a b s t r a c tArtisanal raw milk cheeses are highly appreciated dairy products in Brazil and ensuring their microbiological safety has been a great need. This study reports the isolation and characterization of lactic acid bacteria (LAB) strains with anti-listerial activity, and their effects on Listeria monocytogenes during refrigerated shelf-life of soft Minas cheese and ripening of semi-hard Minas cheese. LAB strains (n ¼ 891) isolated from Minas artisanal cheeses (n ¼ 244) were assessed for anti-listerial activity by deferred antagonism assay at 37 C and 7 C. The treatments comprised the production of soft or semi-hard Minas cheeses using raw or pasteurized milk, and including the addition of selected LAB only [Lactobacillus brevis 2-392, Lactobacillus plantarum 1-399 and 4 Enterococcus faecalis (1-37, 2-49, 2-388 and 1-400)], L. monocytogenes only, selected LAB co-inoculated with L. monocytogenes, or without any added cultures. At 37 C, 48.1% of LAB isolates showed anti-listerial capacity and 77.5% maintained activity at 7 C. Selected LAB strains presented a bacteriostatic effect on L. monocytogenes in soft cheese. L. monocytogenes was inactivated during the ripening of semi-hard cheeses by the mix of LAB added. Times to attain a 4 log-reduction of L. monocytogenes were 15 and 21 days for semi-hard cheeses produced with raw and pasteurized milk, respectively. LAB with anti-listerial activity isolated from artisanal Minas cheeses can comprise an additional barrier to L. monocytogenes growth during the refrigerated storage of soft cheese and help shorten the ripening period of semi-hard cheeses aged at ambient temperature.

No abstract

O efeito da fragmentação de habitats tem sido avaliado medindo-se a riqueza e diversidade de determinados grupos funcionais, especialmente insetos visitantes florais. Esses organismos possibilitam conectividade funcional entre plantações e áreas protegidas e a restauração de ambientes alterados. Este estudo objetivou analisar a diversidade de insetos antófilos ao longo de transectos estabelecidos entre mata ciliar e reflorestamento de eucaliptos e exclusivamente entre talhões de eucaliptos, no sul do Brasil. Os insetos coligidos pertencem às ordens Hymenoptera, Lepidoptera, Diptera, Coleoptera e Hemiptera. Comparativamente a outros estudos, a abundância de insetos foi baixa. Vinte e oito espécies foram encontradas em ambos ambientes, enquanto 138 foram exclusivas em um ambiente (61 em mata ciliar/eucaliptos e 77 em eucaliptos/eucaliptos). Os insetos visitaram flores de 31 espécies vegetais, representantes de 20 famílias. Nos dois ambientes foram encontradas poucas espécies dominantes e um grande número representadas por um único indivíduo. A alta diversidade foi similar nos dois ambientes de estudo (H' = 4,084 e H' = 4,018). Entre os insetos, as abelhas mostraram a maior diversidade nos ambientes mata ciliar/eucaliptos (H' = 3,211) e eucaliptos/eucaliptos (H' = 2,8). Considerando-se a similaridade na estrutura da comunidade entre os ambientes, a fauna de insetos antófilos difere quanto à composição de espécies e número de indivíduos das espécies amostradas.

Piglets are highly vulnerable to infections, but colostrum provides them with some protection. The function of colostrum components is unknown, as is if the amount and subsets of leukocytes in colostrum differ between gilts and sows. This study serially characterized leukocyte populations in colostrum for differential leukocyte counts. Differences in humoral and cellular composition of colostrum between 40 gilts and 40 sows (parities orders 3–4) from a commercial herd were examined. Flow cytometry is a useful tool to identify and quantify leukocyte subsets in sow colostrum. Overall, there were no (p ≥ 0.05) parity differences in total macrophages, granulocytes, and T and B cells. However, the sows’ colostrum presented significantly higher (p ≤ 0.05) T lymphocyte subsets than gilts, such as central memory CD4+T cells, effector memory CD4+T cells, and central memory CD8+T cells. Among B-lymphocytes, percentages of SWC7+CD5+ cells were significantly higher in sow colostrum than in that of gilts. As expected, IgG concentrations were significantly higher in sows than in gilts. Colostrum from sows had significantly greater mitogenic activity than colostrum from gilts and this fact can be associated with the potential to accelerate the maturation of a newborn’s gastrointestinal tract. Our findings suggest that parity order may be one among other factors influencing the cell population and, consequently, the immune adaptive response in piglets that induces neutralizing antibodies and cellular immune responses to antigens.

