main goal is to release new plant cultivars well adapted to different climatic and soil conditions. Dormant seeds obtained from breeding are serious problems delaying the program. The seeds were directly extracted from fruits after harvest (unstored) or after storage at −18 °C for 6 months (stored) and subjected to modified stratification (3 °C) with KNO3, H2O2, NO, smoke-water (SW) or scarification using sandpaper or H2SO4 for 10, 20, 30, 40 min or treatments with pulsed radio frequency (PRF) or red light. The seeds were also subjected to warm–cool stratification (20/3 °C). Unstored seeds germinated in a higher percentage and with better uniformity (T75–T25) than stored seeds. Stored seeds positively affected the onset of seed germination (T1) and mean germination time (MGT). Dormancy breakage was promoted by stratification with KNO3, SW or scarification with sandpaper, H2SO4 or treatments with PRF. The recommended method for the breeding program of breaking seeds dormancy is when unstored seeds are subjected to stratification in KNO3 (0.2%) or SW (1:100). Depending on the applied methods, the percentage of seeds’ germination increased to 87% compared to untreated (64%) control seeds. The positive effects of the selected methods persisted during seedling development by stimulating their growth and enhancing the chlorophyll content index (SPAD) and effective quantum yield of PSII of chlorophyll in leaves (ΦPSII).

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Ekologia względnie beztlenowych, mezofilnych, przetrwalnikujących bakterii celulolitycznych (WBMPBC) jest mało poznana. W literaturze przedmiotu znajdują się tylko nieliczne informacje dotyczące wpływu nawożenia oraz rodzaju uprawianej rośliny na występowanie w glebie WBMPBC. Dlatego celem naszych badań było wykazanie wpływu dodatku do gleby kompostów na bazie miału węgla brunatnego na właściwości chemiczne i liczebność względnie beztlenowych, mezofilnych, przetrwalnikujących bakterii celulolitycznych (WBMPBC) w glebie ryzosferowej i pozaryzosferowej wybranych roślin. Gleba pobrana z obiektów doświadczalnych była słabo kwaśna (pH – 6,2-6,4) i nieznacznie się różniła się zawartością makroelementów. Liczebność badanej grupy drobnoustrojów w glebie zależała od rodzaju kompostu i wynosiła od 9,42 do 3186,9 jtk (jednostki tworzące kolonie) w gramie suchej masy gleby (s. m. gleby). Wszystkie komposty stymulowały liczebność bakterii celulolitycznych w glebie pozarysoferowej. W przypadku ryzosfery kompost zawierający efektywne mikroorganizmy (EM) redukował liczebność WBMPBC. Największą liczebność badanej grupy drobnoustrojów stwierdzono w ryzosferze spod uprawy wiśni, nawożonej kompostem przygotowanym z węgla brunatnego z odpadem po produkcji owocników Pleuretus ostreatus (3186,9 jtk w g. s. m. gleby).

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