Relatively short-term (2.5 or 5 h) exposure of Wistar rats to oxygen atmosphere at moderate pressure (1.10-1.15 atm) resulted in an increase in LPO level and reduction of antioxidant activity in the blood serum. An increase in malondialdehyde concentration 1 day after termination of the exposure was followed by a decrease in the inhibiting activity of free radical oxidation of liposomal phospholipids induced by Fe(II) ions (100 μm). Malondialdehyde concentration increased by 1.29 times already after 2.5-h exposure and did not changed when the duration of the exposure to oxygen atmosphere was prolonged to 5 h. These data confirm the necessity of using substances potentiating antioxidant defense of the body during exposure to normobaric oxygenation.
We studied the effect of nanostructured clathrate complex 9-phenyl-symm-octahydoselenoxanthene (selenopyran) with β-cyclodextrin on the generation of ОН· radicals in the Fenton system and parameters of oxidative stress in rat liver cells incubated at 37°С for 1 h. The complex inhibits the development of free-radical oxidative processes induced by ROS and the most toxic ОН· radicals, reduces the increased level of ROS induced by prooxidants, and exhibits antioxidant activity.
Exposure of male Wistar rats to oxygen atmosphere at moderate pressure (1.10-1.15 atm) for 4 h resulted in significant, transient, and reversible decrease in hemolytic resistance of peripheral blood erythrocytes. In 2 days after oxygen exposure, the bone marrow released young erythrocytes with enhanced hemolytic resistance as a compensation for oxygen injury. These findings suggest that normobaric oxygen load can be viewed as a stress factor of moderate intensity.
Current trends in the development of new drugs are based on the creation of nanostructured compositions with improved characteristics. One of the approaches to creating a nanoscale form of drugs, in particular 20-hydroxyecdysone, is the synthesis of its clathrate complex with the clathrate-forming arabinogalactan. Thus obtained nanoscale form of 20-hydroxyecdysone provides an improvement in its physico-chemical properties, which leads to increased absorption, increased bioavailability and, ultimately, to reduce therapeutic doses. In the present work, the effect of the created nanoscale form of 20-hydroxyecdysone on the function of the antioxidant defense system, natural resistance, growth and development of Sovetskaya Chinchilla rabbits was studied for the first time. It has been established that the nanoscale form of 20-hydroxyecdysone, when administered to rabbits at doses of 1.0 and 5.0 mg / kg body weight, contributes to a statistically significant increase in serum BASK and LASK levels, increased activity of the enzymatic unit of the antioxidant system, in particular SOD and catalase in erythrocyte lysate. On the contrary, the content of MDA in the blood plasma was statistically significant [P≤0.05 — P≤0.02] was lower in rabbits of the experimental groups compared with the control, which may indicate a decrease in lipid peroxidation processes in animals under the influence of nanoscale form 20-hydroxyecdysone. In the rabbits of the experimental groups, compared with the control, they were statistically significantly higher by 32-38% [P≤0.05–P≤0.01] absolute and average daily weight gain of rabbits. The optimal dose of the nanoscale form of 20-hydroxyecdysone is dose 1, 0 mg / kg rabbit body weight.
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