Although laser desorption mass spectrometry was introduced in the 1960s, the potential of laser mass spectrometry was not realised until the introduction of matrix-assisted laser desorption/ionisation (MALDI) in the 1980s. The technique relies on light-absorbing compounds called matrices that are co-crystallised with the analyte to achieve high ionisation and desorption efficiencies. MALDI offers a lot of advantages and is an indispensable tool in macromolecule analysis. However, the presence of the matrix also produces a high chemical background in the region below m/z 700 in the mass spectrum. Surface-assisted laser desorption/ionisation (SALDI) substitutes the chemical matrix of MALDI for an active surface, which means that matrix interference can be eliminated. SALDI mass spectrometry has evolved in recent years into a technique with great potential to provide insight into many of the challenges faced in modern research, including the growing interest in "omics" and the demands of pharmaceutical science. A great variety of materials have been reported to work in SALDI. Examples include a number of nanomaterials and surfaces. The unique properties of nanomaterials greatly facilitate analyte desorption and ionisation. This article reviews recent advances made in relation to carbon- and semiconductor-based SALDI strategies. Examples of their environmental, chemical and biomedical applications are discussed with the aim of highlighting progression in the field and the robustness of the technique, as well as to evaluate the strengths and weaknesses of individual approaches. In addition, this article describes the physical and chemical processes involved in SALDI and explains how the unique physical and electronic properties of nanostructured surfaces allow them to substitute for the matrix in energy transfer processes.

New mass spectrometry (MS) methods, collectively known as data independent analysis and hyper reaction monitoring, have recently emerged. These methods hold promises to address the shortcomings of data-dependent analysis and selected reaction monitoring (SRM) employed in shotgun and targeted proteomics, respectively. They allow MS analyses of all species in a complex sample indiscriminately, or permit SRM-like experiments conducted with full high-resolution product ion spectra, potentially leading to higher sequence coverage or analytical selectivity. These methods include MS(E), all-ion fragmentation, Fourier transform-all reaction monitoring, SWATH Acquisition, multiplexed MS/MS, pseudo-SRM (pSRM) and parallel reaction monitoring (PRM). In this review, the strengths and pitfalls of these methods are discussed and illustrated with examples. In essence, the suitability of the use of each method is contingent on the biological questions posed. Although these methods do not fundamentally change the shape of proteomics, they are useful additional tools that should expedite biological discoveries.

/npsi/ctrl?lang=en http://nparc.cisti-icist.nrc-cnrc.gc.ca/npsi/ctrl?lang=fr Access and use of this website and the material on it are subject to the Terms and Conditions set forth at http://nparc.cisti-icist.nrc-cnrc.gc.ca/npsi/jsp/nparc_cp.jsp?lang=en NRC Publications Archive Archives des publications du CNRCThis publication could be one of several versions: author's original, accepted manuscript or the publisher's version. / La version de cette publication peut être l'une des suivantes : la version prépublication de l'auteur, la version acceptée du manuscrit ou la version de l'éditeur. Geotechnical Journal, 27, 3, pp. 320-329, 1990-06 An Energy approach for assessing seismic liquefaction potential Law, K. T.; Cao, Y. L.; He, G. N. An energy method for assessing liquefaction potential of granular soils was developed based on laboratory tests and observational data obtained in past major earthquakes. Cyclic triaxial and cyclic simple shear tests were conducted and the results show that a unique relation exists between the dissipated energy during cyclic load and the excess pore pressure that eventually led to liquefaction failure. This unique relation has been combined with an energy attenuation equation to develop a criterion for defining the liquefaction potential of a site. Parameters for the criterion were evaluated from 136 sites involved in 13 major earthquakes over the world. A comparison was made between the energy method and the commonly used stress method. The energy method was found to be simpler to apply and more reliable. Canadian

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A full-scale field experiment has been conducted in an instrumented saprolite slope in Hong Kong. Soil moisture probes, tensiometers, piezometers, inclinometers, earth pressure cells, and a rain gauge were installed in the slope before and during excavation of a cut. This paper presents the results from soil moisture probes, tensiometers, and piezometers to reveal the surface infiltration process. The soil moisture and matric suction results show that the maximum "wetting front" during the wet season was limited to the top 3 m of soil, and a transient perched water table could develop in the soil during a very heavy rainstorm. A new method based on the variation of volumetric water content in the soil is proposed to analyze rainfall infiltration. The results indicate the following phenomena: (i) rainfall infiltration was around 70% of the total rainfall, (ii) the infiltrated water mainly increased the soil moisture content of the soil at shallow depths, and (iii) the surface vertical infiltration had a very limited effect on the permanent groundwater table at depth. The wetting-front analysis shows that the unsaturated wetting band theory can be used to assess the movement of the wetting front in the unsaturated saprolite soil.Key words: rainfall infiltration, moisture content, matric suction, wetting front, field instrumentation.

Objective To determine the effect of labour on free oxygen radical activity in the fetus, as reflected Design Prospective, observational study. SettingMethods by lipid peroxide levels in umbilical cord arterial blood.Department of Obstetrics and Gynaecology, The Chinese University of Hong Kong.Umbilical cord arterial and venous blood samples were collected from singleton term infants delivered by elective caesarean section. Base excess, PO,, pC0, and pH were measured in both samples and compared to identify double venous samples. Cord arterial acid-base balance and concentrations of organic hydroperoxides and malondialdehyde were compared with those obtained from normal vaginal deliveries. ResultsCord arterial blood samples, obtained from cases of uncomplicated labour followed by spontaneous vaginal delivery, had significantly higher lipid peroxide concentrations than those delivered following elective caesarean section. Ths was most marked for malondialdehyde with a median value increased by 105%, whilst organic hydroperoxide was increased by only 27%. Of the acid-base parameters, base excess was increased by 78%, with only minimal changes in pH, $0, and PO,. These differences remained highly significant after including other pregnancy characteristics in multivariate analysis. ConclusionThe findings indicate that high levels of free oxygen radical activity in the fetus are a function of the labour process, as are changes in acid-base balance.

Sodium iodate-induced degenerative changes can be assessed quantitatively and reliably by in vivo retinal imaging using cSLO in adult rats, allowing efficient evaluation of lesions in a large area of retina in longitudinal studies.