Sesbania grandiflora (L.) Pers. is a common traditional medicinal plant used in bronchitis, anaemia, headache, fever, ophthalmia, nasal catarrh, leprosy, inflammation, gout and rheumatism. The present study aimed to assess plant regeneration and plantlets development in vitro using explants of S. grandiflora together with the estimation of total phenolic content and antioxidative activity of various extracts obtained from the plant. Murashige and Skoog (MS) basal medium added with different concentrations of plant growth regulators (PGRs) was used for plant tissue culture, whereas ferric reducing antioxidant power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) assays were used to evaluate the antioxidant potential of different extracts of the plant. In the presence of 6-benzylaminopurine (BAP; 0.1 mg/l), the highest level (85.41%) of seed germination was achieved while the highest callus formation (96.6%) was recorded with 2,4-dichlorophenoxyacetic acid (2,4-D; 0.5 mg/l). In addition, the highest shoot induction, shoot formation and shoot elongation were observed with BAP (0.1 mg/l), indole-3-butyric acid (3 mg/l) and naphthaleacetic acid + BAP (0.4 + 0.2 mg/l) respectively. The extract of dried calluses showed highest contents of proline (110.94 mg/g), phenol (16.42 mg/g) and flavonoid (22.22 mg/g), and also highest antioxidant potential with FRAP and DPPH assays. From the present study, we may conclude that the MS basal medium supplemented with PGRs is effective for the commercial production of S. grandiflora.Keywords: Antioxidant activity, in vitro micropropagation, phenolic content, plant growth regulators, Sesbania grandiflora.SESBANIA GRANDIFLORA (L.) Pers., commonly known as hummingbird tree of the family Leguminosae, is a popular traditional medicinal plant used in bronchitis, anaemia, headache, fever, ophthalmia, nasal catarrh, leprosy, inflammation, gout and rheumatism 1 . The dried leaves are used as tea and also as an antibiotic, antitumour and contraceptive. The juice obtained from fresh leaves was found effective in bruises 2 . The seeds of the plant are rich in protein while seed oil is a potent antioxidant agent. Various studies confirmed the presence of phytosterols in the seed oil which shows anti-inflammatory, analgesic and antipyretic activities. The presence of high phytosterol content makes it an important plant for the commercial production of steroidal hormones and cosmetics 3 . The flowers of S. grandiflora contain tannin and other nutritious contents. Moreover, its astringent nature also contributes in antimicrobial activity 4 . The process of plant tissue culture is considered to be highly valuable in terms of commercial production of a medicinal plant, in which the aseptic culture of cells and organs can be achieved under in vitro conditions 5 . Several compounds with a varied array of structures influence the tissue growth and morphogenesis under exposed atmosphere. Besides, this technique is useful for conserving medicinal plants and enhancing their secondary metabolites of industr...
Objective: In this present study attempt was made to formulate transdermal patches contains phytoconstituents. The naturopathy does not involve any adverse effects. Methods: Hibiscus rosasinensis aquous extracts was prepared. Transdermalpatches were prepared using drug with two different polymers. The prepared transdermal films were evaluated for their physiochemical characteristics such as physical appearance, weight uniformity, thickness, folding endurance; moisture content, surface pH, Tensile strength. The in-vitro diffusion study was carried out using rat membrane. These parameters indicates the successful release of drug from the fabricated patch. Results: With the overall observation it was concluded that the. Conclusion: Fabrication of transdermal patch is successfully worked and subjected to diffusion study. Diffusion studies are carried out by using a fresh rat membrane. Phosphate buffer (6.6) is used as a solvent. Samples are collected for 24 h and absorbance is measured by using UV spectrophotometer at 226 nm. It showed the successful release of drug from the fabricated patch.
Objective: The present study was aimed to evaluate the antidiabetic activity of ethanolic extract of the whole aerial plant of Portulaca grandiflora Hook on streptozotocin (STZ)-induced diabetic rats. Methods: Experimental diabetes was induced by a single dose of intraperitoneal injection of STZ (150 mg/kg). Adult male Wister albino rats were divided into five groups; normal control, diabetic control, diabetic glibenclamide (5 mg/kg), diabetic P. grandiflora H. extract (200 mg/kg), and diabetic P. grandiflora H. extract (400 mg/kg) for 21 days and analyzed for body weight (BW) and blood glucose. Results: The STZ-treated diabetic control rats showed a significant increase in blood glucose with a concomitant decrease in BW. Oral administration of P. grandiflora H. extract (200 and 400 mg/kg) and glibenclamide (5 mg/kg) for 21 days showed a significant reduction in blood glucose levels and elevation in the bodyweight studies as compared to control and glibenclamide-treated rats. Conclusion: The results of the present study showed that a potent antidiabetic activity was present in the aerial part of plant P. grandiflora H. extract.
The polycystic ovarian syndrome affects women of all age groupsis mainly due to hyperinsulinemia and hyperandrogenism.The insulin action in the ovaries is mediated by binding to its receptor namely IRS (Insulin receptor substrate).The present investigation is undertaken to select a suitable antagonistic ligand from the bioactive phyto components of Tinosporacordifolialeaves to inhibit IRS1 and IRS 2 receptors to prevent the binding of insulin and subsequent hyperandrogenism.The phyto ligands used for the study were obtained from the previous literature and the IRS1 and IRS 2 receptor protein structure were retrieved from PDB (protein Data bank). Using the Corina 3D converter the ligand 3D structures were obtained. Molecular docking analysis was performed through Patch dock server to select a suitable ligand based on docking score for the IRS1 and IRS 2 receptor protein.Three ligands namely Berberine, Rumphioside I and Syringin showed better docking score among the ligand selectedand their inhibitory activity were analysed by intermolecular interactions using “LIGPLOT” analysis. It can be concluded that these three ligands can be used for the successful treatment of PCOS after proper preclinical and clinical studies.
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