Halioglobus japonicus gen. nov., sp. nov. and Halioglobus pacificus sp. nov., members of the class Gammaproteobacteria isolated from seawater Two coccoid, non-motile bacteria were isolated from seawater in the north-western Pacific Ocean near Japan. The two strains, designated S1-36 T and S1-72 T , were Gram-negative, obligately aerobic, heterotrophic and catalase-negative. They were able to reduce nitrate to nitrogen. Both strains required NaCl for growth, with optimum growth in 2 % NaCl, and grew at 15-30 6C, with optimum growth at 20-25 6C. Genomic DNA G+C contents of strains S1-36 T and S1-72 T were 59.6 and 59.4 mol%, respectively. The predominant isoprenoid quinone was Q-8 and major cellular fatty acids were C 16 : 1 v7c, C 18 : 1 v7c and C 17 : 1 v8c. Analyses of 16S rRNA gene sequences revealed that strains S1-36 T and S1-72 T were related to each other (96.1 % sequence similarity) and both strains showed 92.3-94.7 % sequence similarity with members of the genus Haliea. On the basis of phenotypic and phylogenetic features, strains S1-36 T and S1-72 T should be classified as representatives of two novel species in a new genus, Halioglobus gen. nov., within the class Gammaproteobacteria. The names proposed are Halioglobus japonicus sp. nov., the type species of the genus, with S1-36 T (5NBRC 107739 T 5KCTC 23429 T ) as type strain, and Halioglobus pacificus sp. nov., with S1-72 T (5NBRC 107742 T 5KCTC 23430 T ) as type strain.

Aureitalea marina gen. nov., sp. nov., a member of the family Flavobacteriaceae, isolated from seawater An irregular rod-shaped, non-gliding, yellow-pigmented bacterium was isolated from seawater from the western North Pacific Ocean near Japan. The strain, designated S1-66 T , was Gram-negative, obligately aerobic, heterotrophic and oxidase-positive. Growth occurred in the presence of 1-4 % NaCl, with optimum growth at 2 % NaCl. The strain grew at 15-30 6C, with optimum growth at 20-25 6C. The G+C content of genomic DNA was 48.1 mol% (HPLC). The predominant isoprenoid quinone was MK-6 and the major cellular fatty acids were iso-C 15 : 0 (26.4 %), iso-C 15 : 1 (20.3 %) and iso-C 17 : 0 3-OH (14.2 %). Phylogenetic trees generated by using 16S rRNA gene sequences revealed that strain S1-66 T belongs to the family Flavobacteriaceae and showed 94.2 % sequence similarity to the most closely related type strain, Ulvibacter antarcticus IMCC3101 T . On the basis of phenotypic and phylogenetic features, S1-66 T is classified in a novel genus and species within the family Flavobacteriaceae, for which the name Aureitalea marina gen. nov., sp. nov. is proposed. The type strain of Aureitalea marina is