Halioglobus japonicus gen. nov., sp. nov. and Halioglobus pacificus sp. nov., members of the class Gammaproteobacteria isolated from seawater

Abstract

Halioglobus japonicus gen. nov., sp. nov. and Halioglobus pacificus sp. nov., members of the class Gammaproteobacteria isolated from seawater Two coccoid, non-motile bacteria were isolated from seawater in the north-western Pacific Ocean near Japan. The two strains, designated S1-36 T and S1-72 T , were Gram-negative, obligately aerobic, heterotrophic and catalase-negative. They were able to reduce nitrate to nitrogen. Both strains required NaCl for growth, with optimum growth in 2 % NaCl, and grew at 15-30 6C, with optimum growth at 20-25 6C. Genomic DNA G+C contents of strains S1-36 T and S1-72 T were 59.6 and 59.4 mol%, respectively. The predominant isoprenoid quinone was Q-8 and major cellular fatty acids were C 16 : 1 v7c, C 18 : 1 v7c and C 17 : 1 v8c. Analyses of 16S rRNA gene sequences revealed that strains S1-36 T and S1-72 T were related to each other (96.1 % sequence similarity) and both strains showed 92.3-94.7 % sequence similarity with members of the genus Haliea. On the basis of phenotypic and phylogenetic features, strains S1-36 T and S1-72 T should be classified as representatives of two novel species in a new genus, Halioglobus gen. nov., within the class Gammaproteobacteria. The names proposed are Halioglobus japonicus sp. nov., the type species of the genus, with S1-36 T (5NBRC 107739 T 5KCTC 23429 T ) as type strain, and Halioglobus pacificus sp. nov., with S1-72 T (5NBRC 107742 T 5KCTC 23430 T ) as type strain.

A taxonomic framework for emerging groups of ecologically important marine gammaproteobacteria based on the reconstruction of evolutionary relationships using genome-scale data

“… b The DNA G + C content value of Dasania marina was deduced from the genome sequence, the value give in the literature is 37 mol%. The superscript numbers indicate the following used references: 1, Lee et al ( 2007 ); 2, Suarez et al ( 2014 ); 3, Urios et al ( 2011 ); 4, Cheng et al ( 2015 ); 5, Iwaki et al ( 2012 ); 6, Yoon et al ( 2009 ); 7, Chen et al ( 2011 ); 8, Park et al ( 2014 ); 9, Romanenko et al ( 2010 ); 10, Spring et al ( 2013 ); 11, Park et al ( 2012 ); 12, Baba et al ( 2011 ); 13, Zhang et al ( 2012 ); 14, Kämpfer et al ( 2012 ); 15, Jeong et al ( 2013 ); 16, Vashist et al ( 2013 ); 17, Oh et al ( 2010b ); 18, Jang et al ( 2011a ); 19, Su et al ( 2013 ); 20, Lo et al ( 2014 ) . …”

Taxonomy and evolution of bacteriochlorophyll a-containing members of the OM60/NOR5 clade of marine gammaproteobacteria: description of Luminiphilus syltensis gen. nov., sp. nov., reclassification of Haliea rubra as Pseudohaliea rubra gen. nov., comb. nov., and emendation of Chromatocurvus halotolerans

“…Some of these strains were described as mixotrophs containing photosynthetic pigments [6,15] or proteorhodopsin (PR) [16]. In contrast, no photosynthetic pigments were reported in members of the genus Haliea [17-19] or Halioglobus [20]. The recently described non-pigmented species Dasania marina [21] and “Oceanicoccus sagamiensis” [22] are most likely affiliated to the BD1-7 lineage, whereas representatives of the more distantly related genera Spongiibacter [23] and Zhongshania [24] form a third phylogenetic branch.…”

The Microbial Community of a Terrestrial Anoxic Inter-Tidal Zone: A Model for Laboratory-Based Studies of Potentially Habitable Ancient Lacustrine Systems on Mars

“…Molecular analysis of the analogue site demonstrated that the bacterial community was dominated by the three taxa: Halioglobus , Caldilineaceae and Alteromonadaceae , which are commonly found in marine environments [ 92 , 93 , 94 , 95 , 96 , 97 ]. The bacterial taxa identified were diverse and consisted of both obligate and facultative anaerobes, with varying metabolism, for example chemolithoautotrophic and chemoorganoheterotrophic.…”