The objective of this study was to evaluate if the plant extract could provide antifungal potential against some pathogenic fungi. Extract of Dichloromethane solvents was used for disc diffusion assay. The inhibitory concentration of the extract was performed by broth dilution method and zone of inhibition was studied by disc diffusion method at the concentration of 25, 50, 100, 250, 500 and 1000ppm in DMSO. Fluconazole was used as the reference control for antifungal study. The extract showed maximum inhibition potential of zone of inhibition against most of the pathogen (Aspergillus niger, Aspergillus flavus, Candida tropicalis and Fusarium oxysporium) used at concentration 25ppm to 250ppm with zone of inhibition (3.45±0, 3.33±0.12, 3.07±0.05 and 2.97±0.10mm respectively). The extract showed minimum inhibition potential against Fusarium oxysporium in all the concentration when compared with the control as well as to the other pathogens. Aspergillus niger and Aspergillus flavus was found to be more sensitive to Dichloromethane leaf extract followed by Candida tropical and lastly fusarium oxyspurium. The Present study indicates the potential usefulness of Dichloromethane leaf extract of Leptadenia hastata as antifungal agent.
Introduction: Medicinal plants represent a rich source of antimicrobial agents. Plants are used medicinally in different countries and they are source of many potent and powerful drugs. A wide range of medicinal plant parts is used for extract as raw drugs. Objective: The antifungal activity of different medicinal plant Methanol extracts for three different plant was selected for this studies. These plants were Leptadenia hastata, Barringtonia racemosa and Barringtonia asiatica. The aim of this test is to find out the efficacy of the plants against some selected pathogen. this may help in the discovery of new chemical classes of antifungal and also serve as selective probiotic agents, when administered in adequate amounts, confer a health benefit on the host. Material and methods: Leptadenia hastata, Barringtonia asiatica and Barringtonia racemosa extracts were tested for potential antifungal properties. The roots were extracted with methanol solvent and then vaporized to give respective extracts. The antimicrobial activity against Aspergillus niger, Aspergillus flavin, Candida tropicalis and Fusarium oxysporium (fungi) was determined by agar desk method. The optical densities of the broth using UV mini spectrophotometer and zone of inhibition by the crude extracts were determined. Results: the results showed the efficacy of the plants extracts at different concentration with varying data, higher rate of inhibition was observed in all the extract against all the fungi species. However, extract from Leptadenia hastata showed more significant inhibition with diameter rate with 3.38±0.08 to 5.00±0.09 days five for 25ppm to 250ppm, while Barringtonia racemosa and Barringtonia asiatica lesser compared to Leptadenia hastata as against the pathogen at the same concentration and same time frame. Conclusion: It is showed that all plants extract was found maximally affected against the activity of Aspergillus niger, Aspergillus Flavin, Candida tropicalis and Fusarium oxysporium and aggressively inhibits the growth of species in various plant extracts. This may help in the discovery of new chemical classes of antifungal as well as antibiotic that could serve as selective agents for the maintenance of human health and may provide biochemical tools for the study of fungal infectious diseases.
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