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Abstract:The present work deals with preliminary in-vitro studies to validate the traditional claims of medicinal properties in consuming Curcuma flowers to make the person less susceptible to diseases and the estimation of bioactive compounds responsible for antioxidant activity present. The anti-proliferative activity of aqueous extracts of budding flowers, leaves, full bloomed flowers and mixture of all parts of Curcuma angustifolia was studied using MDCK, Vero and HeLa cell lines. The inhibitory activity in all the three cell lines was found to be dose dependent. The extract of leaves and budding flowers exhibited higher inhibitions in MDCK and Vero cells while the full bloomed flowers extract showed higher inhibitory activities in HeLa cells. The bioactive compounds-total phenolics content ranged from 56.96 to 86.77 mg GEA/100 gm which was highest in full bloomed flowers, total flavonoids and the free radical scavenging activity content ranged from 84.74 to 166.11 mg CE/100 g and 43.91 to 64.02 mg AEAC/100 gm respectively, with maximum content in budding flowers. The result showed potential anti-proliferative and antioxidant properties that substantiate the belief of the indigenous people to include the plant in their dietary habits which could be exploited for use in preparation of herbal drugs and supplements.

MicroRNAs (miRNAs) are small non-coding RNA, whose differential expression has been involved in the development and progression of cancers, including prostate cancer (PCa). In recent studies circulating miRNAs have been demonstrated to have the potential as non-invasive biomarkers in various types of cancer. The aim of this study was to investigate the serum circulating miRNA-103 could be used in diagnosis of prostate cancer in North-Eastern India patient population. To evaluate this study blood samples were collected from prostate cancer patients and healthy controls following ethical approval and informed consent. The samples were reverse transcribed using stem loop primers and expression levels of miRNA-103 were determined using real time PCR. Differential expressions of miRNA-103 for each case were used to compare PCa serum with healthy serum samples. Serum circulating miR-103 levels was significantly down-regulated in the PCa patients comparison with healthy control. The serum circulating miRNA-103 could be potential for the diagnosis of PCa in North-Eastern India Population. Citation Format: Khanmi Kasomva, Ignacimuthu Savarimuthu, Gabriel Michael Paulraj, Arnab Sen, Stephen Sailo, Ngachan SV. Serum circulating microRNAs as potential biomarkers for prostate cancer [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2017; 2017 Apr 1-5; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2017;77(13 Suppl):Abstract nr 5454. doi:10.1158/1538-7445.AM2017-5454