Effect of adrenomedullin on aldosterone secretion by dispersed rat adrenal zona glomerulosa cells

Yamaguchi, T; Baba, Koumei; Doi, Yutaka; Yano, Katsusuke

doi:10.1016/0024-3205(94)00903-1

Cited by 161 publications

(83 citation statements)

References 8 publications

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“…Immunocytochemistry studies have shown that adrenomedullin is widely distributed in the endocrine and neuroendocrine system, where it may play a role in the control of systemic and local circulation (4), and of humoral secretion. It has been demonstrated that adrenomedullin inhibits the secretion of adrenocorticotrophin (ACTH) (5), aldosterone (6), and insulin (7) and its secretion is stimulated by the thyroid hormones, progesterone and dexamethasone (8).…”

Section: Introductionmentioning

confidence: 99%

Adrenomedullin production is increased in normal human pregnancy

Iorio¹,

Marinoni²,

Letizia

et al. 1999

European Journal of Endocrinology

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Objective: Adrenomedullin, a recently discovered vasoactive peptide originally identified in pheochromocytoma, has been found to be increased in the plasma of pregnant women at term. This study was designed to elucidate whether adrenomedullin secretion is dependent on gestational age and the possible source and function of this peptide in human pregnancy. Study design: Adrenomedullin concentrations were determined by RIA in amniotic fluid and maternal plasma obtained from 110 pregnant women between 8 and 40 weeks of gestation. Subjects were stratified into five groups according to gestational age. In term patients (n¼15), adrenomedullin was also measured in the umbilical artery and vein separately. Results: High concentrations of adrenomedullin were present in plasma and amniotic fluid samples from patients in the first, second and third trimester. There was no significant difference in mean maternal plasma concentration of adrenomedullin between the five patient groupings. Amniotic fluid adrenomedullin concentrations decreased from 81.2Ϯ11.7 pg/ml at 8-12 weeks of gestation to 63.7Ϯ6.0 pg/ml at 13-20 weeks of gestation and then increased at 21-28 weeks of gestation to 99.1Ϯ10.4 pg/ml. A further increase was found in samples collected after 37 weeks of gestation (132.6Ϯ10.1 pg/ml). In the umbilical vein, adrenomedullin concentration was higher (P<0.05) than in the artery (65.7Ϯ6.1 pg/ml and 48.5Ϯ5.2 pg/ml respectively), suggesting that adrenomedullin in the fetal circulation derives from the placenta.Conclusions: Our results demonstrate the presence of adrenomedullin in maternal plasma and amniotic fluid throughout gestation, and show that its production starts very early in gestation, suggesting that this hormone may have an important role in human reproduction, from implantation to delivery.

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Section: Introductionmentioning

confidence: 99%

Adrenomedullin production is increased in normal human pregnancy

Iorio¹,

Marinoni²,

Letizia

et al. 1999

European Journal of Endocrinology

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“…Recent studies have investigated the actions of adrenomedullin on the rat adrenal zona glomerulosa, but have suggested that the actions of adrenomedullin may be dependent on the tissue preparation used, as there have been reports of inhibition of aldosterone secretion in dispersed cells (Mazzocchi et al 1996b, Yamaguchi et al 1995, but stimulation of aldosterone secretion both in intact capsules and in the intact perfused rat adrenal preparation (Mazzocchi et al 1996a, Kapas & Hinson 1996. Adrenomedullin was originally identified by its ability to increase the concentrations of cAMP in platelets.…”

Section: Introductionmentioning

confidence: 99%

“…Adrenomedullin was originally identified by its ability to increase the concentrations of cAMP in platelets. To date, studies on its intracellular actions in the adrenal cortex have implicated both cAMP and the calcium messenger system (Yamaguchi et al 1995, Kapas & Hinson 1996. It has not been determined whether adrenomedullin may be produced in the adrenal cortex, although previous immunocytochemical studies located it only to the medulla (Washimine et al 1995) and the zonal distribution of adrenomedullin receptors has not been investigated.…”

Section: Introductionmentioning

confidence: 99%

Local production and action of adrenomedullin in the rat adrenal zona glomerulosa

Kapas¹,

Martı́nez²,

Cuttitta³

et al. 1998

Journal of Endocrinology

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This study was designed to investigate the synthesis and action of adrenomedullin in the rat adrenal gland. The results obtained from in situ hybridization and immunocytochemical studies suggest that adrenomedullin is synthesized not only in the medulla, but also within the zona glomerulosa of the rat adrenal cortex. Findings from in situ hybridization and binding studies also suggested that specific adrenomedullin receptors are expressed in the zona glomerulosa, and that low levels are present in the inner zones of the cortex. The K d of the zona glomerulosa adrenomedullin receptor (5·5 nmol/l) suggests that it may respond to locally produced adrenomedullin rather than circulating concentrations of the peptide, which are in a lower range. It was found that adrenomedullin acted on zona glomerulosa cells in vitro to stimulate aldosterone release and cAMP formation, but in this tissue did not stimulate inositol phosphate turnover. The effect of adrenomedullin on aldosterone secretion was significantly attenuated by a protein kinase A inhibitor, suggesting that cAMP mediates the effects of adrenomedullin on aldosterone secretion. Adrenomedullin did not significantly affect the response of zona glomerulosa cells to stimulation by either ACTH or angiotensin II. Adrenomedullin did not affect the release of catecholamines, either adrenaline or noradrenaline, by intact adrenal capsular tissue. These data suggest that both adrenomedullin and its specific receptor are expressed in the rat adrenal zona glomerulosa, leading to the hypothesis that adrenomedullin may have an autocrine/paracrine role in the regulation of the rat adrenal zona glomerulosa.

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“…With few exceptions (15,23), all previous investigations agree that AM selectively inhibits Ang-II-and K -stimulated aldosterone secretion from dispersed rat (24)(25)(26) and human ZG cells (27)(28)(29)(30), without affecting either basal or ACTH-stimulated secretion or the secretory activity of ZF/R cells. Accordingly, in vivo studies have shown that AM administration does not affect basal aldosterone plasma concentration in control rats, but significantly decreases it in animals given a sodium-deficient diet (31,32), and in two-kidney, one clip hypertensive rats (33).…”

Section: Discussionmentioning

confidence: 71%