R ESU M ONeste trabalho objetivou-se realizar imagens termográficas da superfície de telhas visando avaliar sua temperatura superficial. Os tratamentos mensurados se constituíram de: T1 -Telha Coppo Venneto Ondulada Cinza; T2 -Telha Coppo Venneto Ondulada Marfim; T3 -Telha Plana Marfim; T4 -Telha Plana Cinza; T5 -Telha de Barro Colonial esmaltada; T6 -Telha Ondulada de Cimento Amianto e T7 -Telha de Barro Francesa. Cada unidade experimental foi composta de três telhas sobrepostas uma sobre a outra, exceto no tratamento de telha ondulada de cimento amianto. O delineamento experimental foi em blocos casualizados com três repetições. Os dados coletados em cada horário foram analisados por meio do modelo de medidas repetidas, utilizando-se o procedimento MIXED do SAS. As temperaturas da superfície inferior das telhas foram muito parecidas ao longo do dia. As telhas com coloração marfim obtiveram melhores valores de temperatura superior e inferior que as telhas com coloração cinza, nas respectivas categorias. A telha de barro colonial obteve maior temperatura da superfície inferior que as demais telhas. Palavras-chave: ambiência animal, conforto térmico, tipos de cobertura, telhadoThermographic analysis of the superficial temperature of roof tiles A B ST R A C T The objective of study was to get thermographic images from the surface of roof tiles to evaluate their tempertures. The evaluated treatments were: T1 -grey Coppo Venneto wave tiles ; T2 -Ivory-colored Coppo Venneto wave tiles; T3 -Ivory-colored, plain tile; T4 -plain, grey roof tile; T5 -glazed clay colonial tile; T6 -Asbestos wave tile T7 -French clay tiles . Each treatment was made up of three tiles which were piled one top of the other, except for the asbestos tiles. The experimental design was in randomized block with three repetitions. The data which were collected in each set time were analyzed through the repetitive measure model, using the MIXED procedure -SAS. The temperatures of the inferior surface of the tiles were very similar throughout the day. The ivory colored tiles had better superior and inferior temperature than the grey-colored ones in their respective category. The glazed, clay colonial tile presented a larger temperature of inferior surface than the other tiles.

Extrato de alecrim e produtos derivados do aipo como agentes naturais potencializadores da qualidade de salames coloniais com diferentes tempos de maturação

Assessments of soil carbon dioxide (CO 2 ), methane (CH 4 ), and nitrous oxide (N 2 O) emissions are critical for determination of the agricultural practices' potential to mitigate global warming. This study evaluated the photoacoustic spectroscopy (PAS) for the assessment of soil greenhouse gases (GHG) RESUMO As avaliações das emissões de dióxido de carbono (CO 2 ), metano (CH 4 ) e óxido nitroso (N 2 O) do solo são fundamentais para a determinação do potencial de práticas agrícolas em mitigar o aquecimento global. Este estudo avaliou a espectroscopia fotoacústica (EFA) para a determinação dos fluxos de gases de efeito estufa (GEE) do solo em comparação com o método padrão de cromatografia gasosa (CG). Dois experimentos de longa duração com diferentes sistemas de

This study was carried out to determine the combining effects of feeding up to 15 mg kg -1 ractopamine (RAC) to physically castrated (PC) pigs or those injected with an anti-GnRF vaccine (IC), on growth performance during suckling, weaning, growing and finishing phases. Out of a total of 1,160 male piglets, 50 % were surgically castrated at age 7, while 50 % underwent immunization against GnRF on the 103 rd and 136 th day of life. A completely randomized design, with two treatments (PC and IC), was used from the 1 st until the 60 th day of life. Afterwards, 792animals, PC and IC, selected according to overall average weight (23.3 ± 0.69 kg) were allotted based on a completely randomized block design with a 2 × 4 factorial arrangement of treatments (castration categories × RAC levels) with 9 replications (11 animals) per main combination effect. IC growing pigs improved feed conversion due to lower average daily feed intake and higher average daily gain (ADG). During the finishing phase, both RAC and immunocastration increased body weight, but the effects were independent. RAC, on its own improved ADG and feed conversion. However, pigs fed 10 mg kg -1 RAC had higher blood lactate compared to those fed lower levels, suggesting that these animals were more susceptible to stress. Muscle depth was influenced only by RAC, whereas fat thickness was lower for IC. Lean meat increased and fat decreased in the shoulder and belly of IC; RAC had a positive effect on belly lean meat only.Either immunocastration or RAC inclusion (at least 5 mg kg -1 ) promoted muscle gain and fat reduction in pig carcass, adding value to pork.