Heat shock protein 27 (HSP27) belongs to a family of ATP-independent chaperones and plays a fundamental role in cell physiology in various disease states, including cancer. So, it was found that serum HSP27 levels were significantly increased in patients with various tumors, but their significance in laryngeal carcinoma is not well defined. Purpose of the study. Determination and comparison HSP27 serum levels at different stages of special treatment methods in laryngeal cancer patients. Materials and methods. The studies were conducted in 31 patients of laryngeal cancer of T2–3 N0 M0 categories before treatment and at its various stages. The serum HSP27 levels analysis was carried out using the «ELISA» diagnostics test system by enzyme immunoassay. Results. The serum HSP27 levels in patients before treatment and with various types of special treatment are statistically significantly higher than the control values. There was a decrease in serum HSP27 levels in patients after removal of the neoplasm and a slight increase in serum HSP27 levels after completion of radiation therapy. Comparison of initial serum HSP27 values in patients with subsequent relapse of the disease with initial serum HSP27 levels in patients with positive treatment outcomes did not reveal statistically significant differences. Conclusion. The serum HSP27 levels in laryngeal cancer patients are elevated and remain so at all treatment stages. Initial level of serum HSP27 cannot predict tumor recurrence. Keywords: laryngeal cancer, HSP27.
Purpose of the study. To study the features of changes of spermiologic values and degree of spermatozoa DNA fragmentation depending on the presence/absence of toxocariasis invasion. Materials and methods. 89 men aged 20 to 45 years were examined, which were divided into 5 groups. The first (control) group was 12 fertile men; the second group (comparison group) – 27 infertility patients with normal level of DNA fragmentation of sperm and without antibodies to toxocariasis; The third group – 20 infertility men with normal level of DNA fragmentation of sperm and presence of antibodies to toxocariasis. By the fourth and fifth groups included 15 infertility men with high levels of DNA fragmentation of sperm and the presence of antibodies to toxocariasis and lack of respectively. A comprehensive research was conducted for all men that included analysis of spermogram according to WHO recommendations, determination of sperm DNA fragmentation and presence of toxocariasis invasion. Results. As a result of spermograms in the studied groups of men were identified the violations of ejaculate fertility (asteno-, teratoand oligozoospermia). It has been established that the degree disturbance of spermatogenesis (including significant oligo- and teratozoospermia) is depending on the fragmentation of DNA in male gametes and the presence of antibodies to toxocara. At the same time more severe patozoospermia observed in the presence of elevated levels of fragmented sperms and the absence of antibodies to toxocara antigens, which is the basis for further research. Conclusions. Thus, the determination of sperm DNA fragmentation and availability of toxocariasis invasion in men with reproductive disorders is a necessary component of a comprehensive examination, which may allow to improve the diagnostics of male infertility and to promote the development of the right tactics and optimal treatment regimens. Keywords: male infertility, spermogram, sperm DNA fragmentation, toxocariasis invasion
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