Photocatalysis with anatase Titanium dioxide (TiO 2 ) under ultraviolet A (UVA) has a well recognized bactericidal effect. There have been a few reports, however, on the effects of photocatalysis on bio-implant-related infections. The purpose of present study was to evaluate the photocatalytic bactericidal effects of anatase TiO 2 on Staphylococcus aureus (S. aureus) associated with surgical site infections.TiO 2 films were synthesized on commercially pure titanium substrates and SUS316 stainless steel using a plasma source ion implantation method followed by annealing. The chemical composition of the surface layers was determined using GXRD and XPS. The disks were seeded with cultured S. aureus and exposed to UVA illumination from black light. The bactericidal effect of the TiO 2 films was evaluated by counting the survived colonies statistically.A structural gradient anatase type TiO 2 layer formed on all substrates. The viability of the bacteria on the photocatalytic TiO 2 film coated on titanium was suppressed to 7.0% at 30 minutes and 5.5% at 45 minutes, whereas that on a similarly coated stainless steel was suppressed to 45.8% at 30 minute and 28.6% at 45 minutes (ANOVA: p < 0.05). Complete bacterial inactivation was achieved after 90 minutes on titanium and after 60 minutes on stainless steel. The photocatalytic bactericidal effect of TiO 2 is useful for sterilizing the contaminated surfaces of bioimplants.
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