Steady-state and transient intracapillary temperature gradients are measured by Raman microthermometry during capillary zone electrophoresis. The axial temperature gradient away from a heat sink extends for several millimeters, depending upon the contact of the capillary and heat sink. In free-air convection, small radial gradients, 2-4 degrees C from the center of the lumen to the wall, are observed at 0.85 kW/cm3. The temperature profile is adequately described by a parabola. With forced cooling, the center-wall temperature difference is less than 1 degrees C. The time to steady-state temperature after power-on is found to be 20 s. The measurements are compared to the results of heat transport calculations.
The Hadamard transform Raman microscope equipped with a two-dimensional video detector is inherently capable of obtaining images at several different Raman frequencies simultaneously. In this communication, we describe the necessary software to exploit this multispectral capability. We apply multispectral imaging to polymer laminates and to removal of artifacts from Raman images.
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