Feline parvovirus infection (FPV) is the most prevalent cause of fatal gastroenteritis in cats. Only a few reports of FPV infection in cats have been found in Egypt. Therefore, the current study was designed to detect FPV diseased cats in 3 Egyptian provinces. FPV diseased cats was detected based on clinical examination, viral antigen detection using a commercially available screening Ag rapid test kit, and molecular detection of VP-2 gene using specific primers. A total of 100 diseased cats were studied, with a variety of breeds, ages, and sexes. The cats suffered from some clinical anorexia, lethargy, fever, abdominal pain, vomiting, and/or mucoid to bloody diarrhea. These clinical signs were suggestive of FPV infection. The rapid screening test detected the infection in 35 out of 100 diseased cats, while PCR revealed the presence of the FPV infection in 43 diseased cats. Prevalence of FPV infection in Suhag, Assuit, and Cairo was 56.25%, 24.2%, and 25.7%, using rapid test and 62.5%, 27%, and 40% using PCR, respectively. Cats under the age of one year, mainly between the ages of one to six months, and unvaccinated cats had higher infection rates, particularly in the spring and winter. In addition, the relationship between clinical signs of the disease and molecular detection of FPV was extensively investigated. It could be concluded that FPV circulated among Egyptian cat populations, and the knowledge obtained in this study would help initiate preventive measures in the study areas.