The purpose of this study was to compare weight gain and food intake during high-fat feeding in outbred CD-1 male mice while considering several different experimental designs. This study was completed using data from three separate experiments and was designed to address different experimental design issues. Experiment 1 compared mice housed in groups or singly. Experiment 2 compared adolescent and young adult mice. Experiment 3 examined mice that had been previously exercise-trained prior to diet-induced weight gain. Data from each experiment were analysed using repeated measures analysis of variance and linear regression. While housing and age did not significantly affect weight gain, mice that were previously exercise-trained consumed significantly more kilocalories than sedentary mice while maintaining comparable body weights. We generated a linear prediction model using data from Experiments 1 and 2 that will allow investigators to calculate the weeks of high-fat feeding needed to reach a target body weight. Our key findings characterize the issues related to and affecting experimental design when utilizing an outbred mouse diet-induced weight gain model and will serve as a guide for future researchers.

The recreational use of trampolines is widespread and growing rapidly around the world. The reported incidence of significant injuries continues to increase despite more than three decades of attempts to educate the public on the inherent dangers of trampolines and appropriate safety rules for their use. Competitive trampolining also is growing, although there is scant medical literature related to training issues and injuries to assist the physician in guiding and treating these athletes. It is anticipated that with the increased use of trampolines and with the evolution of ever more complex competitive techniques and routines, the potential for catastrophic injuries in competition will increase. This article discusses awareness of the risks and attempts to mitigate injuries. It remains unclear, from an injury risk standpoint, whether trampolines can be used with an acceptable degree of safety.

Diet-induced weight gain increases disease risk via disruption of the innate immune system. Flow cytometry is commonly used to assess the immune system; however, in mice such measurements traditionally require terminal procedures and tissue collection to generate sufficient sample. The present study refined an existing flow cytometry method to reduce the number of mice needed to longitudinally measure monocytes. CD-1 male mice were randomly assigned to one of the three groups: DS (diet-induced weight gain þ sedentary), DE (diet-induced weight gain þ forced treadmill running [total distance 35,755 + 1832 m]) or NS (normal weight gain þ sedentary). DS and DE consumed a 60% fat diet and NS consumed a 10% fat diet ad libitum. Saphenous vein blood samples were collected weekly for a period of six weeks and three-colour flow cytometry was used to measure changes in monocyte (CD11b þ /14 þ ) concentration and cell-surface toll-like receptor 4 (TLR4) expression. DS (18%) and DE (17%) gained more weight than NS (P , 0.001). On a group basis, DS expressed 17% more TLR4 than DE and NS (P ¼ 0.005). The present study demonstrates that a longitudinal survival model can be used to reduce the number of animals needed to complete flow cytometry experiments. Exercise during diet-induced weight prevented some (decreased monocyte TLR4 expression) but not all aspects of innate immune system function.

Web site usability concerns anyone with a web site to maintain. Libraries, however, are often the biggest offenders in terms of usability. In our efforts to provide users with everything they need to do research, we often overwhelm them with sites that are confusing in structure, difficult to navigate, and weighed down with jargon. Dowling College Library recently completed a redesign of its web site based upon the concept of usability. For smaller libraries in particular, this can be a challenge. The web site is often maintained by one or two people and finding the time and resources to conduct a usability study is difficult in that situation. Additional demands of a site redesign, from restructuring page layouts to adding visual appeal, only add to the burden. However, our team of four librarians was able to do it. We focused on vocabulary and organizational structure using a card-sort analysis. This analysis taught us how our users approach the information on our site. Task-based testing confirmed what the card-sort analysis had taught us and smoothed out design problems. Incorporating user feedback at nearly every stage of the process allowed us to create a site that more closely mirrors how our users look for information on our site. This study details how using testing and analyzing results throughout the redesign process created a better, more user-friendly web site.

Severe nonthyroidal illnesses have been associated with increases in nonesterified fatty acids (NEFA) and the dialyzable fraction of thyroxin (T4) in plasma. We have further investigated their possible relationship in severe nonthyroidal illnesses as well as in induced in vivo and in vitro situations involving increased NEFA. We demonstrate that there is no relationship between NEFA and the dialyzable fraction of T4, either in severe nonthyroidal illnesses or in the other situations, unless plasma NEFA concentrations exceed 5 mmol/L in normal persons or 1.7 mmol/L in nonthyroidal illnesses, and that this concentration was not reached in the patients we studied, with one exception. We conclude that NEFA are unlikely to contribute to an inhibition of the binding of T4 to the binding proteins that might be present in plasma of patients with severe nonthyroidal illnesses unless their NEFA concentrations are very high.

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