Sesbania grandiflora (L.) Pers. is a common traditional medicinal plant used in bronchitis, anaemia, headache, fever, ophthalmia, nasal catarrh, leprosy, inflammation, gout and rheumatism. The present study aimed to assess plant regeneration and plantlets development in vitro using explants of S. grandiflora together with the estimation of total phenolic content and antioxidative activity of various extracts obtained from the plant. Murashige and Skoog (MS) basal medium added with different concentrations of plant growth regulators (PGRs) was used for plant tissue culture, whereas ferric reducing antioxidant power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) assays were used to evaluate the antioxidant potential of different extracts of the plant. In the presence of 6-benzylaminopurine (BAP; 0.1 mg/l), the highest level (85.41%) of seed germination was achieved while the highest callus formation (96.6%) was recorded with 2,4-dichlorophenoxyacetic acid (2,4-D; 0.5 mg/l). In addition, the highest shoot induction, shoot formation and shoot elongation were observed with BAP (0.1 mg/l), indole-3-butyric acid (3 mg/l) and naphthaleacetic acid + BAP (0.4 + 0.2 mg/l) respectively. The extract of dried calluses showed highest contents of proline (110.94 mg/g), phenol (16.42 mg/g) and flavonoid (22.22 mg/g), and also highest antioxidant potential with FRAP and DPPH assays. From the present study, we may conclude that the MS basal medium supplemented with PGRs is effective for the commercial production of S. grandiflora.Keywords: Antioxidant activity, in vitro micropropagation, phenolic content, plant growth regulators, Sesbania grandiflora.SESBANIA GRANDIFLORA (L.) Pers., commonly known as hummingbird tree of the family Leguminosae, is a popular traditional medicinal plant used in bronchitis, anaemia, headache, fever, ophthalmia, nasal catarrh, leprosy, inflammation, gout and rheumatism 1 . The dried leaves are used as tea and also as an antibiotic, antitumour and contraceptive. The juice obtained from fresh leaves was found effective in bruises 2 . The seeds of the plant are rich in protein while seed oil is a potent antioxidant agent. Various studies confirmed the presence of phytosterols in the seed oil which shows anti-inflammatory, analgesic and antipyretic activities. The presence of high phytosterol content makes it an important plant for the commercial production of steroidal hormones and cosmetics 3 . The flowers of S. grandiflora contain tannin and other nutritious contents. Moreover, its astringent nature also contributes in antimicrobial activity 4 . The process of plant tissue culture is considered to be highly valuable in terms of commercial production of a medicinal plant, in which the aseptic culture of cells and organs can be achieved under in vitro conditions 5 . Several compounds with a varied array of structures influence the tissue growth and morphogenesis under exposed atmosphere. Besides, this technique is useful for conserving medicinal plants and enhancing their secondary metabolites of industr...

No abstract

Aims: The increase of potential side effects and safety concerns of conventional medicaments and irrigants have led to the recent popularity of herbal alternatives. The present in vitro study was undertaken to evaluate the antimicrobial efficacy of 3% NaOCl, Metronidazole, Curcuma longa and Mimusops elengi against Enterococcus faecalis. Settings and Design: The experimental materials were prepared and allotted into four groups (Group 1-NaOCl, Group 2-Metronidazole, Group 3-Curcuma longa, Group 4-Mimusops elengi). Methods and Materials: Agar well diffusion test was done to evaluate the antimicrobial efficacy of various intracanal irrigants. Enterococcus faecalis was used as a test organism. The inoculums of Enterococcus faecalis was streaked on the agar plate. Using punch, wells each measuring 6mm in diameter and 4mm in depth were made and 50 micro liter of the experimental irrigant was pippeted into each well. The plates were placed in Carbon di oxide incubator at 37 degree Celsius for 48 hours and the zones of inhibition were measured. Statistical analysis used:The results were statistically analysed using one way ANOVA and Tukey's HSD Results: Highest inhibitory zone against E. faecalis was shown by 0.5% of metronidazole followed by 25% Mimusops elengi, 3% Sodium hypochlorite and the least is shown by 12.5 % Curcuma longa Conclusions: Considering the undesirable side effects of sodium hypochlorite,metronidazole and herbal irrigants; mimusops elengi and Curcuma longa could be considered as an alternative

No abstract

No abstract